IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhao-2021-10 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zhao-2021-13 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zhao-2021-16 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa tag visualisation tag visualisation Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zhao-2021-12 in vitro In vitro frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zhao-2021-14 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zhao-2021-15 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. light microscopy light microscopy predetermined predetermined participant predetermined Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zhao-2021-17 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus zhao-2021-18 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhao M., Yu Y., Sun LM., Xing JQ., Li T., Zhu Y., Wang M., Yu Y., Xue W., Xia T., Cai H., Han QY., Yin X., Li WH., Li AL., Cui J., Yuan Z., Zhang R., Zhou T., Zhang XM., Li T. Virus - Publications including interactions involving viral proteins It is not known if the Viral RNA sequences obtained from TSINGKE Biotech were In Vitro or chemically synthesised as the TSINGKE Biotechnology web site is in Chinese only. Nature communications 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus 60-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA vrna_3'utr rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 55-nt_vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 5′ppp-dsrna ds rna ds rna double stranded ribonucleic acid synthetic construct synthetic construct GCAUGCGACCUCUGUUUGA dsrna-3'utr ds rna ds rna double stranded ribonucleic acid SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV poly(i:c) poly(I:C) 5'-Inosinic acid, homopolymer, complex with 5'-cytidylic acid homopolymer (1:1) PI:C Poly C poly I Poly I-C poly I:C Poly I:poly C Poly IC Poly rI:poly rC Poly(cytidylic acid)-poly(inosinic acid) Poly(I).poly(C) Poly(I).poly(C) complex poly(I).poly(C) Poly(I+C) Poly(rI).(rC) Poly(rI).poly(rC) Polycytidylic-polyinosinic acid Polyinosinic acid:polycytidylic acid Polyinosinic-polycytidylic acid polyinosinic:polycytidylic acid Polyriboinosinic-polyribocytidylic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 20-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 30-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 50-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV 40-nt vrna rna ribonucleic acid RNA rna SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV gallocatechin gallate Gallocatechin gallate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 60_nt vrna-n-1 4053931 N/A 4053939 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0DTC9:p.Arg203_Gly204delinsLysArg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1 C/D,3D, 4C, Supp. figure 4J, 5E None of the truncated N variants can undergo LLPS (Fig. 3d–h) 60_nt vrna-n-3 4053931 N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0DTC9:p.Arg203_Gly204delinsLysArg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4053939 unspecified role unspecified role ancillary ancillary nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P0DTC9:p.Arg203_Gly204delinsLysArg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1 E,3G, 4F, Supp. fig 3C, 4 A/D/G None of the truncated N variants can undergo LLPS (Fig. 3d–h) pH > ~7.0 [NaCl] ~>physiological (150mM/L) n-vrna_3'utr-2 4053931 N/A 4053941 unspecified role unspecified role ancillary ancillary purified purified vRNA_3'UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. figure 3C n-vrna_3'utr-1 4053931 N/A 4053942 unspecified role unspecified role ancillary ancillary purified purified 55nt-vrna (5' region) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. figure 3C 5ppp_dsrna-n-1 4053931 N/A 4053943 unspecified role unspecified role ancillary ancillary purified purified 5’ guanosine-triphosphate rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false synthetic construct synthetic construct N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. figure 3C n-vrna_3'utr-3 4053931 N/A 4053944 unspecified role unspecified role ancillary ancillary purified purified vRNA_3'UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. figure 3C n-poly(i:c)-1 4053931 N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053945 unspecified role unspecified role ancillary ancillary purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. figure 3C 20_nt vrna-n-1 4053931 N/A 4053946 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. figure 4J 30nt vrna-n-1 4053931 N/A 4053947 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supp. figure 4J 50_nt vrna-n-1 4053931 N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053948 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Supp. figure 4J 40_nt vrna-n-2 4053931 N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053949 unspecified role unspecified role neutral component neutral component nsp1_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Supp. figure 4J n-n-63 4053932 N/A 4053940 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4053940 unspecified role unspecified role neutral component neutral component C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 2 B/D, 6A/D, Supp. figure 7 D/F poly(I:C) -CHEBI:84491 vRNA 3′ UTR (NC_045512.2 residues 29675-29903). Transfection of N-expressing cells with poly(I:C) or the vRNA (3′ UTR), resulted in the formation of N protein condensates. GCG (-)-gallocatechin gallate, a polyphenol from green tea, CHEBI:156271. 60-nt vrna (NC_045512.2, nsp1_region residues 335-394) . 5’ triphosphate_5’ppp-dsRNA (5'- pppGCAUGCGACCUCUGUUUGA-3') 55nt-vRNA (5' region)[residues 1-55, NC_045512.2] dsRNA -3'prime (NC_045512.2 residues 29675-29903) EGCG - Epigallocatechin gallate -the most abundant catechin in (green) tea CHEBI:4806. EGCG had much weaker effect on the inhibition of N-RNA condensation than GCG (-)-gallocatechin gallate. n-n-64 4053932 N/A 4053941 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region cy5 label cy5 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false vRNA_3'UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4053940 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false 2C, 6 C Transfection of N-expressing cells with poly(I:C) or the vRNA (3′ UTR), resulted in the formation of N protein condensates. GCG (-)-gallocatechin gallate (GCG), a polyphenol from green tea, CHEBI:156271. 55_nt_vrna-n-1 4053933 N/A 4053942 unspecified role unspecified role bait bait 55nt-vrna (5' region) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4053940 unspecified role unspecified role prey prey purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3C, 6F, Supp. figure 3B Deletion of any domain disrupted the RNA-binding ability of N protein. GCG (-)-gallocatechin gallate (GCG), a polyphenol from green tea, CHEBI:156271. 55_nt_vrna-n-2 4053933 N/A 4053942 unspecified role unspecified role bait bait 55nt-vrna (5' region) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4053940 unspecified role unspecified role prey prey purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053950 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 6F GCG (-)-gallocatechin gallate (GCG), a polyphenol from green tea, CHEBI:156271. n-n-62 4053934 N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 4053940 unspecified role unspecified role neutral component neutral component purified purified C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1 F The photo-bleached fluorescence signal of N-RNA droplets can be recovered within seconds. n-n-66 4053935 N/A 4053940 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4053940 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 2 E poly(I:C) -CHEBI:84491 n-n-65 4053936 N/A 4053940 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4053940 unspecified role unspecified role neutral component neutral component C-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 2 D poly(I:C) -CHEBI:84491 vRNA 3′ UTR (NC_045512.2 residues 29675-29903). Transfection of N-expressing cells with poly(I:C) or the vRNA (3′ UTR), resulted in the formation of N protein condensates. gallocatechin gallate-n-1 4053937 N/A 4053940 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 9inse 9inse N/A 4053950 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 6G gallocatechin gallate-n-2 4053938 N/A 4053940 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma N/A 4053950 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false 6H