IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nishimiya-2019-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Nishimiya D., Kawaguchi Y., Kodama S., Nasu H., Yano H., Yamaguchi A., Tamura M., Hashimoto R. nishimiya.daisuke.tc@daiichisankyo.co.jp Scientific reports 2019 Only protein-protein interactions imex curation imex curation nishimiya-2019-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nishimiya D., Kawaguchi Y., Kodama S., Nasu H., Yano H., Yamaguchi A., Tamura M., Hashimoto R. nishimiya.daisuke.tc@daiichisankyo.co.jp Scientific reports 2019 Only protein-protein interactions imex curation imex curation klk4_human Kallikrein-4 KLK4 EMSP1 Enamel matrix serine proteinase 1 Kallikrein-like protein 1 PRSS17 PSTS Prostase Serine protease 17 protein protein human Homo sapiens Human MATAGNPWGWFLGYLILGVAGSLVSGSCSQIINGEDCSPHSQPWQAALVMENELFCSGVLVHPQWVLSAAHCFQNSYTIGLGLHSLEADQEPGSQMVEASLSVRHPEYNRPLLANDLMLIKLDESVSESDTIRSISIASQCPTAGNSCLVSGWGLLANGRMPTVLQCVNVSVVSEEVCSKLYDPLYHPSMFCAGGGHDQKDSCNGDSGGPLICNGYLQGLVSFGKAPCGQVGVPGVYTNLCKFTEWIEKTVQAS isk2_human Serine protease inhibitor Kazal-type 2 SPINK2 Acrosin-trypsin inhibitor Epididymis tissue protein Li 172 HUSI-II protein protein human Homo sapiens Human MALSVLRLALLLLAVTFAASLIPQFGLFSKYRTPNCSQYRLPGCPRHFNPVCGSDMSTYANECTLCMKIREGGHNIKIIRNGPC klk4-spink2-1 3682409 N/A 3682411 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3682412 unspecified role unspecified role prey prey purified purified P20155:p.[Ser37_Gln38delinsArgLys;Arg40_Gly43delinsGluTyrGlyVal;Pro45Gln;His47_Asn49delinsThrTyrLeu] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3682409 3682409 3682409 Table 3, Suppl. Figure 4 klk4-spink2-3 3682409 N/A 3682411 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3682412 unspecified role unspecified role prey prey purified purified P20155:p.[Ser37_Gln38delinsGluLeu;Arg40_Gly43delinsValGluAspVal;Pro45Gln;His47Ile;Asn49Lys] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3682409 3682409 3682409 Table 3, Suppl. Figure 4 klk4-spink2-4 3682409 N/A 3682411 unspecified role unspecified role bait bait purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f N/A 3682412 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P20155:p.[Ser37_Gln38delinsArgArg;Arg40_Pro42delinsSerIleHis;Pro45Asn;His47_Asn49delinsMetTyrAla] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3682409 3682409 3682409 Table 3, Suppl. Figure 4 klk4-spink2-2 3682410 N/A 3682412 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P20155:p.[Ser37_Gln38delinsArgArg;Arg40_Pro42delinsSerIleHis;Pro45Asn;His47_Asn49delinsMetTyrAla] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3682411 unspecified role unspecified role neutral component neutral component purified purified human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction false false false Fig 4, Suppl. Table 3 working 18.3%, free 22.6% 2.0A