IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ye-2020-15 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ye Q, West AMV, Silletti S, Corbett KD Virus - Publications including interactions involving viral proteins kcorbett@ucsd.edu 2020 Only protein-protein interactions imex curation imex curation Ye Q., West AMV., Silletti S., Corbett KD. Coronavirus - Interactions investigated in the context of Coronavirus Protein science : a publication of the Protein Society ye-2020-14 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ye Q, West AMV, Silletti S, Corbett KD Virus - Publications including interactions involving viral proteins kcorbett@ucsd.edu 2020 Only protein-protein interactions imex curation imex curation Ye Q., West AMV., Silletti S., Corbett KD. Coronavirus - Interactions investigated in the context of Coronavirus Protein science : a publication of the Protein Society ye-2020-16 in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Ye Q, West AMV, Silletti S, Corbett KD Virus - Publications including interactions involving viral proteins kcorbett@ucsd.edu 2020 Only protein-protein interactions imex curation imex curation Ye Q., West AMV., Silletti S., Corbett KD. Coronavirus - Interactions investigated in the context of Coronavirus Protein science : a publication of the Protein Society ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA n-38 3712907 N/A 3712910 unspecified role unspecified role neutral component neutral component purified purified N/A 3712910 unspecified role unspecified role neutral component neutral component partially purified partially purified physical association physical association false false false Figure 3A N (FL) formed a large oligomer that eluted in the void volume. (M.Wt >670 kDa). n-39 3712907 N/A 3712910 unspecified role unspecified role neutral component neutral component partially purified partially purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false Figure 3D n-40 3712907 N/A 3712910 unspecified role unspecified role neutral component neutral component partially purified partially purified 2b3_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 physical association physical association false false false Figure 3E n-37 3712908 N/A 3712910 unspecified role unspecified role neutral component neutral component purified purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Table S1 1.42A Rwork=0.157 (0.253) RFree=0.173 (0.243) n-36 3712908 N/A 3712910 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false Table S1 1.45A Rwork=0.167 (0.288) RFree=0.180 (0.310) n-41 3712909 N/A 3712910 unspecified role unspecified role neutral component neutral component purified purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false possible binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3712910 unspecified role unspecified role neutral component neutral component purified purified 2b_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false possible binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 4