IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhuo-2010-1 human-2BS human 2BS 2bs human fetal lung cell line 2BS spontaneously transformed human fetal lung cell line mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Zhuo de X., Niu XH., Chen YC., Xin DQ., Guo YL., Mao ZB. zbmao@bjmu.edu.cn The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation zhuo-2010-2 human-2BS human 2BS 2bs human fetal lung cell line 2BS spontaneously transformed human fetal lung cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Zhuo de X., Niu XH., Chen YC., Xin DQ., Guo YL., Mao ZB. zbmao@bjmu.edu.cn The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation hsa-mir-17-5p microRNA 17 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human CAAAGUGCUUACAGUGCAGGUAG mrna_vdup1 thioredoxin interacting protein TXNIP, vdup1, mRNA mrna messenger rna mRNA mrna human Homo sapiens Human GTGGCTCTTCTGGCCCGGGCTACTATATAGAGACGTTTCCGCCTCCTGCTTGAAACTAACCCCTCTTTTTCTCCAAAGGAGTGCTTGTGGAGATCGGATCTTTTCTCCAGCAATTGGGGGAAAGAAGGCTTTTTCTCTGAATTAGCTTAGTGTAACCAGCGGCGTATATTTTTTAGGCGCCTTTTCGAAAACCTAGTAGTTAATATTCATTTGTTTAAATCTTATTTTATTTTTAAGCTCAAACTGCTTAAGAATACCTTAATTCCTTAAAGTGAAATAATTTTTTGCAAAGGGGTTTCCTCGATTTGGAGCTTTTTTTTTCTTCCACCGTCATTTCTAACTCTTAAAACCAACTCAGTTCCATCATGGTGATGTTCAAGAAGATCAAGTCTTTTGAGGTGGTCTTTAACGACCCTGAAAAGGTGTACGGCAGTGGCGAGAAGGTGGCTGGCCGGGTGATAGTGGAGGTGTGTGAAGTTACTCGTGTCAAAGCCGTTAGGATCCTGGCTTGCGGAGTGGCTAAAGTGCTTTGGATGCAGGGATCCCAGCAGTGCAAACAGACTTCGGAGTACCTGCGCTATGAAGACACGCTTCTTCTGGAAGACCAGCCAACAGGTGAGAATGAGATGGTGATCATGAGACCTGGAAACAAATATGAGTACAAGTTCGGCTTTGAGCTTCCTCAGGGGCCTCTGGGAACATCCTTCAAAGGAAAATATGGGTGTGTAGACTACTGGGTGAAGGCTTTTCTTGACCGCCCGAGCCAGCCAACTCAAGAGACAAAGAAAAACTTTGAAGTAGTGGATCTGGTGGATGTCAATACCCCTGATTTAATGGCACCTGTGTCTGCTAAAAAAGAAAAGAAAGTTTCCTGCATGTTCATTCCTGATGGGCGGGTGTCTGTCTCTGCTCGAATTGACAGAAAAGGATTCTGTGAAGGTGATGAGATTTCCATCCATGCTGACTTTGAGAATACATGTTCCCGAATTGTGGTCCCCAAAGCTGCCATTGTGGCCCGCCACACTTACCTTGCCAATGGCCAGACCAAGGTGCTGACTCAGAAGTTGTCATCAGTCAGAGGCAATCATATTATCTCAGGGACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGCCTTCTATCCTGGGCTGCAACATCCTTCGAGTTGAATATTCCTTACTGATCTATGTTAGCGTTCCTGGATCCAAGAAGGTCATCCTTGACCTGCCCCTGGTAATTGGCAGCAGATCAGGTCTAAGCAGCAGAACATCCAGCATGGCCAGCCGAACCAGCTCTGAGATGAGTTGGGTAGATCTGAACATCCCTGATACCCCAGAAGCTCCTCCCTGCTATATGGATGTCATTCCTGAAGATCACCGATTGGAGAGCCCAACCACTCCTCTGCTAGATGACATGGATGGCTCTCAAGACAGCCCTATCTTTATGTATGCCCCTGAGTTCAAGTTCATGCCACCACCGACTTATACTGAGGTGGATCCCTGCATCCTCAACAACAATGTGCAGTGAGCATGTGGAAGAAAAGAAGCAGCTTTACCTACTTGTTTCTTTTTGTCTCTCTTCCTGGACACTCACTTTTTCAGAGACTCAACAGTCTCTGCAATGGAGTGTGGGTCCACCTTAGCCTCTGACTTCCTAATGTAGGAGGTGGTCAGCAGGCAATCTCCTGGGCCTTAAAGGATGCGGACTCATCCTCAGCCAGCGCCCATGTTGTGATACAGGGGTGTTTGTTGGATGGGTTTAAAAATAACTAGAAAAACTCAGGCCCATCCATTTTCTCAGATCTCCTTGAAAATTGAGGCCTTTTCGATAGTTTCGGGTCAGGTAAAAATGGCCTCCTGGCGTAAGCTTTTCAAGGTTTTTTGGAGGCTTTTTGTAAATTGTGATAGGAACTTTGGACCTTGAACTTACGTATCATGTGGAGAAGAGCCAATTTAACAAACTAGGAAGATGAAAAGGGAAATTGTGGCCAAAACTTTGGGAAAAGGAGGTTCTTAAAATCAGTGTTTCCCCTTTGTGCACTTGTAGAAAAAAAAGAAAAACCTTCTAGAGCTGATTTGATGGACAATGGAGAGAGCTTTCCCTGTGATTATAAAAAAGGAAGCTAGCTGCTCTACGGTCATCTTTGCTTAGAGTATACTTTAACCTGGCTTTTAAAGCAGTAGTAACTGCCCCACCAAAGGTCTTAAAAGCCATTTTTGGAGCCTATTGCACTGTGTTCTCCTACTGCAAATATTTTCATATGGGAGGATGGTTTTCTCTTCATGTAAGTCCTTGGAATTGATTCTAAGGTGATGTTCTTAGCACTTTAATTCCTGTCAAATTTTTTGTTCTCCCCTTCTGCCATCTTAAATGTAAGCTGAAACTGGTCTACTGTGTCTCTAGGGTTAAGCCAAAAGACAAAAAAAATTTTACTACTTTTGAGATTGCCCCAATGTACAGAATTATATAATTCTAACGCTTAAATCATGTGAAAGGGTTGCTGCTGTCAGCCTTGCCCACTGTGACTTCAAACCCAAGGAGGAACTCTTGATCAAGATGCCCAACCCTGTGATCAGAACCTCCAAATACTGCCATGAGAAACTAGAGGGCAGGTCTTCATAAAAGCCCTTTGAACCCCCTTCCTGCCCTGTGTTAGGAGATAGGGATATTGGCCCCTCACTGCAGCTGCCAGCACTTGGTCAGTCACTCTCAGCCATAGCACTTTGTTCACTGTCCTGTGTCAGAGCACTGAGCTCCACCCTTTTCTGAGAGTTATTACAGCCAGAAAGTGTGGGCTGAAGATGGTTGGTTTCATGTTTTTGTATTATGTATCTTTTTGTATGGTAAAGACTATATTTTGTACTTAACCAGATATATTTTTACCCCAGATGGGGATATTCTTTGTAAAAAATGAAAATAAAGTTTTTTTAATGGAAAAAAAAATGTCTGTGAAAAAAAAAAAAAAAAAA foxo3_human Forkhead box protein O3 FOXO3 FKHRL1 FOXO3A Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein protein protein human Homo sapiens Human MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG E5B4E830665A9982 txnip_human_gene thioredoxin interacting protein VDUP1 gene gene human Homo sapiens Human hsa_mir_17 5p-mrna_vdup1-1 3555169 N/A 3555171 regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified N/A 3555172 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag 3' range 3' range 3' range 3' range false r.[2337_2341delinsgagtc;2734_2738delinsgagta] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false down-regulates quantity by destabilization Fig 4 E, F The interaction occurs in senescent cells only foxo3-txnip_human_gene-1 3555170 N/A 3555173 antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3555174 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false fig 3 C The binding occurs in senescent cells only