IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-2009-28 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK., Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK. Rare diseases - Interactions investigated in the context of Rare genetic disease The protein "Periostin" with GFP tag, a protein of 811 residues, was found to be of Rat origin (Ref. 34) and has only a ~94% match with any Uniprot Rat protein. Thus the Ref-Seq entry XP_006232398.1 has been used. eungkkim@yuhs.ac. The Journal of biological chemistry 2009 Only protein-protein interactions imex curation imex curation kim-2009-26 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK., Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK. Rare diseases - Interactions investigated in the context of Rare genetic disease Fibroblasts used were from an "hcf" cell line - derived from Human Corneal Fibroblasts. (see pmid:12714615) The protein "Periostin" with GFP tag, a protein of 811 residues, was found to be of Rat origin (Ref. 34) and has only a ~94% match with any Uniprot Rat protein. Thus the Ref-Seq entry XP_006232398.1 has been used. eungkkim@yuhs.ac. The Journal of biological chemistry 2009 Only protein-protein interactions imex curation imex curation kim-2009-27 human-hcf human corneal fibroblast cell line hcf human corneal fibroblast cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK., Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK. Rare diseases - Interactions investigated in the context of Rare genetic disease The protein "Periostin" with GFP tag, a protein of 811 residues, was found to be of Rat origin (Ref. 34) and has only a ~94% match with any Uniprot Rat protein. Thus the Ref-Seq entry XP_006232398.1 has been used. eungkkim@yuhs.ac. The Journal of biological chemistry 2009 Only protein-protein interactions imex curation imex curation kim-2009-29 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK., Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK. Rare diseases - Interactions investigated in the context of Rare genetic disease The protein "Periostin" with GFP tag, a protein of 811 residues, was found to be of Rat origin (Ref. 34) and has only a ~94% match with any Uniprot Rat protein. Thus the Ref-Seq entry XP_006232398.1 has been used. eungkkim@yuhs.ac. The Journal of biological chemistry 2009 Only protein-protein interactions imex curation imex curation kim-2009-30 human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK., Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK. Rare diseases - Interactions investigated in the context of Rare genetic disease Primary cultured corneal fibroblasts from a normal human patient and a GCD II (Type II granular corneal dystrophy) patient bearing homozygous R124H mutations in TGFBI. The protein "Periostin" with GFP tag, a protein of 811 residues, was found to be of Rat origin (Ref. 34) and has only a ~94% match with any Uniprot Rat protein. Thus the Ref-Seq entry XP_006232398.1 has been used. eungkkim@yuhs.ac. The Journal of biological chemistry 2009 Only protein-protein interactions imex curation imex curation kim-2009-31 human-hcf human corneal fibroblast cell line hcf human corneal fibroblast cell line confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK., Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK. Rare diseases - Interactions investigated in the context of Rare genetic disease The protein "Periostin" with GFP tag, a protein of 811 residues, was found to be of Rat origin (Ref. 34) and has only a ~94% match with any Uniprot Rat protein. Thus the Ref-Seq entry XP_006232398.1 has been used. eungkkim@yuhs.ac. The Journal of biological chemistry 2009 Only protein-protein interactions imex curation imex curation kim-2009-32 human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK., Kim BY., Olzmann JA., Choi SI., Ahn SY., Kim TI., Cho HS., Suh H., Kim EK. Rare diseases - Interactions investigated in the context of Rare genetic disease Primary cultured corneal fibroblasts from a normal human patient and a GCD II (Type II granular corneal dystrophy) patient bearing homozygous R124H mutations in TGFBI. The protein "Periostin" with GFP tag, a protein of 811 residues, was found to be of Rat origin (Ref. 34) and has only a ~94% match with any Uniprot Rat protein. Thus the Ref-Seq entry XP_006232398.1 has been used. eungkkim@yuhs.ac. The Journal of biological chemistry 2009 Only protein-protein interactions imex curation imex curation periostin_rat PERIOSTIN RAT protein protein rat Rattus norvegicus (Rat) Rat MVPLLPLSALLLLFLCDVDPANANSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAICGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSYTYFAPSNEAWDNLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNCARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDELSSFRAAAITSDLLESLGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNKKDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIFREIIQPAEKSLHEKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTNEEREILIGDKNALQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYVKGVNETLLVNELKSKESDIMTTNGVIHVVDKLLYPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYRPAMTKIHIEGEPDFRLIKEGETVTEVIHGEPVIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLQKFLQKEVSKVTKFIEGGDGHLFEDEAIKRLLQGDTPAKKIQANKRVQGSRRRSREGRSQ bgh3_human Transforming growth factor-beta-induced protein ig-h3 TGFBI BIGH3 Kerato-epithelin RGD-containing collagen-associated protein protein protein human Homo sapiens Human MALFVRLLALALALALGPAATLAGPAKSPYQLVLQHSRLRGRQHGPNVCAVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWASLPAEVLDSLVSNVNIELLNALRYHMVGRRVLTDELKHGMTLTSMYQNSNIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKDILATNGVIHYIDELLIPDSAKTLFELAAESDVSTAIDLFRQAGLGNHLSGSERLTLLAPLNSVFKDGTPPIDAHTRNLLRNHIIKDQLASKYLYHGQTLETLGGKKLRVFVYRNSLCIENSCIAAHDKRGRYGTLFTMDRVLTPPMGTVMDVLKGDNRFSMLVAAIQSAGLTETLNREGVYTVFAPTNEAFRALPPRERSRLLGDAKELANILKYHIGDEILVSGGIGALVRLKSLQGDKLEVSLKNNVVSVNKEPVAEPDIMATNGVVHVITNVLQPPANRPQERGDELADSALEIFKQASAFSRASQRSVRLAPVYQKLLERMKH postn_human Periostin POSTN OSF2 Osteoblast-specific factor 2 protein protein human Homo sapiens Human MIPFLPMFSLLLLLIVNPINANNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVYTTKIITKVVEPKIKVIEGSLQPIIKTEGPTLTKVKIEGEPEFRLIKEGETITEVIHGEPIIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLKKLLQEEVTKVTKFIEGGDGHLFEDEEIKRLLQGDTPVRKLQANKKVQGSRRRLREGRSQ Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion mint postn-tgfbi-2 3672719 N/A 3672726 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3672727 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed C-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Delta_N5_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n_terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B periostin_rat-tgfbi-1 3672719 N/A 3672726 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3672727 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Q15582:p.Arg124His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q15582:p.Arg124Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false Q15582:p.Arg124Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q15582:p.Arg555Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q15582:p.Arg555Gln mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false C-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 3D, 5A periostin_rat-tgfbi-2 3672719 N/A 3672726 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3672727 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3F tgfbi-periostin_rat-1 3672719 N/A 3672726 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3672727 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed C-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n_terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3H tgfbi-postn-1 3672720 N/A 3672728 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-hcf human corneal fibroblast cell line hcf human corneal fibroblast cell line N/A 3672727 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 2A Collagen type 6 was also pulled down but cannot be further identified. tgfbi-postn-2 3672720 N/A 3672728 unspecified role unspecified role bait bait purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3672727 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-hcf human corneal fibroblast cell line hcf human corneal fibroblast cell line physical association physical association false false false 2B Collagen type 6 was also pulled down but cannot be further identified. ertdfgcvb-1 3672721 N/A 3672728 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672727 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 2C postn-tgfbi-1 3672721 N/A 3672728 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672727 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 2D periostin_rat-tgfbi-3 3672722 N/A 3672726 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3672727 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed C-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false 4A postn-tgfbi-3 3672723 N/A 3672728 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672727 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level Q15582:p.Arg124His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 5C periostin_rat-tgfbi-4 3672724 N/A 3672728 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672727 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false 4B postn-tgfbi-4 3672725 N/A 3672728 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3672727 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level Q15582:p.Arg124His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Figure 6D