DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhang-2017-37 Red alga Griffithsia pacifica 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Zhang J., Ma J., Liu D., Qin S., Sun S., Zhao J., Sui SF. suisf@mail.tsinghua.edu.cn Nature 2017 Only protein-protein interactions imex curation imex curation zhang-2017-38 Red alga Griffithsia pacifica molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang J., Ma J., Liu D., Qin S., Sun S., Zhao J., Sui SF. suisf@mail.tsinghua.edu.cn Nature 2017 Only protein-protein interactions imex curation imex curation a0a291fee7_gripa rpeCg4 protein protein Red alga Griffithsia pacifica MDSPAFAVNGMFSAVKVGNSSFTENKVTAVSKTAPTASVRMVVDPFQRKFQSIGKIGIDYSRPKKLATYKRVGYSVGLDFPNAVSMAGHYSLTDCTRAGGAAKILMKYDEYCAKGMLQVYKRSAVSTGVYTTKCTEATQPGVAYDVRVFNRTAAFRQAQKPVNVRLGEQYAARKACVTLAHNCSREEAQFKNMPMSCATFLAGKMEAMGTCYRTVRPSSKAEDYMAGSVRMQVYQKGNASGVYPVGGCEDGHAKGDADLRRVIALASEYRAAQQGAAAVTGAQYASSKMAIQLYGHSCNHEEGQFCDYPAVAAAMCRY a0a291fec7_gripa rpeCg8 protein protein Red alga Griffithsia pacifica MEPAFVSSFAPKPVITTSLTASSPLSVTARKNAVSTPTMAAYSLDKYAQMSGANAVDTSGASPAASSTWWVAYRDSLKERFNPFRAPANPEVDVGKSKEYFFAQTAYGRILNMVNASRFGKGGDPDELVPPPGAQPADQYMANCIVKQYKAMATPTGVYTTQCTEGVVRGQAEEARNAALSAAFRMKQRSSAQKFGDFCESRRMAVIGAHGCSYEESLLTKFPAAARAYTTASSEAKGNCVRYADGTSPAETYMAACVDKQMKFRSVPMGVYDVLCSDGNTKGVAEYKRVSAMSVRFRSNQMSTLYKMQAKYNNAAYARNYFGHGCSYEENLFNKYPAVSASMRPSTARY a0a291fec5_gripa rpeCg5 protein protein Red alga Griffithsia pacifica MYAFAPNTPFTASKAVVGKTSFTSPLPAQSESRPTAAPTMVLRTVLRSPVPSGAATVYGYVGRGNISVILAKADEYMAKSVRKQYLAKSNPYGTFGVQCTEGSVKFAADFSRIRALNAEFRAKLGSASKKTFDMYENRKNAISNSHGCHHEETQFVGYKGVSSMYNVSKSEASGSCSRYASPETVVEAAMLRFMDIQVKMAANPTGVYNISCNEGAARGQAEDVRVAALNAAFRQGQKSLGKLLDEKYQQKKQGYSFAHGCNYEEGLINKYPALGAAFRSKSYGY a0a291fec1_gripa rpeCf9 protein protein Red alga Griffithsia pacifica MHRKPSLSHNLNHNKPCLQPSFCANHLSRFDLPTSSPRPLASVVVLSAMAFVSAPHSSIRSSNSSFTPTTPARRPVCHNAPVVTPRMTFGQTVKETLESNPRYGNLVKLAAAGGVDLEVSNCTVFAPTNGALSGERYDELLADPVAARNVVLRHILPDQVLTSKAIKGCSFWDSLPGGPLPYEGIGPVVKIAGVRLLNESSDDECDNGTIHVIDGIISTPLAKPTPFAGVFEPSVPMLESRDDIATAVYPPVTPDVRRAFGAASAPSTVGGRKAMGLIKQLPFWMYGPPFNASKQEDFEPISIANPDVSSVDYQLMPPGSVIVQPDEVSAAKMLPVSGMSKHIGKTKRLVEGDGLSDYSRLD a0a291fec0_gripa rpeCg7 protein protein Red alga Griffithsia pacifica MTAPAFTAPISLTTPHAFSARGLRPATTSSAAPTAVPTPRMSAADKYMARTVTRTAKSAAAGFGVYTPQCTEASGGANTAEATRLAVLAADFRLRQAPLGARFADLYETRRAAVIQACNSSAEEGYATSFPSRAAASVAGRAEGLRACSRYFPQKPPVEEYMAACVDRQYKQMRVHGGVYSTLCADGRSAGDADTARIAALGARFRAQHLSKSQQTQMRYNAMSEARMLARGLCTYEEAQFNAYPKMAGMMRYGTGVYAASVRGPELVVGNKSMTVAEQVNGVNAESYWPSSKVRPAVARGTSPWMGLGVVKSYAAMSEAAMAYGIEQQSKPYVPQKYEGWSSGWKPKSSLM a0a291feb9_gripa rpeCg6 protein protein Red alga Griffithsia pacifica MAFITSFTPRNLASRSEFTSTSVSTRRPTLARNTIRALFTPPVDEFMASSVQSQYIQKACPSGVPPIQCIEGVTSDQPYAARTLKRQTELRYHQLPVAVKLRKAYETRRAAVVATHGCSHEEGRVLSYPRMASAMLIGQAEASKACSRYFVPNGPAEKHMLQAVENRYMAAVNGSGVFSGACTDGQTRYEAYLMQLRGKSAEFRAKQYSTFEKESMKYAARKQALIQKGHDCNAEEVIFSNYPIVASAMRPTFGYYTPIVKNPGIGSVINIMRPVWDKNSSISSPATLVGVGGFVQP a0a291feb8_gripa rpeGc3 protein protein Red alga Griffithsia pacifica MAFVSATSVAVSTRATSLTGRSVSSSNAPPARTPTRPSPCMAIEFQTYSGGLDRVSLEPYSITRYLFKPAPATVTDGDKDVAINAGLRQLFGNAYIMEEERAEFYNAESKFRCGEITAREFARAVALSNAYRSRFFNTVSQYRFFELNFKHFLGRAPLNQVEYSKHFKIFAEGGYEAEINSYFDDPEYDEVFGDDCMPFTRFRGTYAPINQFNRMCVLEGGFAMSDKQRPVQLMTSLAANVPPAAYRVVDGLPAIPNAEHPTRKFELPNASLERFRNEVEVAKARELQLRVELKEAYAKRDEYRSGFAGFRAMAADMDISMLPGPRFQGRVENYPTWDGKSAPWGKSGVDTLSGVEKRPAKEIAKKEFQLERIKQLVVDLERRVAVLEAEREQPALTPEPLMFELEGLPQIVKEEEVVEEVTTPGTGVSELSADFDSTTMVTPDIDPESEDSGIVAPKTVEVETSRLAKDVGDLPGKLMKELEEKTKAEGKEFGGPKDGRLSFPGDGSEMVIGG a0a291feb7_gripa rpeGc2 protein protein Red alga Griffithsia pacifica MVAFVAPTPSVASSATRFATGTTRLTNDANVPSVAATRRSAHAPPSMRVLDGLDTVTQDSYSFSRYVLGPFQPSVLNANSSEYEREIAIRAAYRHVFGNAYIMEEELAEVEVTASQYKLGNLTAKEFIRALAKSSAYKTRFFEGASQYRFVELNFMHLLGRAPDTQEEVATHMNIYHAKGFDAEIDSYIDSEEYDSVFGDYNVPFLRFRGAYTPCDSFNKQCALKGGWANSDKAMGGAALSGYNGSDGRQMCDRISAYVTSDTTDYESVAGNSPLLTTSPNWLAYPDPAIAPTPAFISPQEVREARARVEKLREAYNEEIAKTQARKNAMAPFRAMVEDMAPMLDRGVTFGDPMLVHPEAKLPENESALADLGGKSSDYKRFWSTMETNTVSRLERDLEEAKAELRVLEKGVDALTPMSTSVELRSASQDVNVGLDKEEEAIARPRIATKPRVKKVVDATEKRSLNLPGGIKINLPF a0a291feb6_gripa rpeG1 protein protein Red alga Griffithsia pacifica MSIPVLNYSLSTQNQRVYSFEYLPNEEQPKCYTTDNLPAAIEMDQIIWAAYRQIFSEHQLLSSTRQPFLESQLRFNQITVKDFIKGLILSDAFRYLNYDVNNNYRFVEMCIQRILGREIYNHREKLAFAVIIGSQGLEAFIDLLINSEEYEDNFGDNMIPYQRRRIIAQRSKGEIPFNLKTPRLGKDFLYKQGMPQLLWAGPVHRFRPQEQSPKAGDPALFLSMVQDLDGVL a0a291feb5_gripa rpeGr5 protein protein Red alga Griffithsia pacifica MAFVSATPVSQAVRPAPALGAQLAASPLRPEIAHASNSSTPRMGYGAYSYITDKTKGHVNQYYVDKFRIASDWTKGTPKTQADAVLGRTFKGAVLVPTEGIPQEFDPAIAPRDNTVDPDPRIAESEGEVYPWDINYFDPQFLPSAYSDVNDPETVDSSFADFRSSMWESRRESLTAQDFGAVARVQRIKNGLDEKYLMTLDGMLDARYARFQKIAEPAVLSPTGTPMTEIPGTPYLGSVGAMDFIAQEEESVAFWKSGPSTTPVNYKRPSGAQTPNLPYNTAAPVAAINEAQEAQKGQMQLSAGDDE a0a291feb4_gripa rpeGr6 protein protein Red alga Griffithsia pacifica MQAFIPSSSLSALTGAPVQKSSALTSLRTTRSATPCTTRMAAYPYTGSGYGGVGVPYANDKVGQLYKVTPTSNIVDTAASVSIFSTLVTLLAQTGLDYELKKSGPFTVFAPTNDAFTDLLNAHGFASFGPLLRPGNTDTLRDVLLYHVVRGTYDARDVVGKSVTVETMGGDEVTISCMKRKLVVGSSAVIRKDVSCSNGVIHVIKSVLKPPSYVRPDIRPQSQPMPESIVQDVYGKMLTPRQALGIDAAPESGALTSFYQ a0a291feb3_gripa rpeCz3 protein protein Red alga Griffithsia pacifica MYAFVNTTGVSATRASIPHSGLQTICGAPSRPAPATRSVSKAVIKMTGDYGITLDTLLYHPTPTISGVEDKDLICYSVWKQVFGNAYVMESERADAYVPESMFRAGQIPLREFVRGVALSATYRRRFFECCGPYRAVELNFRHLLGRAPVSQKEVSEHIKLIAAKGFEAEINSYIDSEEYEEAFGDDLVPYMRFKGTYTTCEEFNRMCTMYSAPGTTDKSLSIRARTQGIENPNHVLSLDGAGVPSKLVSIIAMGSHSSFVPVKRALPSRPDLEFGQSTKAPAQVNENANPVSRVEVCMGSYMYLTAEEAAQYNTDVMEQDQIASYAETEISEAETEIARLQAKIAELNLIK a0a291feb2_gripa rpeC1 protein protein Red alga Griffithsia pacifica MAFVPIVNLRPALSAPSTSSFSGVRLADAPTSTPAPVVARLGTVLGNSSLDKLGLDKFVDRFEVNEAGRPDGFSADYEVIIRACYMQIFANAYIMESERAEMAKAESEFRDGRFTVKEFCRALAKSYQYRKRFFDGRPLYGAIELCFKHILGRTPDGLEHYRAKSAVYDTKGYEAFIDAFFDDGEYDAFYDSYCVPFYRGHLTTSNLSMAAFTHMFQVVRGSSTSDKANPRTMTNQITLNQAGIQSIPLAVVAPGADGATFLAPDASAGSWQTGFSGATKARTSHGSRQEKGKMFRIEVANNTQYSAVGGGSGIKLQSRSGKFYKMRNMAPAKVSTFRRANNVYLVPFDELSATYIKIHKNGGSIASITPV a0a291feb1_gripa rpeGr4 protein protein Red alga Griffithsia pacifica MAAAFTAPVNLKGSSLTSNTLPAVCSRPAPLTLTPRAQADLPPPGIPSGQDPLDNAPLRHYVPRPVETYEDRGFATILPRTWEGETNTIGAGDIEPVTKEEVEESRKVPVDAASTGAFVEYARMMKEERAQALADQARRNSAPTSGRPTCGETEGTEFVSNARPILVDGVKVVEYWGVPNGPVPRLFGGPGE a0a291feb0_gripa rpeCz2 protein protein Red alga Griffithsia pacifica MASKLAFSSPLNFPVRTLLPTNTCVAANSSRTSVACPFTTSMAFSSIDRILTADPVAFPNGTESVIRATYIQVFGNAHVMDSEREELATAESNVCRTGNVREFVRAIVLSENYKSRFFYSVSQYRFIELMFKHILGRAPESRAEYAEAMAVYNTKGYEPLVSWFVDSLEYNENFGSWIVPYGIYQGCYSSNELFNRSVAMRLAPGCSDKGRSAMLQYCVLSGDSPNWLSISKALPAGTEKGTGLCIGGHWQSSQRNKQAPVKGKSKIPGGVIVTEE a0a291fea8_gripa apcA protein protein Red alga Griffithsia pacifica MSIVTKSIVNADAEARYLSPGELERIKSFVLSGQRRLRIAQTLTENREMIVKKGGQQLFQKRTDVVSPGGNAYGEEMTATCLRDLDYYLRLVTYGIVAGDVTPIEEIGLVGVKEMYNSLGTPISGVSEGVRCMKDVACSLLSGEDSAEAGFYFDYTLGAMQ a0a291fea5_gripa rpcA protein protein Red alga Griffithsia pacifica MKTPITEAIASADSQGRFLSNAELQSITGRYQRASASLEAAKQLTGNASRLITGAAQAVYTKFPFVTQMPGPTYASNAIGKAKCARDIGYYLRMTTYCLVVGATGPMDEYLVAGLEEINRSFELSPSWYIAALENIKNDHGLSGQAANEANTYLDYAINVLS a0a291fea4_gripa apcC protein protein Red alga Griffithsia pacifica MGYDMDGSGSGSLNPLRIKSSGVEKDRRAFKITYCLPSESRLFTYRELQNVYTTKLVPFSSWYAEQQRIQKMGGKIFKVELAAGGQMRSCGNS a0a291fea0_gripa apcB protein protein Red alga Griffithsia pacifica MQDAITAVINTADVQGKYLDDNSLDKLRGYFETGELRVRAAATIAANAATIIKESVAKALLYSDITRPGGNMYTTRRYAACIRDLDYYLRYATYGMLAGDPSILDERVLNGLKETYNSLGVPIGATVQAIQAMKEVTASLVGTNAGQEMAVYFDYICSGLS a0a291fe97_gripa rpcB protein protein Red alga Griffithsia pacifica MLDAFAKVVAQADARGEFLSNKQLNSLETMIGEGNKRLDIVNKINSNASTIVTNSARALFAEQPQLIQPGGNAYTSRRMAACLRDMEIVLRYVSYAMTAGDSSVLDDRCLNGLRETYQALGTPGTSVAVAIQKMKEASIALANDLNGVPLGDCSSLNSELAVYFDRAAAAVV a0a291fe95_gripa apcD protein protein Red alga Griffithsia pacifica VSIISEIILNADNELRYPSIGELQNLQDYFSTSESRIVISCILRDNEQQIIQAASNKIFQIHPDYIAPGGNAEGSRKRSLCLRDYGWYLRLVTYGLLAGDKQSIEKIGLIGVKEMYNSLGVPILGMIDAIDCLKNASINLLDEKQALIVSPYFDFIIQGMY a0a291fe93_gripa apcF protein protein Red alga Griffithsia pacifica MQDTIASIVNRYDCTGKYLDNVAISELESYFSTAIIRLKVIELINQSSSKIIQEAANRLYTEQPELLRPGGNSYTTRRYSACLRDIEYYLRYASYALIAGNTTILSERVLDGLKETYSSLNVPIGPTVRSIQLLEDIICNEFIILAQKQNISTNITFIQQPFNYMVKYLSEKNI apca-apcb-1 3943467 N/A 3943469 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943470 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943471 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943472 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 672.0 N/A 3943473 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943474 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943475 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943476 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943477 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943478 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 45.0 N/A 3943479 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943480 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943481 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943482 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 72.0 N/A 3943483 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943484 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943485 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943486 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943487 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943488 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3943489 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 N/A 3943490 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 3.0 physical association physical association false false false Fig 1, Extended Data Fig 2, Extended Data Table 1 apca-apcb-2 3943468 N/A 3943469 unspecified role unspecified role neutral component neutral component purified purified N/A 3943470 unspecified role unspecified role neutral component neutral component purified purified N/A 3943471 unspecified role unspecified role neutral component neutral component purified purified N/A 3943472 unspecified role unspecified role neutral component neutral component purified purified N/A 3943473 unspecified role unspecified role neutral component neutral component purified purified N/A 3943474 unspecified role unspecified role neutral component neutral component purified purified N/A 3943475 unspecified role unspecified role neutral component neutral component purified purified N/A 3943476 unspecified role unspecified role neutral component neutral component purified purified N/A 3943477 unspecified role unspecified role neutral component neutral component purified purified N/A 3943478 unspecified role unspecified role neutral component neutral component purified purified N/A 3943479 unspecified role unspecified role neutral component neutral component purified purified N/A 3943480 unspecified role unspecified role neutral component neutral component purified purified N/A 3943481 unspecified role unspecified role neutral component neutral component purified purified N/A 3943482 unspecified role unspecified role neutral component neutral component purified purified N/A 3943483 unspecified role unspecified role neutral component neutral component purified purified N/A 3943484 unspecified role unspecified role neutral component neutral component purified purified N/A 3943485 unspecified role unspecified role neutral component neutral component purified purified N/A 3943486 unspecified role unspecified role neutral component neutral component purified purified N/A 3943487 unspecified role unspecified role neutral component neutral component purified purified N/A 3943488 unspecified role unspecified role neutral component neutral component purified purified N/A 3943489 unspecified role unspecified role neutral component neutral component purified purified N/A 3943490 unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false Extended Data Fig 1