UniProt UniProt http://www.uniprot.org zhang-2020-8 human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang S., Liu Y., Zhang B., Zhou J., Li T., Liu Z., Li Y., Yang M. maojunyang@tsinghua.edu.cn Science advances 2020 Only protein-protein interactions imex curation imex curation zhang-2020-7 human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Zhang S., Liu Y., Zhang B., Zhou J., Li T., Liu Z., Li Y., Yang M. maojunyang@tsinghua.edu.cn Science advances 2020 Only protein-protein interactions imex curation imex curation clcn7_human H(+)/Cl(-) exchange transporter 7 CLCN7 Chloride channel protein 7 Chloride channel 7 alpha subunit protein protein human Homo sapiens Human MANVSKKVSWSGRDRDDEEAAPLLRRTARPGGGTPLLNGAGPGAARQSPRSALFRVGHMSSVELDDELLDPDMDPPHPFPKEIPHNEKLLSLKYESLDYDNSENQLFLEEERRINHTAFRTVEIKRWVICALIGILTGLVACFIDIVVENLAGLKYRVIKGNIDKFTEKGGLSFSLLLWATLNAAFVLVGSVIVAFIEPVAAGSGIPQIKCFLNGVKIPHVVRLKTLVIKVSGVILSVVGGLAVGKEGPMIHSGSVIAAGISQGRSTSLKRDFKIFEYFRRDTEKRDFVSAGAAAGVSAAFGAPVGGVLFSLEEGASFWNQFLTWRIFFASMISTFTLNFVLSIYHGNMWDLSSPGLINFGRFDSEKMAYTIHEIPVFIAMGVVGGVLGAVFNALNYWLTMFRIRYIHRPCLQVIEAVLVAAVTATVAFVLIYSSRDCQPLQGGSMSYPLQLFCADGEYNSMAAAFFNTPEKSVVSLFHDPPGSYNPLTLGLFTLVYFFLACWTYGLTVSAGVFIPSLLIGAAWGRLFGISLSYLTGAAIWADPGKYALMGAAAQLGGIVRMTLSLTVIMMEATSNVTYGFPIMLVLMTAKIVGDVFIEGLYDMHIQLQSVPFLHWEAPVTSHSLTAREVMSTPVTCLRRREKVGVIVDVLSDTASNHNGFPVVEHADDTQPARLQGLILRSQLIVLLKHKVFVERSNLGLVQRRLRLKDFRDAYPRFPPIQSIHVSQDERECTMDLSEFMNPSPYTVPQEASLPRVFKLFRALGLRHLVVVDNRNQVVGLVTRKDLARYRLGKRGLEELSLAQT ostm1_human Osteopetrosis-associated transmembrane protein 1 OSTM1 Chloride channel 7 beta subunit GL HSPC019 UNQ6098/PRO21201 protein protein human Homo sapiens Human MEPGPTAAQRRCSLPPWLPLGLLLWSGLALGALPFGSSPHRVFHDLLSEQQLLEVEDLSLSLLQGGGLGPLSLPPDLPDLDPECRELLLDFANSSAELTGCLVRSARPVRLCQTCYPLFQQVVSKMDNISRAAGNTSESQSCARSLLMADRMQIVVILSEFFNTTWQEANCANCLTNNSEELSNSTVYFLNLFNHTLTCFEHNLQGNAHSLLQTKNYSEVCKNCREAYKTLSSLYSEMQKMNELENKAEPGTHLCIDVEDAMNITRKLWSRTFNCSVPCSDTVPVIAVSVFILFLPVVFYLSSFLHSEQKKRKLILPKRLKSSTSFANIQENSN clcn7-ostm1-2 3948313 N/A 3948315 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3948316 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q86WC4:p.Tyr300Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig.2E The CLC-7 E416A mutation resulted in a remarkably reduced protein level compared with wild-type (WT). ostm1-clcn7-7 3948313 N/A 3948315 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminal strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3948316 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q86WC4:p.Tyr300Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig.2E The CLC-7 E416A mutation resulted in a remarkably reduced protein level compared with wild-type (WT). clcn7-ostm1-4 3948314 N/A 3948315 unspecified role unspecified role neutral component neutral component purified purified c-terminal strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 3948316 unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Table 1