MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url&gt;http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint may-2019-3 human-lnz308 Human glioblastoma cell line. glioma Glioma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot May JL., Kouri FM., Hurley LA., Liu J., Tommasini-Ghelfi S., Ji Y., Gao P., Calvert AE., Lee A., Chandel NS., Davuluri RV., Horbinski CM., Locasale JW., Stegh AH. Brain Disease - Neurological Diseases & Disorders a-stegh@northwestern.edu <a-stegh@northwestern.edu>; Science advances 2019 Only protein-protein interactions imex curation imex curation may-2019-1 human-u87 Human U-87 MG glioblastoma cell line u87 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot May JL., Kouri FM., Hurley LA., Liu J., Tommasini-Ghelfi S., Ji Y., Gao P., Calvert AE., Lee A., Chandel NS., Davuluri RV., Horbinski CM., Locasale JW., Stegh AH. Brain Disease - Neurological Diseases & Disorders a-stegh@northwestern.edu <a-stegh@northwestern.edu>; Science advances 2019 Only protein-protein interactions imex curation imex curation may-2019-2 human-astrocyte Homo sapiens astrocyte astrocyte Astrocytes [Brain astrocytes; Astroglia] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot May JL., Kouri FM., Hurley LA., Liu J., Tommasini-Ghelfi S., Ji Y., Gao P., Calvert AE., Lee A., Chandel NS., Davuluri RV., Horbinski CM., Locasale JW., Stegh AH. Brain Disease - Neurological Diseases & Disorders a-stegh@northwestern.edu <a-stegh@northwestern.edu>; Science advances 2019 Only protein-protein interactions imex curation imex curation may-2019-4 human-gic human glioma-initiating cells gic glioma-initiating cells patient-derived glioma-initiating cells anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag May JL., Kouri FM., Hurley LA., Liu J., Tommasini-Ghelfi S., Ji Y., Gao P., Calvert AE., Lee A., Chandel NS., Davuluri RV., Horbinski CM., Locasale JW., Stegh AH. Brain Disease - Neurological Diseases & Disorders a-stegh@northwestern.edu <a-stegh@northwestern.edu>; Science advances 2019 Only protein-protein interactions imex curation imex curation may-2019-5 human-astrocyte Homo sapiens astrocyte astrocyte Astrocytes [Brain astrocytes; Astroglia] confocal microscopy confocal microscopy immunostaining immunostaining May JL., Kouri FM., Hurley LA., Liu J., Tommasini-Ghelfi S., Ji Y., Gao P., Calvert AE., Lee A., Chandel NS., Davuluri RV., Horbinski CM., Locasale JW., Stegh AH. Brain Disease - Neurological Diseases & Disorders a-stegh@northwestern.edu <a-stegh@northwestern.edu>; Science advances 2019 Only protein-protein interactions imex curation imex curation glyc_human Serine hydroxymethyltransferase, cytosolic Glycine hydroxymethyltransferase SHMT1 Serine methylase protein protein human Homo sapiens Human MTMPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF idh3a_human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial IDH3A Isocitric dehydrogenase subunit alpha NAD(+)-specific ICDH subunit alpha protein protein human Homo sapiens Human MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD idh3a-shmt1-3 3980928 N/A 3980933 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3980934 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false fig. 4f Interaction identified also in LNZ308 Human glioblastoma cell line idh3a-shmt1-4 3980928 N/A 3980934 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3980933 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false fig. 4f Interaction identified also in LNZ308 Human glioblastoma cell line idh3a-shmt1-1 3980929 N/A 3980933 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3980934 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false fig. 4f Interaction identified also in LNZ308 Human glioblastoma cell line idh3a-shmt1-2 3980930 N/A 3980933 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3980934 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false fig. 4f Interaction identified also in LNZ308 Human glioblastoma cell line idh3a-shmt1-5 3980931 N/A 3980934 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3980933 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false The IDH3a interactome was defined using IP-MS studies in patient-derived GICs, expressing a scrambled or an IDH3a- targeting shRNA. Peptides derived from four proteins were reproducibly detected in shScr-expressing GICs upon IDH3a-specific pulldown, but not in immunoglobulin G (IgG) isotype-matched control precipitates or in GICs stably expressing shRNA targeted to IDH3a (Fig. 4, D and E). Among the four candidates, cSHMT was the only IDH3a interactor that coprecipitated with IDH3a in all transformed GBM cell lines, patient-derived GICs, and NHAs. fig. 4d 1-178 3980932 N/A 3980934 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3980933 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false suppl. fig 3a-b,d confocal IF microscopy analysis confirmed colocalization of IDH3α and cSHMT in the cytosol (fig. S3A) and at the nuclear envelope in S phase–arrested cells (fig. S3B).