MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint crown-2019-1 in vitro In vitro aggregation aggregation assay aggregation predetermined predetermined participant predetermined Crown A., McAlary L., Fagerli E., Brown H., Yerbury JJ., Galaleldeen A., Cashman NR., Borchelt DR., Ayers JI. W32S mutation was remapped to position 33 in agreement with Uniprot numeration. Jacob.Ayers@ucsf.edu PloS one 2019 imex curation Only protein-protein interactions imex curation crown-2019-2 in vitro In vitro filter trap assay filter trap assay Filter retention assay Filter retention assay predetermined predetermined participant predetermined Crown A., McAlary L., Fagerli E., Brown H., Yerbury JJ., Galaleldeen A., Cashman NR., Borchelt DR., Ayers JI. W32S mutation was remapped to position 33 in agreement with Uniprot numeration. Jacob.Ayers@ucsf.edu PloS one 2019 imex curation Only protein-protein interactions imex curation sodc_human Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 SOD1 protein protein human Homo sapiens Human MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ sod1-fibrils-1 3947004 N/A 3947006 unspecified role unspecified role neutral component neutral component purified purified P00441:p.Trp33Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3947006 unspecified role unspecified role neutral component neutral component purified purified P00441:p.Trp33Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. S1 we prepared recombinant WT-SOD1 and W32S-SOD1 fibrils as described in Methods (S1 Fig). sod1-sod1-3 3947005 N/A 3947006 unspecified role unspecified role neutral component neutral component purified purified P00441:p.Trp33Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3947006 unspecified role unspecified role neutral component neutral component purified purified P00441:p.Trp33Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 1A we assessed the levels of large aggregates by filter trap assay and observed a ~5 fold greater level WT-SOD1 fibrils than W32S-SOD1 fibrils in our preparations (Fig 1a).