MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint yang-2020-3 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn EMBO reports 2020 Only protein-protein interactions imex curation imex curation yang-2020-4 mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. nTregs (CD4+CD25high) isolated by FACS were activated by anti-CD3 and anti-CD28 (1 and 4 lg/ml,respectively) overnight in the presence of IL-2 (100 U/ml). Tregs were generated as in Fig 1 from naïve CD4+T cells FACS isolated from pooled suspensions of thelymph node and spleen cells of wild-type C57BL/6 mice (n = 2–3/experiment). allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn EMBO reports 2020 Only protein-protein interactions imex curation imex curation yang-2020-5 mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes confocal microscopy confocal microscopy immunostaining immunostaining Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn EMBO reports 2020 Only protein-protein interactions imex curation imex curation yang-2020-6 human-jurkat Homo sapiens jurkat Human T cell leukemia confocal microscopy confocal microscopy immunostaining immunostaining Yang J., Wei P., Barbi J., Huang Q., Yang E., Bai Y., Nie J., Gao Y., Tao J., Lu Y., Xie C., Hou X., Ren J., Wu X., Meng J., Zhang Y., Fu J., Kou W., Gao Y., Chen Z., Liang R., Tsun A., Li D., Guo W., Zhang S., Zheng SG., Niu J., Galardy P., Tong X., Shi G., Li H., Pan F., Li B. allergyli@163.com; fan.pan@siat.ac.cn; binli@shsmu.edu.cn EMBO reports 2020 Only protein-protein interactions imex curation imex curation foxp3_human Forkhead box protein P3 Scurfin FOXP3 IPEX JM2 protein protein human Homo sapiens Human MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP ubp44_human Ubiquitin carboxyl-terminal hydrolase 44 USP44 Ubiquitin-specific-processing protease 44 Deubiquitinating enzyme 44 Ubiquitin thioesterase 44 protein protein human Homo sapiens Human MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSCSHVACGRYIEEHALKHFQESSHPVALEVNEMYVFCYLCDDYVLNDNTTGDLKLLRRTLSAIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHRRRILMGKIFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPPRKSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKVSDSSVKRRPIVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQMNECQEKDTGFVCSRQSSLSSGLSGGASKGRKMELIQPKEPTSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTSLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIEPFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFSSKPVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNMEPYCCRETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMDEVCKAQAYILFYTQRVTENGHSKLLPPELLLGSQHPNEDADTSSNEILS foxp3_mouse Forkhead box protein P3 Foxp3 Scurfin protein protein mouse Mus musculus Mouse MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKRSQRPNKCSNPCP ubp44_mouse Ubiquitin carboxyl-terminal hydrolase 44 Deubiquitinating enzyme 44 Ubiquitin thioesterase 44 Ubiquitin-specific-processing protease 44 Usp44 protein protein mouse Mus musculus Mouse MDRCKHVEQLQLAQGHSILDPQKWYCMVCNTTESIWACLSCSHVACGKYIQEHALKHFQESSHPVAFEVNDMYAFCYLCNDYVLNDNAAGDLKSLRSTLSTIKSKKYPCVVPSDSVLHPVDAQDRVYSLLDGTQSLPGNEDPTCAALWHRRRVLMGKAFRTWFEQSAIGRKGQEPTQERMVAKREAKRRQQQELEQQMKAELESTPPRKSLRLQGSSEEAATIEIVPVRAPPPPPASPAKDKAALPTSEDRTFKKVSDSLIKRRPMVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKLDLNQWLAVAASDKARSYKHSAVTEAAAQQMNEGQEKEKGFVCSRHSGLSSGLSGGASKGRNMELIQPREPSSPYSSLCHELHILFQVMWSGEWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQRELETTGTKLPALIPTSQRRLIEQVLNVVNNIFHGQFLSQVTCLACDNKSDTIESFWDLSLEFPERYQCSGKDAASQPCLVTDMLDKFTETEALEGKIYMCDHCNSKRRKFSSKSVVFTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVVFEETLNMEPYCCRETLNALRPECFLYNLSAVVIHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMEEVRKAQAYILFYTQRVTENGHSKLLPPELLSNSQHPSKETDASSNEVLS foxp3-usp44-1 3931419 N/A 3931423 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3931424 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false fig.2a left foxp3-usp44-2 3931419 N/A 3931423 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3931424 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false fig.2a right foxp3-usp44-4 3931420 N/A 3931425 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3931426 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false fig.2b-c (left) Specifically, immunoprecipitation ofFOXP3 from the lysate of murine iTregs or nTregs readily pulleddown USP44, and this DUB was similarly able to coimmunoprecipi-tate FOXP3 in reciprocal experiments (Fig 2B and C). foxp3-usp44-3 3931420 N/A 3931425 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3931426 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false fig.2b-c (right) Specifically, immunoprecipitation ofFOXP3 from the lysate of murine iTregs or nTregs readily pulleddown USP44, and this DUB was similarly able to coimmunoprecipi-tate FOXP3 in reciprocal experiments (Fig 2B and C). 1-164 3931421 N/A 3931426 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3931425 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false fig. 2d Confocal microscopy of primary murine Tregs confirmed the colocalization of FOXP3 and USP44 in wild‐type‐derived cells but not in cells isolated from mice lacking USP44 expression (Usp44fl/flFoxp3Cre+; Fig 2D). 1-165 3931422 N/A 3931424 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3931423 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false EV4