IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ li-2010-51 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Li J., Hand LE., Meng QJ., Loudon AS., Bechtold DA. Table 1 preys, Gametogentin, Syndecan binding protein 8 and Spermatogenesis were not found to have UniProtKB id, so not able to capture as interaction in Y2H assay david.bechtold@manchester.ac.uk,andrew.loudon@manchester.ac.uk mouse testis cDNA library PloS one 2010 Only protein-protein interactions imex curation imex curation li-2010-52 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li J., Hand LE., Meng QJ., Loudon AS., Bechtold DA. david.bechtold@manchester.ac.uk,andrew.loudon@manchester.ac.uk PloS one 2010 Only protein-protein interactions imex curation imex curation li-2010-53 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Li J., Hand LE., Meng QJ., Loudon AS., Bechtold DA. david.bechtold@manchester.ac.uk,andrew.loudon@manchester.ac.uk PloS one 2010 Only protein-protein interactions imex curation imex curation kat5_mouse Histone acetyltransferase KAT5 Kat5 Htatip Tip60 Lysine acetyltransferase 5 Histone acetyltransferase HTATIP 60 kDa Tat-interactive protein protein protein mouse Mus musculus Mouse MAEVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGSARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW mtr1l_mouse Melatonin-related receptor G protein-coupled receptor 50 H9 Gpr50 protein protein mouse Mus musculus Mouse MATVPKSNMGPTKAVPTPFGCIGCKLPKPDYPPALIIFMFCAMVITVVVDLIGNSMVILAVTKNKKLRNSGNIFVASLSVADMLVAIYPYPLMLYAMSVGGWDLSQLQCQMVGLVTGLSVVGSIFNITAIAINRYCYICHSLQYKRIFSLRNTCIYLVVTWVMTVLAVLPNMYIGTIEYDPRTYTCIFNYVNNPAFTVTIVCIHFVLPLIIVGYCYTKIWIKVLAARDPAGQNPDNQFAEVRNFLTMFVIFLLFAVCWCPVNVLTVLVAVIPKEMAGKIPNWLYLAAYCIAYFNSCLNAIIYGILNESFRREYWTIFHAMRHPILFISHLISDIRETWETRALTRARVRARDQVREQERARACVAVEGTPRNVRNVLLPGDASAPHSDRASVRPKPQTRSTSVYRKPASIHHKSISGHPKSASVYPKPASSVHCKPASVHFKPASVHFKGDSVYFKGDTVHYRAASKLVTSHRISAGPSTSHPTSMAGYIKSGTSHPATTTVDYLEPATTSHSVLTAVDLPEVSASHCLEMTSTGHLRADISASVLPSVPFELAATPPDTTAIPIASGDYRKVVLIDDDSDDSDCSDEMAV ctxn1_mouse Cortexin-1 Ctxn1 Ctxn protein protein mouse Mus musculus Mouse MSSAWTLSPEPLPPSTGPPVGAGLDVEQRTVFAFVLCLLVVLVLLMVRCVRILLDPYSRMPASSWTDHKEALERGQFDYALV snx5_mouse Sorting nexin-5 Snx5 protein protein mouse Mus musculus Mouse MAAVPELLEQQEEDRSKLRSVSVDLNVDPSLQIDIPDALSERDKVKFTVHTKTTLSTFQSPEFSVTRQHEDFVWLHDTLTETTDYAGLIIPPAPTKPDFDGPREKMQKLGEGEGSMTKEEFAKMKQELEAEYLAVFKKTVSTHEVFLQRLSSHPVLSKDRNFHVFLEYDQDLSVRRKNTKEMFGGFFKSVVKSADEVLFSGVKEVDDFFEQEKNFLINYYNRIKDSCAKADKMTRSHKNVADDYIHTAACLHSLALEEPTVIKKYLLKVAELFEKLRKVEGRVSSDEDLKLTELLRYYMLNIEAAKDLLYRRTKALIDYENSNKALDKARLKSKDVKLAETHQQECCQKFEQLSESAKEELINFKRKRVAAFRKNLIEMSELEIKHARNNVSLLQSCIDLFKNN snx6_mouse Sorting nexin-6 Snx6 protein protein mouse Mus musculus Mouse MMEGLDDGPDFLSEEDRGLKAINVDLQSDAALQVDISDALSERDRVKFTVHTKSSLPNFKQNEFSVVRQHEEFIWLHDSFVENEDYAGYIIPPAPPRPDFDASREKLQKLGEGEGSMTKEEFTKMKQELEAEYLAIFKKTVAMHEVFLCRVAAHPILRKDLNFHVFLEYNQDLSVRGKNKKEKLEDFFKNMVKSADGVIVSGVKDVDDFFEHERTFLLEYHNRVKDASAKSDRMTRSHKSAADDYNRIGSSLYALGTQDSTDICKFFLKVSELFDKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKALDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELKHAKGNLQLLQNCLAVLNGDT mint May be involved in several stages of intracellular trafficking kif9_mouse Kinesin-like protein KIF9 Kif9 protein protein mouse Mus musculus Mouse MGTRKKVQAFVRVRPTDDFAHEMIKYGEDNKSIDIHLKKDTRRGVVNNQQTDWSFKLDGVLHNASQDLVYETVAKDAVSQALDGYNGTIMCYGQTGAGKTYTMTGATENYKHRGILPRALQQVFRMIEERPTHAITVRVSYLEIYNENLFDLLSTLPYVGPSVTPMTIVENPQGIFIKGLSVHLTSQEEDAFSLLFEGETNRIIASHTMNKNSSRSHCIFTIYMEAHSRTLSDEKYITSKINLVDLAGSERLSKTGSEGRVLKEATYINKSLSFLEQAIIALGDQNRDHVPFRQSKLTHALKDSLGGNCNMVLVTNIYGEAAQLDETLSSLRFASRMKLVTTEPAINEKYDAERMVKNLEKELALLKQELAIHDSLSNRTLVNYDPMDEIQIAEINSQVRRYLEGTLDEIDIINLRQIQEVFNQFRVVLSQQEQEVESALRRKYTLIDKNDFAAISAVQKVGLMDIEGNLVGEPDGQSFGLGVAPFSVKPGKKPKTKKTPKDQFSSSARKEGASSPVSGKDFDVASISKTQLIPSSKDGDLKDMLARERETSSIEPLISDSPKEELRAPRPSTPPSRTVAFEEFKNERGSEINRIFKENKSILNERKKRASETTQRINAIKQEIDETKDALNFQKSLREKQGEYENKGLMIIDEEEFLLILKLKDLKKQYRNEYQELRDLRAEIQYCQRLVDQCRHRLLMEFDIWYNESFMIPEDVQVALKLGSSIRPGMVPISRIVCLGEDDQDRFSHLQQTVLPEGLDSITFYNAKVKTDQKHNYMKTMVGLQQSHRK aka10_mouse A-kinase anchor protein 10, mitochondrial Protein kinase A-anchoring protein 10 Dual specificity A kinase-anchoring protein 2 Akap10 protein protein mouse Mus musculus Mouse MRGAGPSPRHSPRALRPDPGPAMSFFRRKVKGKEQEKTLDVKSTKASVAVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLRDTVVLPYFLQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKRHETPASSVTEALDRRLGDSSSAPLLVTQSEGTDLSSRTQNPQNHLLLSQEGHSARSLHREVARTGSHQIPTDSQDSSSRLAVGSRNSCSSPLRELSEKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIAKICGEDGQVDPNCFVLAQAVVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLAAHGSVCLPEESHSGGSDGSTAQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMSFGRVSDLGQFIRESEPEPDVKKSKGFMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDVMQQAHHDQPLEKSTKL pex2_mouse Peroxisome biogenesis factor 2 Pex2 Paf1 Pxmp3 Pmp35 Peroxin-2 Peroxisomal membrane protein 3 Peroxisome assembly factor 1 protein protein mouse Mus musculus Mouse MAAREESTQSANRVLRISQLDALELNKALEQLVWSQFTQCFHGFKPGLLARFEPEVKAFLWLFLWRFTIYSKNATVGQSVLNIQHKNDSSPNPVYQPPSKNQKLLYAVCTIGGRWLEERCYDLFRNRHLASFGKAKQCMNFVVGLLKLGELMNFLIFLQKGKFATLTERLLGIHSVFCKPQNMREVGFEYMNRELLWHGFAEFLIFLLPLINIQKLKAKLSSWCTLCTGAAGHDSTLGSSGKECALCGEWPTMPHTIGCEHVFCYYCVKSSFLFDIYFTCPKCGTEVHSVQPLKAGIQMSEVNAL q8vdv7_mouse Tbc1d19 protein protein mouse Mus musculus Mouse MLQEESDLSLIIAQIVQKLKGSNLYAQLERQAWSCLQRPEIRLESLKEDIKEFFKISGWEKKLQNAVYSELNVFPLPSHPSAPPEHLKEPLVYMRKAQGSWEKRILKSLNSMCTELSIPLARKRPAMEQKELLNKWNEMGTDEPDLSLFRPVYAPKDFLEVLINLRNPNYENGDSLSFRTHLGLIQVPLKVKDIPELKECFVELGLNTGQLGIDDSTQVPPELFENEHVRIGQKVLAQQDSAAAQQYIRQGSPTALRAELWALILNISSQPEDILYYEQLKTNVIQHDLLVDSLIYKDVKLTASNDDYYFVFEDYLYQVLLCFSRDTSVLGHFAYNSASPPKSYIRGKLGLEEYAVFYPPNGVIPFHGFSMYVAPLCFLYHEPYKLYQIFREMYVRFFFRLHSISSHPSGIVSLCLLFETLLQTYLPQLFYHLREIGAQPLRISFKWMVRAFSGYLATDQLLLLWDRILGYNSLEILAVLAAAVFAFRAVNLMEVTSLAAAEAVLADLSTLKVMPLLQIFLFATVT gpr50-kat5-1 3547950 N/A 3547953 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-1 3547950 N/A 3547955 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-snx5-1 3547950 N/A 3547956 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-snx6-1 3547950 N/A 3547957 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-kif9-1 3547950 N/A 3547958 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-akap10-1 3547950 N/A 3547959 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-pex2-1 3547950 N/A 3547960 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-tbc1d19-1 3547950 N/A 3547961 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table 1 gpr50-kat5-2 3547951 N/A 3547953 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3547954 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 1f,1g gpr50-kat5-3 3547952 N/A 3547953 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3547954 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Figure 2a, 2b, 2c