IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ brown-2018-1 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Brown CY., Dayan S., Wong SW., Kaczmarek A., Hope CM., Pederson SM., Arnet V., Goodall GJ., Russell D., Sadlon TJ., Barry SC. simon.barry@adelaide.edu.au Oncotarget 2018 imex curation Only protein-protein interactions imex curation brown-2018-2 human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Brown CY., Dayan S., Wong SW., Kaczmarek A., Hope CM., Pederson SM., Arnet V., Goodall GJ., Russell D., Sadlon TJ., Barry SC. simon.barry@adelaide.edu.au Oncotarget 2018 imex curation Only protein-protein interactions imex curation hsa-mir-155-5p microrna hsa-miR-155 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UUAAUGCUAAUCGUGAUAGGGGU mrna_zeb2 zinc finger E-box binding homeobox 2 mrna messenger rna mRNA mrna human Homo sapiens Human AGTTCTAACCTGCTCTGCAGGAATAACGGTCCTGCCTCCCGACACTCTTGGCGAGGTTTTTGTACAGTTTGCTCCGGGAGCTGTTTCTTCGCTTCCACCTTTTTCTCCCCCACACTTCGCGGCTTCTTCATGCTTTTTCTTCTCACCATTTCTGGCCAAAACTACAAACAAGACTTCGCAGATCGAGCCTGCGTGCTGCCGAAGCAGGGCGCCGAGTCCATGCGAACTGCCATCTGATCCGCTCTTATCAATGAAGCAGCCGATCATGGCGGATGGCCCCCGGTGCAAGAGGCGCAAACAAGCCAATCCCAGGAGGAAAAACGTGGTGAACTATGACAATGTAGTGGACACAGGTTCTGAAACAGATGAGGAAGACAAGCTTCATATTGCTGAGGATGACGGTATTGCCAACCCTCTGGACCAGGAGACGAGTCCAGCTAGTGTGCCCAACCATGAGTCCTCCCCACACGTGAGCCAAGCTCTGTTGCCAAGAGAGGAAGAGGAAGATGAAATAAGGGAGGGTGGAGTGGAACACCCCTGGCACAACAACGAGATTCTACAAGCCTCTGTAGATGGTCCAGAAGAAATGAAGGAAGACTATGACACTATGGGGCCAGAAGCCACGATCCAGACCGCAATTAACAATGGTACAGTGAAGAATGCAAATTGCACATCAGATTTTGAGGAATACTTTGCCAAAAGAAAACTGGAGGAACGCGATGGTCATGCAGTCAGCATCGAGGAGTACCTTCAGCGCAGTGACACAGCCATTATTTACCCAGAAGCCCCTGAGGAGCTGTCTCGCCTTGGCACGCCAGAGGCCAATGGGCAAGAAGAAAATGACCTGCCACCTGGAACTCCAGATGCTTTTGCCCAACTGCTGACCTGCCCCTACTGCGACCGGGGCTACAAGCGCTTGACATCACTGAAGGAGCACATCAAGTACCGCCACGAGAAGAATGAAGAGAACTTTTCCTGCCCTCTCTGTAGCTACACGTTTGCCTACCGCACCCAGCTCGAGCGGCATATGGTGACACACAAGCCAGGGACAGATCAGCACCAAATGCTAACCCAAGGAGCAGGTAATCGCAAGTTCAAATGCACAGAGTGTGGCAAGGCCTTCAAATATAAACACCATCTGAAAGAACACCTGCGAATTCACAGTGGTGAAAAACCTTACGAGTGCCCAAACTGCAAGAAACGTTTCTCCCATTCTGGTTCCTACAGTTCGCACATCAGCAGCAAGAAATGTATTGGTTTAATCTCTGTAAATGGCCGAATGAGAAACAATATCAAGACGGGTTCTTCCCCTAATTCTGTTTCTTCTTCTCCTACTAATTCAGCCATTACCCAGTTAAGAAACAAGTTGGAGAATGGAAAACCACTTAGTATGTCTGAACAGACAGGCTTACTTAAAATTAAAACAGAACCACTAGACTTCAATGACTATAAAGTTCTTATGGCTACACACGGGTTTAGTGGCACTAGTCCCTTTATGAATGGTGGGCTTGGAGCCACCAGCCCTTTAGGAGTTCATCCATCTGCTCAGAGTCCAATGCAGCACTTAGGTGTAGGGATGGAAGCCCCTTTACTTGGGTTTCCCACCATGAATAGTAATTTAAGTGAGGTACAAAAGGTTCTACAGATTGTGGACAATACTGTTTCCAGGCAAAAAATGGACTGCAAGGCTGAAGAAATTTCAAAGTTGAAAGGTTATCACATGAAGGATCCATGCTCTCAACCTGAGGAACAAGGAGTTACTTCTCCTAATATTCCGCCTGTCGGTCTTCCGGTAGTGAGTCATAATGGTGCCACTAAAAGTATTATTGACTATACGTTGGAAAAAGTCAATGAAGCCAAAGCTTGCCTCCAGAGCTTGACTACTGACTCAAGGAGACAGATCAGTAATATAAAGAAAGAGAAGCTACGTACTTTAATAGATTTGGTCACTGATGACAAAATGATTGAGAACCACAACATATCCACTCCATTTTCATGCCAGTTCTGTAAAGAAAGTTTTCCTGGCCCCATCCCTTTGCATCAGCATGAACGTTACCTTTGTAAGATGAATGAAGAGATCAAGGCGGTCCTGCAGCCTCATGAAAACATAGTCCCCAACAAAGCCGGAGTTTTTGTTGATAATAAAGCCCTCCTCTTGTCATCTGTACTTTCTGAGAAAGGAATGACAAGCCCCATCAACCCATACAAGGACCACATGTCTGTACTCAAAGCATACTATGCTATGAACATGGAGCCCAACTCCGATGAACTGCTGAAAATTTCCATTGCTGTGGGCCTTCCTCAGGAATTTGTGAAGGAATGGTTTGAACAACGAAAAGTCTACCAGTACTCAAATTCCAGGTCCCCATCCCTGGAAAGAAGCTCCAAGCCGTTAGCTCCCAACAGTAACCCTCCCACAAAAGACTCTTTATTACCCAGGTCTCCTGTAAAACCTATGGACTCCATAACATCACCATCTATAGCAGAACTCCACAACAGTGTTACGAATTGTGATCCTCCTCTCAGGCTAACAAAACCTTCCCATTTTACCAATATTAAACCAGTTGAAAAATTGGACCACTCCAGGAGTAATACTCCTTCTCCCTTAAATCTTTCCTCCACATCTTCTAAAAACTCCCACAGTAGTTCATACACTCCAAACAGCTTCTCTTCTGAGGAGCTCCAGGCTGAGCCTTTAGACTTGTCATTACCAAAACAAATGAAAGAACCCAAAAGTATTATAGCCACAAAGAACAAAACAAAAGCTAGTAGCATCAGTTTAGATCATAACAGTGTTTCTTCCTCATCTGAAAACTCAGATGAGCCTCTGAACTTGACTTTTATCAAGAAGGAATTTTCAAATTCAAATAATCTGGACAACAAAAGCACTAACCCAGTGTTCAGCATGAACCCATTTAGTGCCAAACCTTTATACACAGCTCTTCCACCTCAAAGCGCATTTCCCCCTGCTACTTTCATGCCACCAGTCCAGACCAGTATTCCTGGGCTACGACCATACCCAGGACTGGATCAGATGAGCTTCCTACCACATATGGCCTACACCTACCCAACTGGAGCAGCTACTTTTGCTGATATGCAGCAAAGGAGAAAGTACCAGCGGAAACAAGGATTTCAGGGAGAATTGCTTGATGGAGCACAAGACTACATGTCAGGCCTAGATGATATGACAGACTCCGACTCCTGTCTGTCTCGCAAAAAGATCAAGAAGACAGAGAGTGGCATGTATGCATGTGACTTATGTGACAAGACATTCCAGAAAAGCAGTTCCCTTCTGCGACATAAATACGAACACACAGGAAAAAGACCACATCAGTGTCAGATTTGTAAGAAAGCGTTTAAACACAAGCACCACCTTATCGAGCACTCAAGGCTTCACTCGGGCGAGAAGCCCTATCAGTGTGATAAATGTGGCAAGCGCTTCTCACACTCGGGCTCGTACTCGCAGCACATGAATCACAGGTATTCCTACTGCAAGCGGGAGGCGGAGGAGCGGGAAGCGGCGGAGCGCGAGGCGCGCGAGAAAGGGCACTTGGAACCCACCGAGCTGCTGATGAACCGGGCTTACTTGCAGAGCATTACCCCTCAGGGGTACTCTGACTCGGAGGAGAGGGAGAGTATGCCGAGGGATGGCGAGAGCGAGAAGGAGCACGAGAAAGAAGGCGAGGATGGCTACGGGAAGCTGGGCAGACAGGATGGCGACGAGGAGTTCGAGGAGGAAGAGGAAGAAAGTGAAAATAAAAGTATGGATACGGATCCCGAAACGATACGAGATGAAGAAGAGACTGGAGATCACTCCATGGACGATAGTTCGGAGGATGGGAAAATGGAAACCAAATCAGACCACGAGGAAGACAATATGGAAGATGGCATGTAATAAACTACTGCATTTTAAGCTTCCTATTTTTTTTTCCAGTAGTATTGTTACCTGCTTGAAAACACTGCTGTGTTAAGCTGTTCATGCACGTGCCTGACGCTTCCAGGAAGCTGTAGAGAGGGACAGAAGGGGCGGTTCAGCCAAGACAGATGTAGACGGAGTTGGAGCTGGGTATTGTTAAAAACTGCATTATGCAAAAATTTTGTACAGTGTTAAGGCCTAAAAACTGTGTGGTTCAGAGACTAATTCCTGTGTTTAATAGCATTTATACTTTAAGCACAACTAGAAAATTGTAAGAATTGCACTCTACTTATGTATCACTACAAACTTTAAAAAACTATGTCTAATTTATATTAATACATTTTAAAAAGGTGCCCGCACTACCATACATCAGTATTTTTATTATTATTATTGTTATTCCTTTTTAATTTAATGTGCTCGCACTACAATGCATCAGTATTATGATTCCTCTGTACTTTCCTTTCGCTATTCATCAATTTCCCATTTTTTTTTTCAGCTTAAGTAACCACACAATTTTAGGCCTCAATTTTTTTTTTTTTCTGTGAAGGAACTTGAAGTGATGCATGTGTGAATTTAAGATACCGAAGTCTTAAAGTGACCTGGACGTGAAGGAAAAAGTAAGATGAGAAATAAAGAAAGCCTTTGTAAGGTGGTTTTAAAAGCCTTATATGCAAACCTTTTAATCTGTGTTTCTGCAAGTGCCATCCTTGTACAGTGTTAAGAGGGTAACATGGGTTACCTTTGCACCAGCTTCAGTGTTAAGCTCACCCTGTTCTTTGAAGCACCCATGTCAGTATTAGAAGAATAGGCAGCAGTTCCTTAGTTTACATATGTTTGTGCAATTATTTTCTGTACTTTTTTGTTCATTAATTTTGTCAGTATTACACCAAACTGTTTTTGCAACAAAAAAATTTTTTTTGCATTCATTTAATTTTAGGTCAAATAACATTTTATTTATGTGGCTCATTTTATATTTCCTAATTTTATTTATTTCATACTGTAGTGTACAGTATTATAGTTCTTCAATATATAGATATATTTTAGTAAAAAAGGAACATGACGTTGATCATTTGGGCAAATTTTACGTAAAGAGAAGAGCATTTATTGTGTTTTGGAACATTAATTGTGAGATGGGATTTTTCAATTTTATTATTTTATTTTTGTTTTTTTCCAATTACTGGAAATTCCAAATTTGGGAACTTTTGATACGATCTTGTGAAAACACTGTATTTTCGACTGAAAATTCCACTTTCTTCATCTTGTTTTTTAGCTAAAAAGAGGGACTGTTAAATACAATGTATGATACCATGACAAAAATCTTTCCTGAATTGTCTTTGTAAAAGTATTATTGAATTTTCAATTTGTAATTTCTTTTGAAAATGACCATGCTCGAATAAAAATGTAGCCAAACTAAGAATGTAGTTAATGAGTTCTGTACTTTTAGAGAGTTTTCCTTCAATGACCATTAACATGTAACATGCTTTATGCTTATAATAATGCTAATTATGTTTTTTTCATATAATTTTAGTTTAGCAATAATTTTGACTGGTACCAATAACTGTTTTTTAAAATTCCATACCTATGTACAGCAATTTTACAGCTTTTCTCAACTGATCCTGATTCCAGATTGTGTATTTTTATGTGAGGTTATATTATTCAAATTTAGTCTATTTACTTTACAGACATTTCTACTTTTGCATTACGAGTATTTAGAGATTATGTGTTAAAAATTCACTTCTCTGTCCAAGGGGTCTTTGTGATTTATTCAAAAAAAAGTCTAATTTCAAAAAGACAGCTATTATTCAGTGTTATTTATAATATGTAACCTTTTTTAAAGGATTGGGATAGTTTATCTCACTTTTTGAAATGCAGACAGTAGTTTACCGTTTATCTGAAACTAGAAGGCGTGGGTGGGAGAGGAAAAGCTAAAAGCAAATGCTAACAAAAATAACCGTGATTTTCTAAGACAGTTTTTCAGTTTTTACAAGATGACCCTAATATTCAGAATATGAATGTATTCGTAGGTTTTACATAATGACTTTTATCAAGAAACTAGATTCTGCTTCTTAAATCTAATTGCCAAGTGAAGAATAACAGAAAAAACAGATTACCTTATCAAATTTACAGCTCTTGAATATACAGAACTATAATATAGTAGCTGTCCATGTATTTTTTCTACTTTAGAATCAAAGAAGAAAAGCATCATTTTGCTATTAAATTTGCTAAAATTTTGAGTATGATATTTCCAGTTGGCAAGAACAACATATTTATATTTATTCCTTAGCCATAATACCACTTTCCTAAATTTCACAAAAGTCATTCTTTGCAACTTGAAACTCAATAGAAAGTGTGTATGTGTGTGTGTGTATATATATATATATATACACACACACACATACACAGAAAGGATGTAATGAAGATACAGTAATAGTTGAGCAGACCTTTTTAGAAAAACATGTTTTTAGCTCTATCTTCAAACTTTCTGGCAGAGGGGGTGGGGGGGGCAGGGGGAGGAGTGGCATCAAAATGCTATGCCTCCTGTTATCCACAGCCTAGAGTTTTTATATTTGGAAAGTTTAGAAAATTCTATCCTCGTTTCTCCTTCTTTGAATGGCACAAATAAATACACTACATAAATTTTTCTGGTTTGAAAGGCTCTAGGCGATAACTTTATTAATTCAACCTGAAAATATCAAGCCATTAAATTTTGTCCGGGTAGAATAAATCCCTGTGGCCTCTTTTAAAGCAATGTAGGTCTCTGTTGCCCATGGGGCATATCTGTGTCCCAATCCACAAGAGATAGGACCAACAAACAATGAATGTGCAACCTAACTCTTTCTCCTTGGAAAGAAGAAAGTGTGCACGAAGTAGAGGAGGGTGGGCAGACCCTGCCTTGCCCCTCCTGTTACCCCCTTCTCTGTCATTTGTTCCTAACTCCATTTCATAGGCAGGCTCAGAATACCTGAGTCTGAAAATATCAGGATAACACTTGTGAATTGTGACAATCACTACAATGTCCCATATCTGAGGAGTTTTTTTTAATGCTATTTATCCGCTGGACACGATTGCACATTAGGGCTGCATAATCCTCTAACTCTAGGGAAAAATAAAAACTTTTGATTTGTCTTAAGATTCTTCTCCAAGGTCGCAAACAAGAAATTCCCCTCCACAACCAAGAGATGTGCATTTTAGTAACATCAGATGTGTTCTTCTGTTTTATCAACTACTTACTCTTCCCACACGCTTAGTTCTAAATCTAACCTTTCCCCCCTCGAATAGGGGGCAGGGGAGGATGAGGAAACACTGGAACAACTGAACACCCCTGCCCATTTTCTCCAAGAGCCTTTTGTATTCTAGCATATCTGTGCAATCTTTTCTTTTTTCTTCACATGACACTGTAAGCTTAGGCCTGAAATAACTGGGAAGAGAGATGCGTATCAGAATTTCTCCGCAAGAGCTAAACAAAACATACATCTTCCTTAGCATGAATTGGACTGGGGGCGGAGTGGGAGGGCTTGGAGGAAAGGGGAAAGAAGGGACTATATTTGAATAAATATGAATAAATGTATTAGATACTTTTCACAATCAGATAACTTTTAAAAAGGTCATTTTTTATCTTTCTAATAATGTAAGCCTTAATAAAAGCAAATCTTAGTCACAAATTTGAGGAGACTGCCCAATAATAAGTTTACATGTATTTGAACTGAAAAATTGTTAACCATGCTTTTGCTCCAAGATGTGTGAGGCCATTCAGGGGCTGTAGGGCCCTGGATATACACACAAACAAGTGTGTGTATATCTGGAGCCCCACACATTGTAATAAACACAGCTGCATTTATTTGACTATGTGATCCCATGTACATGTAAAAACATTCAAACAAACACACTCAGCGGATTTATTTATTGTGCAATGGGGCAATTATTCAAATAAACATGCTCAATGCAATTATTTGAATCTCACATTGCATGTTCATCAATCATAGCACTAAAAAAAGAGGGGGAAAAAACACCAAAGAATTCACATGGGGAAAAAATATATATATGAAAACCACCTTATTATAGATTTTATAGGGCAGCTGAGGTTATGGCTCCCTTCTTAACTGTAACTCAACTATTCTGTATTCAATGACATTTGTTTCTAATGATTAATTGGTTCACTCACTTGATCATATAATAGCAAACTTTATAAACCTGTATTGTGTAGAGATGTGAAATCTCTATATTTCAAGAGCAGAAGAGTTCTTTCTAGACACCTTACATCAAGGGACACTGGTCCAATTATTATCGCTTATATAAGCACTCCTATAAATTCTGAAAAATTTTATACATGCAACAAAACATTCCTACATTTGAAGACATTAAGAAAAATCACAGGTGACTCATCTGATCATTCTATATATTAATAAATATTATGACATATATGTGAACACATCACAAATCATATTGGTGTACCAAGAGGCAATTTATGCCTCTCTTAAGTATGTACTGACATAACCTAATATACTAAAATGGGAAGGGGCTTTTAGTCACTGAAATATGCATCGTGTAACAAAGATGAAGAAAATACATGGCTTGTGCCCATCATAAAAAAAGATTCAGACTGAAGGCTTAGCTTTGGTTTTTTCAATTAAATTGTTAAACTGTGCACAGTGATTTTTTTTTAGAACTTGAGACATTTGTGATGTTGGCTGTTTAAATCTTTGTTACCTTCGCTGTGAATTGAAATTGTACATATTTAGTAAATCATGCAGACAAAACAAACTTTTTAGACAATATTTTTATTGGAGAGTTTTCTTTTCCTGTATCCATGTTAAAAAAAAAAAAGACCTCCTTTCCCAAAATAAAAATGTCAATACTAAATTTAAAGAAGTATAAAGGAATGATTGCTTCCTTTAGAGCAAAATATTTAAATAAACATGGAGATAATTGGCAACATGTTCTTTTTGGGCTAGTAGGCTGTGTCCAATTTTTTGGGTCTGATGTTTCAGAGGGCCTCTGTTTCAGGGTTGAAGATGATATATTAATCTCGGAATTAAACAAATGCTATTAAATAACAGAAATAAATCCTTCAGTCTTCATTTGTATCTGAAATGTGGAATTAGTCAGAGAAATGGCACCGAGATAATATGATGCTGAATATGCCACAAGCTAAAACTTTCTCTATTAAACGAGTCGCCTACTACCTATAATCTAGCATGACATGTTAAAATGCTCCAAAATACTGAAAATAGAAAATGAACACTACTGTTCCGATTTGGTCTTGAGATTAATATGACAAAGATTCTAATAATTTTAGTCAAAGAAAATGATGAGTAAAAAATACATGAAGATTAAAGTGATGACAAATGA foxp3_human Forkhead box protein P3 Scurfin FOXP3 IPEX JM2 protein protein human Homo sapiens Human MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKRSQRPSRCSNPTPGP zeb2_human_gene Human ZFHX1B gene, promoter gene gene human Homo sapiens Human hsa_mir_155_5p-mrna_zeb2-1 3671475 N/A 3671477 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified N/A 3671478 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered r.4156_4161delinsaagctt mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false r.4082_4087delinstgtaca mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range false physical association physical association false false false Fig 3 C, D, E zeb2_human_gene-1 3671476 N/A 3671479 antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3671480 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell engineered engineered FP3 binding region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 2:144452709-144452840 FP3_BR_F:GTTTTTCCTTCCAGCCTTTTTCCT FP3_BR_R:TCCCCACGTAAATGTTTTGCTTTT physical association physical association false false false Fig1B