IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tena_campos-2015-2 human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Tena-Campos M., Ramon E., Borroto-Escuela DO., Fuxe K., Garriga P. pere.garriga@upc.edu Biochimica et biophysica acta 2015 Only protein-protein interactions imex curation imex curation tena_campos-2015-1 human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Tena-Campos M., Ramon E., Borroto-Escuela DO., Fuxe K., Garriga P. pere.garriga@upc.edu Biochimica et biophysica acta 2015 Only protein-protein interactions imex curation imex curation tena_campos-2015-3 human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tena-Campos M., Ramon E., Borroto-Escuela DO., Fuxe K., Garriga P. pere.garriga@upc.edu Biochimica et biophysica acta 2015 Only protein-protein interactions imex curation imex curation tena_campos-2015-4 human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tena-Campos M., Ramon E., Borroto-Escuela DO., Fuxe K., Garriga P. pere.garriga@upc.edu Biochimica et biophysica acta 2015 Only protein-protein interactions imex curation imex curation gpr39_human G-protein coupled receptor 39 GPR39 protein protein human Homo sapiens Human MASPSLPGSDCSQIIDHSHVPEFEVATWIKITLILVYLIIFVMGLLGNSATIRVTQVLQKKGYLQKEVTDHMVSLACSDILVFLIGMPMEFYSIIWNPLTTSSYTLSCKLHTFLFEACSYATLLHVLTLSFERYIAICHPFRYKAVSGPCQVKLLIGFVWVTSALVALPLLFAMGTEYPLVNVPSHRGLTCNRSSTRHHEQPETSNMSICTNLSSRWTVFQSSIFGAFVVYLVVLLSVAFMCWNMMQVLMKSQKGSLAGGTRPPQLRKSESEESRTARRQTIIFLRLIVVTLAVCWMPNQIRRIMAAAKPKHDWTRSYFRAYMILLPFSETFFYLSSVINPLLYTVSSQQFRRVFVQVLCCRLSLQHANHEKRLRVHAHSTTDSARFVQRPLLFASRRQSSARRTEKIFLSTFQSEAEPQSKSQSLSLESLEPNSGAKPANSAAENGFQEHEV mint homomint 5ht1a_human 5-hydroxytryptamine receptor 1A HTR1A ADRB2RL1 ADRBRL1 G-21 Serotonin receptor 1A protein protein human Homo sapiens Human MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ galr1_human Galanin receptor type 1 GALR1 GALNR GALNR1 protein protein human Homo sapiens Human MELAVGNLSEGNASWPEPPAPEPGPLFGIGVENFVTLVVFGLIFALGVLGNSLVITVLARSKPGKPRSTTNLFILNLSIADLAYLLFCIPFQATVYALPTWVLGAFICKFIHYFFTVSMLVSIFTLAAMSVDRYVAIVHSRRSSSLRVSRNALLGVGCIWALSIAMASPVAYHQGLFHPRASNQTFCWEQWPDPRHKKAYVVCTFVFGYLLPLLLICFCYAKVLNHLHKKLKNMSKKSEASKKKTAQTVLVVVVVFGISWLPHHIIHLWAEFGVFPLTPASFLFRITAHCLAYSNSSVNPIIYAFLSENFRKAYKQVFKCHIRKDSHLSDTKESKSRIDTPPSTNCTHV gpr39-htr1a-2 3575639 N/A 3575643 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3575644 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 1G, 2B Both receptors share a similar distributional pattern and significantly co-localized in the membrane cell surface and reticular compartments. htr1a-gpr39-3 3575639 N/A 3575644 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3575645 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 2B 5-HT1A maintained its capacity to interact with GalR1in the presence of GPR39. gpr39-htr1a-1 3575640 N/A 3575643 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3575644 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus rho tag rho tag 1D4 tag rho1D4 tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Figure 1 A-F Both receptors share a similar distributional pattern and significantly co-localized in the membrane cell surface and reticular compartments. gpr39-htr1a-3 3575641 N/A 3575643 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3575644 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminus rho tag rho tag 1D4 tag rho1D4 tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 2A-c htr1a-gpr39-1 3575642 N/A 3575643 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c-terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3575645 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3575644 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c-terminus rho tag rho tag 1D4 tag rho1D4 tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false Figure 2A-d