IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ han-2011-4 in vitro In vitro uv-vis ultraviolet-visible spectroscopy uv-vis predetermined predetermined participant predetermined Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. donghak@konkuk.ac.kr Biological & pharmaceutical bulletin 2011 Only protein-protein interactions imex curation imex curation han-2011-5 in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. The reaction was studied using dilauroylphosphatidylcholine (DLPC) reconstituted systems donghak@konkuk.ac.kr Biological & pharmaceutical bulletin 2011 Only protein-protein interactions imex curation imex curation han-2011-6 in vitro In vitro oxidoreduct assay oxidoreductase assay oxidoreduct assay predetermined predetermined participant predetermined Han S., Choi S., Chun YJ., Yun CH., Lee CH., Shin HJ., Na HS., Chung MW., Kim D. The reaction was studied using dilauroylphosphatidylcholine (DLPC) reconstituted systems NADPH Oxidation Assay (Table 3) was not captured as the participants were not purified donghak@konkuk.ac.kr Biological & pharmaceutical bulletin 2011 Only protein-protein interactions imex curation imex curation cp2a6_human Cytochrome P450 2A6 1,4-cineole 2-exo-monooxygenase CYPIIA6 Coumarin 7-hydroxylase Cytochrome P450 IIA3 Cytochrome P450(I) CYP2A6 CYP2A3 protein protein human Homo sapiens Human MLASGMLLVALLVCLTVMVLMSVWQQRKSKGKLPPGPTPLPFIGNYLQLNTEQMYNSLMKISERYGPVFTIHLGPRRVVVLCGHDAVREALVDQAEEFSGRGEQATFDWVFKGYGVVFSNGERAKQLRRFSIATLRDFGVGKRGIEERIQEEAGFLIDALRGTGGANIDPTFFLSRTVSNVISSIVFGDRFDYKDKEFLSLLRMMLGIFQFTSTSTGQLYEMFSSVMKHLPGPQQQAFQLLQGLEDFIAKKVEHNQRTLDPNSPRDFIDSFLIRMQEEEKNPNTEFYLKNLVMTTLNLFIGGTETVSTTLRYGFLLLMKHPEVEAKVHEEIDRVIGKNRQPKFEDRAKMPYMEAVIHEIQRFGDVIPMSLARRVKKDTKFRDFFLPKGTEVYPMLGSVLRDPSFFSNPQDFNPQHFLNEKGQFKKSDAFVPFSIGKRNCFGEGLARMELFLFFTTVMQNFRLKSSQSPKDIDVSPKHVGFATIPRNYTMSFLPR coumarin coumarin 1,2-Benzopyrone 2-Propenoic acid, 3-(2-hydroxyphenyl)-, d-lactone 2-Propenoic acid, 3-(2-hydroxyphenyl)-, delta-lactone 2H-1-Benzopyran-2-one 2H-benzo[b]pyran-2-one 5,6-Benzo-2-pyrone Benzo-a-pyrone Benzo-alpha-pyrone cis-o-Coumarinic acid lactone Coumarine Coumarinic anhydride Cumarin o-hydroxycinnamic acid delta-lactone o-Hydroxycinnamic acid lactone Rattex Tonka bean camphor 2H-chromen-2-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) nicotine nicotine (+-)-nicotine (+-)-3-(1-Methyl-2-pyrrolidinyl)pyridine (R,S)-nicotine (RS)-nicotine Nicotin Nikotin 3-(1-methylpyrrolidin-2-yl)pyridine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) coumarin-cyp2a6-1 3577732 N/A 3577735 unspecified role unspecified role neutral component neutral component purified purified P11509:p.Ile471Thr CYP2A6*7 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P11509:p.Arg485Leu CYP2A6*8 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P11509:p.Tyr392Phe CYP2A6*18 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P11509:p.Asn438Tyr CYP2A6*35 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P11509:p.[Tyr392Phe;Ile471Thr] CYP2A6*19 mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3577736 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3577732 Table 1, Figure 2 coumarin-cyp2a6-2 3577733 N/A 3577736 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3577735 enzyme enzyme neutral component neutral component purified purified P11509:p.Ile471Thr CYP2A6*7 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P11509:p.Tyr392Phe CYP2A6*18 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P11509:p.Arg485Leu CYP2A6*8 mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P11509:p.[Tyr392Phe;Ile471Thr] CYP2A6*19 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P11509:p.Asn438Tyr mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) hydroxylation hydroxylation reaction hydroxylation false false false 3577733 Table 2 cyp2a6-nicotine-1 3577734 N/A 3577737 enzyme target enzyme target substrate substrate neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3577735 enzyme enzyme neutral component neutral component purified purified P11509:p.Ile471Thr CYP2A6*7 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P11509:p.Arg485Leu CYP2A6*8 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P11509:p.Tyr392Phe CYP2A6*18 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P11509:p.[Tyr392Phe;Ile471Thr] CYP2A6*19 mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P11509:p.Asn438Tyr CYP2A6*35 mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) redox reaction oxidoreductase activity electron transfer reaction redox reaction false false false 3577734 Table 2, Figure 3