IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ brown-2005-8 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Brown JH., Zhou Z., Reshetnikova L., Robinson H., Yammani RD., Tobacman LS., Cohen C. ccohen@brandeis.edu Proceedings of the National Academy of Sciences of the United States of America 2005 Only protein-protein interactions imex curation imex curation tpm1_rat Tropomyosin alpha-1 chain Tpm1 Alpha-tm Tpma Tropomyosin-1 Alpha-tropomyosin protein protein rat Rattus norvegicus (Rat) Rat MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI tpm1-1 3710184 N/A 3710185 unspecified role unspecified role neutral component neutral component purified purified tropomyosin's mid-region tropomyosin's mid-region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminal GC4 tag C-terminal GC4 tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminal GC4-tag from yeas GC4 (UniProt P03069): 255-280 ecoli Escherichia coli (strain K12) difference in PDB and UniProt sequence: Met at position 88. Is that an N-terminal tag? How to annotate it? Stoichiometry: 1.0 N/A 3710185 unspecified role unspecified role neutral component neutral component purified purified tropomyosin's mid-region tropomyosin's mid-region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminal GC4-tag C-terminal GC4-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminal GC4-tag from yeas GC4 (UniProt P03069): 255-280 ecoli Escherichia coli (strain K12) difference in PDB and UniProt sequence: Met at position 88. Is that an N-terminal tag? How to annotate it? Stoichiometry: 1.0 3710191 3710188 direct interaction direct interaction false false false 1/a Rwork=0.256 Rfree=0.296 2.3Å