IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhao-2015-20 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation zhao-2015-15 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. H1299 P53 KO cells were used in this experiment. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation zhao-2015-16 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation zhao-2015-17 mouse-thymic lymphoma Mouse thymic lymphoma thymic lymphoma Thymic lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation zhao-2015-18 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation zhao-2015-19 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation zhao-2015-21 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhao Y., Zhang C., Yue X., Li X., Liu J., Yu H., Belyi VA., Yang Q., Feng Z., Hu W. H1299 P53 KO cells were used in this experiment. wh221@cinj.rutgers.edu, fengzh@cinj.rutgers.edu Cell death and differentiation 2015 Only protein-protein interactions imex curation imex curation ruvb1_human RuvB-like 1 RUVBL1 NMP238 TIP49 TIP49A 49 kDa TATA box-binding protein-interacting protein TIP49a Pontin 52 Nuclear matrix protein 238 54 kDa erythrocyte cytosolic protein INO80H TIP60-associated protein 54-alpha INO80 complex subunit H protein protein human Homo sapiens Human MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK stmn1_human_gene Stathmin STMN1 C1orf215 LAP18 OP18 gene gene human Homo sapiens Human p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD ripk1_human_gene Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP RIP1 gene gene human Homo sapiens Human itpr1_human_gene ITPR1 gene gene human Homo sapiens Human ruvb1_mouse RuvB-like 1 Ruvbl1 Tip49 Tip49a 49 kDa TATA box-binding protein-interacting protein TIP49a Pontin 52 DNA helicase p50 protein protein mouse Mus musculus Mouse MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGVAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD stmn1-stmn1-1 3977565 N/A 3977572 unspecified role unspecified role ancillary ancillary physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3977573 unspecified role unspecified role prey prey SREBP-binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 1:25906409-25906736 Primers used: F: 5′-GAGAATGGGGAGCTGGTTCG-3′ (1:25906717-25906736:-) R: 5′-GACCACACTCTGAGCACCAA-3′ (1:25906409-25906428:+)(1:25906409-25906428:+) N/A 3977574 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5e tp53-ripk1-1 3977565 N/A 3977572 unspecified role unspecified role ancillary ancillary physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3977575 unspecified role unspecified role prey prey NF-κB-binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 6:3064454-3064791 Primers used: F: 5′-ATCCGAGCACTCTTACCCATTCTA-3′ (6:3064454-3064477:+) R: 5′-GTCACCTCTTCGCAAACTCCTG-3′ (6:3064770-3064791:-) N/A 3977574 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5e tp53-itpr1-1 3977565 N/A 3977572 unspecified role unspecified role ancillary ancillary physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3977576 unspecified role unspecified role prey prey NF-κB-binding site sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3:4493661-4493986 Primers used: F: 5′-CAGAAGTTTTTGCCCCGCTC-3′ (3:4493661-4493680:+) R: 5′-GGAGCGCAAGAGAGGGATAC-3′ (3:4493967-4493986:-) N/A 3977574 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed engineered engineered P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5e ruvbl1-tp53-1 3977566 N/A 3977574 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P4 fragment required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false P04637:p.Arg248Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P04637:p.Arg273His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P04637:p.Arg175His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P3 fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3977572 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1a, b, d ruvbl1-tp53-5 3977566 N/A 3977574 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 3977572 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9Y265:p.Asp302Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4a, 3a, b tp53-ruvbl1-1 3977567 N/A 3977574 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 3977572 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 1c ruvbl1-tp53-3 3977567 N/A 3977574 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P04637:p.Arg175His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false N/A 3977572 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 1c ruvbl1-tp53-4 3977568 N/A 3977577 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3977578 unspecified role unspecified role bait bait engineered engineered P02340:p.Arg172His mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1e This interaction is only found in tumour tissue, not in healthy thymus. ruvbl1-tp53-6 3977569 N/A 3977574 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P04637:p.Arg175His mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false N/A 3977572 anti tag western anti tag western blot anti tag western unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 5a tp53-ruvbl1-2 3977570 N/A 3977574 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed P04637:p.Arg175His mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false N/A 3977572 anti tag western anti tag western blot anti tag western unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 5a ruvbl1-tp53-2 3977571 N/A 3977574 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P04637:p.Arg175His mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false N/A 3977572 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1a