DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ cui-2019-1 in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp Cui Y., Parashar S., Zahoor M., Needham PG., Mari M., Zhu M., Chen S., Ho HC., Reggiori F., Farhan H., Brodsky JL., Ferro-Novick S. sfnovick@ucsd.edu Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation cui-2019-2 yeast Saccharomyces cerevisiae Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging experimental particp experimental participant identification experimental particp Cui Y., Parashar S., Zahoor M., Needham PG., Mari M., Zhu M., Chen S., Ho HC., Reggiori F., Farhan H., Brodsky JL., Ferro-Novick S. sfnovick@ucsd.edu Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation atg40_yeast Autophagy-related protein 40 ATG40 O3536 YOR152C protein protein yeast Saccharomyces cerevisiae Baker's yeast MFNLILWPLFLLTSVAIPLQLTLEVVYLTSSVDFSKASAAKTATSLGQSPVVITIYKSLLKYWSLYEFIHFIYLYTPIDAFLNFLPFTSLLMSFGSICLTRELVYDFIAFMESQNKLTGFLNKITEPNFNSYLLFSSIYNIWFADDTNDKFLFGKLTQILISVTKRYEFPRTFYLAKVSDFLQNLILTRLRPFVTEQPQGDKNRYQNGDRESTKNGAAYQKSSQQSSSFEQNFTSTEFPNDYDFMEDILDETTELD FD4951C817E2AD0F sfb3_yeast SED5-binding protein 3 SFB3 SEC24-related protein 3 Lethal with SEC13 protein 1 LST1 YHR098C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQQNILAASVSALSLDESTVHTGGASSKKSRRPHRAYHNFSSGTVPTLGNSPYTTPQLNQQDGFQQPQAFTPKQFGGFNNGSGSVMSTPVMVSQERFGASEASSPYGQSMLDMTAPQPTSHIVPTQRFEDQAQYLQRSFETCRDSVPPLPTTQFYCVDQGSCDPHLMSLSMYNIPESEHLRAATKLPLGLTIQPFSTLTPNDAEVPTIPLPMDGTPLRCRRCRAYANPKFQFTYDSSVICNICRVKMQVPGEHFAPMGPNGQRSDLNEKSELLHGTVDFLVPSIYNAIQEKELLPLHYVFLIDVSLLANENGSSLAMVEGVRSCIEYISDFQPNCEVAIIVYDNKLRFFNLRPDLDNAQEYIVSELDDVFLPFYNGLFVKPGNSMKIINDTLIKISGYISTDKYSHVPQVCYGSALQAAKLALDTVTGGQGGKIICSLNSLPTIGNGNLSLKRDNAHIAHVKCDNGFYKKLASDFLKSYISLDLYVTNAGFIDMATVGHPVEMTSGILKYYPHFQQETDAFTLVNDMVTNVSNIVGYQALLKVRCSTGLSVEQYYCDSSDNTDHDPIIPVLTRDTTLDVLLKYDSKIKTGTDVHFQTALLYTDIDGVRKVRSINTSGAVSNNIREIFKFINQNPVMRIMIKDVIKTLGDCDFVKIRRLIDDKMVEILTQYRGLVSSNSSTQLILPDSIKTLPAYMLAFEKSELMKPNAQSTRGNERIYDLLKYDSLNSAQLCYKLYPQIVPFHVLLEETDLTFYDANDKLLQINSSSINNLSVRASHSNFINGGCYLIFQGDTIYLWFNENTNRMLLQDLLSVDESLPVSQISLFSGTLPETGTSINQKASNVIKNWQQVVNKSSLPLVLLRPNVDQYYSNVMSQLLCEDKTVNRIESYDNYLVIMHKKIQEKLQKDDFIKVSTAATHENIHQKFVQF 6A028AD6B1A2E031 atg40-1 3581324 N/A 3581326 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeast Saccharomyces cerevisiae Baker's yeast N/A 3581327 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 3A sfb3-atg40-1 3581325 N/A 3581326 tag visualisation tag visualisation unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level GFP Tag GFP Tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeast Saccharomyces cerevisiae Baker's yeast N/A 3581327 tag visualisation tag visualisation unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level mCherry Tag mCherry Tag mcherry mcherry fluorescent protein tag mcherry c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeast Saccharomyces cerevisiae Baker's yeast colocalization colocalization false false false Fig 2A Rapamycin (CHEBI:9168)