IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-2020-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kim. J Coronavirus - Interactions investigated in the context of Coronavirus andrzejj@anl.gov 2020 Only protein-protein interactions imex curation imex curation Kim. Y, Kim Y., Jedrzejczak R., Maltseva NI., Wilamowski M., Endres M., Godzik A., Michalska K., Joachimiak A. Protein science : a publication of the Protein Society kim-2020-3 in vitro In vitro ribonuclease assay ribonuclease assay predetermined predetermined participant predetermined Kim. Y, Kim Y., Jedrzejczak R., Maltseva NI., Wilamowski M., Endres M., Godzik A., Michalska K., Joachimiak A. Coronavirus - Interactions investigated in the context of Coronavirus An increase in fluorescence is detected when the FAM group is cleaved from the TAMRA quenched peptide. andrzejj@anl.gov Protein science : a publication of the Protein Society 2020 Only protein-protein interactions imex curation imex curation p0dtd1-pro_0000449632 Uridylate-specific endoribonuclease nsp15 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQ 6453 6798 dna_rna_oligo dArUdAdA nucleic acid nucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) AUAA nsp15_wcpv-1 3682967 N/A 3682969 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Table 1 dna_rna_oligo-nsp15_wcpv-1 3682968 N/A 3682970 enzyme target enzyme target substrate substrate neutral component neutral component 5' FAM fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain false TAMRA tetramethyl rhodamine label tetramethyl rhodamine label certain certain sequence position certain certain certain sequence position certain false N/A 3682969 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) rna cleavage rna cleavage false false false Figure 6 A-C