UniProt UniProt http://www.uniprot.org oroz-2018-1 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-2 in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Source of AHSA1 taken from work by the same author (PPR:PPR70419) Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-4 in vitro In vitro fluorescence fluorescence technology fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. For affinity calculations IANBD amide (N,N′-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine fluorescent dye was measured in the presence of increasing concentrations of binding protein. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-6 in vitro In vitro crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Cross linker:BS3 (bis(sulfosuccinimidyl) suberate. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-5 in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-8 in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. For affinity calculations IANBD amide (N,N′-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine fluorescent dye was measured in the presence of increasing concentrations of binding protein. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation oroz-2018-9 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Oroz J., Chang BJ., Wysoczanski P., Lee CT., Pérez-Lara Á., Chakraborty P., Hofele RV., Baker JD., Blair LJ., Biernat J., Urlaub H., Mandelkow E., Dickey CA., Zweckstetter M. Markus.Zweckstetter@dzne.de Nature communications 2018 Only protein-protein interactions imex curation imex curation hs90b_human Heat shock protein HSP 90-beta HSP90AB1 HSP90B HSPC2 HSPCB Heat shock 84 kDa protein protein human Homo sapiens Human MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) fkbp5_human Peptidyl-prolyl cis-trans isomerase FKBP5 FKBP5 AIG6 FKBP51 FK506-binding protein 5 51 kDa FK506-binding protein Rotamase 54 kDa progesterone receptor-associated immunophilin FKBP54 FF1 antigen HSP90-binding immunophilin Androgen-regulated protein 6 protein protein human Homo sapiens Human MTTDEGAKNNEESPTATVAEQGEDITSKKDRGVLKIVKRVGNGEETPMIGDKVYVHYKGKLSNGKKFDSSHDRNEPFVFSLGKGQVIKAWDIGVATMKKGEICHLLCKPEYAYGSAGSLPKIPSNATLFFEIELLDFKGEDLFEDGGIIRRTKRKGEGYSNPNEGATVEIHLEGRCGGRMFDCRDVAFTVGEGEDHDIPIGIDKALEKMQREEQCILYLGPRYGFGEAGKPKFGIEPNAELIYEVTLKSFEKAKESWEMDTKEKLEQAAIVKEKGTVYFKGGKYMQAVIQYGKIVSWLEMEYGLSEKESKASESFLLAAFLNLAMCYLKLREYTKAVECCDKALGLDSANEKGLYRRGEAQLLMNEFESAKGDFEKVLEVNPQNKAARLQISMCQKKAKEHNERDRRIYANMFKKFAEQDAKEEANKAMGKKTSEGVTNEKGTDSQAMEEEKPEGHV p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL amp-pnp AMP-PNP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ahsa1_human Activator of 90 kDa heat shock protein ATPase homolog 1 HSPC322 p38 AHSA1 C14orf3 protein protein human Homo sapiens Human MAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF hsp90ab1-4 3559684 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1B, Supp. Fig. 1B adp-hsp90ab1-1 3559684 N/A 3559694 unspecified role unspecified role neutral component neutral component N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1B, Supp. Fig. 1B fkbp5-hsp90ab1-3 3559684 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1B, Supp. Fig. 1B fkbp5-hsp90ab1-4 3559684 N/A 3559694 unspecified role unspecified role unspecified role unspecified role N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false Fig. 1B, Supp. Fig. 1B fkbp5-hsp90ab1-5 3559684 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false Fig. 1B, Supp. Fig. 1B adp-hsp90ab1-2 3559684 N/A 3559697 unspecified role unspecified role neutral component neutral component N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 1B hsp90ab1-mapt-4 3559684 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind range range range range false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 1B adp-fkbp5-1 3559684 N/A 3559694 unspecified role unspecified role neutral component neutral component N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 1B amp_pnp-fkbp5-1 3559684 N/A 3559697 unspecified role unspecified role neutral component neutral component N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 1B amp_pnp-fkbp5-2 3559684 N/A 3559697 unspecified role unspecified role unspecified role unspecified role N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false Supp. Fig. 1B fkbp5-hsp90ab1-6 3559685 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 1D, Supp Fig.1C-D, 2C-E, 3A fkbp5-hsp90ab1-9 3559685 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 4D, Supp. Fig 6C hsp90ab1-mapt-6 3559685 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 4D, 4H Phosphorylation of MAPT by the microtubule affinity-regulating kinase and insertion of glutamic acid at specific phosphorylation epitopes abolished/weakened MAPTs interaction with HSP90AB1 and FKBP5. fkbp5-mapt-1 3559685 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 4D,4H, Supp Fig. 6B Phosphorylation of MAPT by the microtubule affinity-regulating kinase and insertion of glutamic acid at specific phosphorylation epitopes abolished/weakened MAPTs interaction with HSP90AB1 and FKBP5. fkbp5-hsp90ab1-12 3559685 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified mstl tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Supp Table 5, Supp. Fig. 7A hsp90ab1-mapt-8 3559685 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified mstl tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Supp Table 5, SUpp. Fig. 7A fkbp5-hsp90ab1-13 3559685 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified region 13c label 13c label C13 13C C13 13C undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2B-D atp-hsp90ab1-1 3559686 N/A 3559698 enzyme target enzyme target substrate substrate neutral component neutral component N/A 3559693 enzyme enzyme neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) atpase reaction atpase reaction false false false Fig. 1C ahsa1-atp-1 3559686 N/A 3559699 enzyme regulator enzyme regulator unspecified role unspecified role purified purified n-terminal t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3559698 enzyme target enzyme target substrate substrate neutral component neutral component N/A 3559693 enzyme enzyme neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) atpase reaction atpase reaction false false false Fig. 1C AHSA1 enhances HSP90AB1 ATPase activity atp-fkbp5-1 3559686 N/A 3559695 enzyme regulator enzyme regulator neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3559693 enzyme enzyme neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 3559698 enzyme target enzyme target substrate substrate neutral component neutral component atpase reaction atpase reaction false false false Fig. 1C Full length FKBP5, but not the intrinsically disordered MAPT decreased the rate of HSP90AB1 ATP hydrolysis fkbp5-hsp90ab1-7 3559687 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3559695 ancillary ancillary neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P08238:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false cys-363 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 3559687 Fig. 4A HSP90AB1 is pre-bound to MAPT. hsp90ab1-mapt-5 3559687 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P08238:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false cys-363 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 3559687 Fig. 4A fkbp5-fkbp5-1 3559687 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified tpr repeat region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P08238:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain false cys-363 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3559687 Fig. 2H fkbp5-hsp90ab1-10 3559688 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified lys-60 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-8 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-60 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-38 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-415 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-230 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-400 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lys-354 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-402 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-406 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-491 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-551 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-557 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-577 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-623 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-685 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 3559869 3559851 3559870 3559850 3559871 3559852 3559872 3559853 3559873 3559854 3559874 3559855 3559875 3559856 3559876 3559857 3559877 3559865 3559878 3559858 3559879 3559859 3559880 3559860 3559881 3559861 3559882 3559862 3559883 3559863 3559884 3559864 direct interaction direct interaction false false false Supp. Fig. 3B, Supplementary Table 2 hsp90ab1-mapt-7 3559688 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-370 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-395 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-353 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-257 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-298 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-267 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-267 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-294 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-370 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-2 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-163 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-180 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-44 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-240 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-148 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-234 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-291 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-386 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-163 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-399 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-481 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-505 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-505 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-552 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-573 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-623 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-624 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-649 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-649 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false lys-64 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-180 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-186 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-199 125i radiolabel 125i radiolabel I125 125I 125I I125 certain certain sequence position certain certain certain sequence position certain false lys-204 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-219 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-284 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-286 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-347 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-347 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-354 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-354 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-435 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-481 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 3559919 3559888 3559920 3559890 3559921 3559916 3559922 3559892 3559923 3559893 3559924 3559894 3559925 3559889 3559926 3559895 3559927 3559896 3559928 3559887 3559929 3559897 3559930 3559898 3559933 3559899 3559934 3559900 3559935 3559901 3559936 3559902 3559937 3559903 3559938 3559904 3559939 3559905 3559940 3559906 3559941 3559907 3559942 3559908 3559943 3559909 3559944 3559910 3559945 3559911 3559946 3559912 3559947 3559913 3559948 3559914 3559949 3559915 3559950 3559891 3559951 3559917 direct interaction direct interaction false false false Supp. Fig. 3b, Supp. Table 3 fkbp5-hsp90ab1-11 3559688 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified lys-375 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-281 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-257 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-395 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-225 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-190 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-369 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-370 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-317 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-140 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-395 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-383 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified lys-352 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-385 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-60 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-272 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-274 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-283 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-283 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-283 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-28 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-58 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-88 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-153 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-272 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-155 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-155 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-280 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 3559975 3559955 3559976 3559956 3559977 3559973 3559978 3559957 3559979 3559958 3559980 3559960 3559981 3559961 3559982 3559962 3559983 3559963 3559984 3559964 3559985 3559954 3559986 3559972 3559987 3559965 3559988 3559966 3559989 3559967 3559990 3559968 3559991 3559969 3559992 3559970 3559993 3559959 3559994 3559971 physical association physical association false false false Fig. 3B, Supplementary Table 4 fkbp5-hsp90ab1-8 3559689 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false Fig. 4C, Supplementary Figure 8. hsp90ab1-9 3559690 N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Fig. 1A fkbp5-hsp90ab1-15 3559691 N/A 3559696 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3559691 Supp Fig. 6A HSP90AB1 is pre-bound to MAPT. fkbp5-hsp90ab1-16 3559692 N/A 3559695 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3559693 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3559692 Supp. Fig 5 B/C