DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gong-2018-3 mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. raozh@tsinghua.edu.cn,wangq@ibp.ac.cn,feisun@ibp.ac.cn Science (New York, N.Y.) 2018 Only protein-protein interactions imex curation imex curation Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z., Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. gong-2018-5 mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column fingerprinting peptide massfingerprinting fingerprinting Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. raozh@tsinghua.edu.cn,wangq@ibp.ac.cn,feisun@ibp.ac.cn Science (New York, N.Y.) 2018 Only protein-protein interactions imex curation imex curation Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z., Gong H., Li J., Xu A., Tang Y., Ji W., Gao R., Wang S., Yu L., Tian C., Li J., Yen HY., Man Lam S., Shui G., Yang X., Sun Y., Li X., Jia M., Yang C., Jiang B., Lou Z., Robinson CV., Wong LL., Guddat LW., Sun F., Wang Q., Rao Z. a0qvh4_mycs2 MSMEG_2575 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MANTEVERHTGVDVEDVPSAEWGWSHMPIGVMHIGGLLSAAFLLVMMRGNHVGHVEDWFLIGFAAVIVALVGRNWWLRRRGWIR a0qqq1_mycs2 Superoxide dismutase [Cu-Zn] sodC MSMEG_0835 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MLKPVSVAVLFATPVLALSACSPPGETASSEPGTTPAIWTGSPSPAAPSGEDHGGGHGAGAAGAGETLTAELKTADGTSVATADFQFADGFATVTIETTTPGRLTPGFHGVHIHSVGKCEANSVAPTGGAPGDFNSAGGHFQVSGHSGHPASGDLSSLQVRADGSGKLVTTTDAFTAEDLLDGAKTAIIIHEKADNFANIPPERYQQVNGAPGPDQTTMATGDAGSRVACGVISAG a0r562_mycs2 MSMEG_6078 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MNRFSSRAGLAVCGLATAVALTACSAGQISQTTTQEPAVNGVNAQAGQVSLRNVHLRAPQQTDYVEPGTTVELLFVAANDSTEGSNKLKSITSDVGEVTLTGDSTVPADGVLIVGEPDGQIQAVENAEAADAVTAEVELTKPITNGLLYDFTFTFEDGETTVAVPISAGEQPRRPVPPAGPGSSEH y4692_mycs2 Uncharacterized protein MSMEG_4692/MSMEI_4575 MSMEG_4692 MSMEI_4575 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MASGDIATVANAELDLPYGSALTSSGRISAVTEPGELSVHYPFPTMDLVVLDDALKYGSRAAKARFAVYIGPLGADTAATAREILANVPTPENAVLLAVSPDQRAIEVVYGADVKGRGIESAAPLGVSAAAASFKEGNLIDGLISAVRVMSAGVSPA a0r1b6_mycs2 MSMEG_4693 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSTALTHGLIGGVPLVLFAVLALIFLTRKGPHPDTYKMSDPWTHAPILWAAEEPREHGHGGHGHDSHGVVIGGGASGKW a0r056_mycs2 Cytochrome c oxidase polypeptide 4 Cytochrome aa3 subunit 4 Cytochrome c oxidase polypeptide IV MSMEG_4267 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MHIEARLFEILTAFFALAAVVYAVLTAMFATGGVEWAGTTALVLTTGLTLITGTFFRFVARRLDTRPEDYEDAEISDGAGELGFFAPHSWWPILISLSFSTAAVGAALWLPWLIAAGVAFVITSVCGLVFEYYWGPEKH a0r049_mycs2 Cytochrome aa3 subunit 3 Probable cytochrome c oxidase subunit 3 MSMEG_4260 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTSAVGTSGTAITSRVHSLNRPNMVSVGTIVWLSSELMFFAGLFAMYFTARAQAGGAWPPEPTELNLALAVPVTLVLIASSFTCQMGVFAAERGDVFGLRRWYVITFLMGLFFVLGQGYEYIHLVEHGTTIPGSAYGSVFYLATGFHGLHVIGGLVAFVLLLARTKMSKFTPAQATAAIVVSYYWHFVDIVWIALFATIYFVR a0a8b4r8z7_mycsm ctaD_2 NCTC7017_06542 protein protein mycsm Mycobacterium smegmatis MGPKGNLIYKLITTTDHKLIGIMYCVVCFAFFLVGGLMALFMRTELAMPGLQFLSNEQFNQLFTMHGTVMLLFYATPIVFGFANLVLPLQIGAPDVAFPRLNALSFWLFLFGALIAIAGFITPGGAADFGWTAYSPLTDAIHSPGAGGDLWIMGLAVGGLGTILGGVNMITTVVCMRAPGMTMFRMPIFTWNILVTSILVLIAFPILTAALFGLAADRHLGAHIYDPANGGVLLWQHLFWFFGHPEVYIIALPFFGIVSEIFPVFSRKPIFGYTTLIYATLAIAALSVAVWAHHMYATGAVLLPFFSFMTFLIAVPTGIKFFNWIGTMWKGQLTFETPMLFSVGFLITFLLGGLSGVLLASPPLDFHVTDSYFVIAHFHYVLFGTIVFATYAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQHWLGDEGMPRRYADYLPTDGFTTLNVISTVGAFILGVSMLPFVWNVFKSWRYGEPVTVDDPWGYGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMVERMRAEAHVGRAHHPELETADKSS a0r057_mycs2 Cytochrome aa3 subunit 2 MSMEG_4268 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTPRGFRVVALSIVLGGSALLLSGCSWSDALALGWPTGITPEAKLNRELWIGSVIASFAVGAIVWGLIFWTSAFHRKKATDTELPRQFGYNMPLELTLTVIPFLIISVLFYFTVVVQERMMHKDPNPEVVIDVTAFQWNWKFGYQKIAFADGSFDYDGADPERKEAMTSRPEGKDEHGIEKVGPIRGMTPEDRTYLNFDKIETLGTSSEIPVLVLPAGKRIEFVLNSADVIHGFWVPEFLFKRDVLPEPKANNSDNVFQVSEIQQTGAFVGRCTEMCGTFHAMMNFEVRVVEPNDFKAYIDQRNAGKTNAEALAAINQPPLAITTEPFESRRGELVPQASK i7fph1_mycs2 Cytochrome bc1 complex cytochrome c subunit qcrC MSMEI_4161 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MDRAVRHHLLRPMNIGSPMSSMRRGSMTSKSRRRLRRRLSAGLLLLIGLAVAGGVAATLTPQPQVAVADESQSALLRTGKQLFETSCVSCHGANLQGVPDRGPSLIGTGEAAVYFQVSTGRMPAMRGEAQAPSKPPHFDESQIDALGAYVQANGGGPTVPRDDHGAVAQESLIGGDVARGGDLFRLNCASCHNFTGKGGALSSGKYAPDLGDANPAQIYTAMLTGPQNMPKFSDRQLTPDEKRDIVAYVRESAETPSYGGYGLGGFGPAPEGMAMWIIGMVAAIGVAMWIGSRA a0r052_mycs2 Cytochrome bc1 complex cytochrome b subunit Cytochrome bc1 reductase complex subunit QcrB MSMEG_4263 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSPDFAKLAAAQGDAIDSRYHPSAAVRRQLNKVFPTHWSFLLGEIALYSFIILLLTGVWLTLFFDPSMAHVTYDGVYQPLRGVQMSRAYETALDISFEVRGGLFVRQVHHWAALMFAASIMVHLARIFFTGAFRRPREANWVIGSLLLILAMFEGFFGYSLPDDLLSGTGIRAALSGITMGIPVIGTWMHWALFGGDFPGEILIPRLYALHILLIPGIILALIGAHLALVWFQKHTQFPGPGRTETNVVGVRVMPVFAVKSGAFFAMITGVLGLMGGLLTINPIWNLGPYKPSQVSAGSQPDFYMMWTDGLIRLWPAWEFYPFGHTIPQGVWVAVGMGLVFALLIAYPFIEKKVTGDDAHHNLLQRPRDVPVRTAIGSMAIALYLLLTFACMNDIIALKFHISLNATTWIGRIGMVVLPAIVYFVAYRWAISLQRSDREVLEHGVETGIIKRLPHGAYVELHQPLGPVDEHGHPIPLEYAGAPLPKRMNKLGSGGAPGTGSFLFPDPAVEHEALTEAAHASEHKSLTALKEHQDRIHGNGETNGHH i7gd61_mycs2 Cytochrome bc1 complex Rieske iron-sulfur subunit qcrA Cytochrome bc1 reductase complex subunit QcrA MSMEI_4162 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MDYRNGGRHRCGHVDRIASMSQDSPDIKGTDAPGQTGVPGQPTDAELAEMSREELVKLGGKIDGVETIFKEPRWPVPGTKAEKRTERLVAYWLMLGGLSGLALLLVFLFWPWEYQPFGSEGEFLYSLATPLYGLTFGLSILSIGIGAVLFQKKFIPEEISVQDRHDGRSPEVHRKTVAANLTDALEGSTLKRRKVIGLSLGIGLGAFGAGTLVAFIGGLIKNPWKPVVPTAEGKKAVLWTSGWTPRFKGETIYLARATGRPGESPFVKMRPEDIDAGGMETVFPWRESDGDGTTVESEHKLTEIAMGVRNPVMLIRIKPADMHRVIKRKGQESFNFGELFAYTKVCSHLGCPSSLYEQQTYRILCPCHQSQFDALEFAKPIFGPAARALAQLPITIDEDGYLVANGDFVEPVGPAFWERKS a0qr51_mycs2 MSMEG_0987 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MRHRLERTAVPYVSYVLVLVGFVAFGALVASLAYGSHIPAGIAGAVLAGSLTGAVIGFRAAARRLRADRGDAHNVSIFDTPLEPEMVDRYARRYRATGTDAEESAVAVLPAAETAVARERRAA a0qpw0_mycs2 MSMEG_0537 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MAAERKKDRPNLLQYIAYSYGRRLPDSMREWVAHDLADHGAVRRHMIRMAIPPALVLAPFWLLPASLYVHIEMTVPIYIWSLLMALALNKVWRRHRLAQHGLDPNLVDEIRYKKQAHIHEDYIRRFGPRPESAKFQSNSSPF a0qvz6_mycs2 MSMEG_2753 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MKQSHELSFDDDFDAEARPVLITRAAPAYEVQHRQRVRKYLTLMAFRIPALILAAVAYNIWENGLISLAIIAVSIPLPWMAVLIANDRPPRKREEPRRYAGRGEKIPLFPTAERPALMSDLHREPEAPPQPGTADFRGHPAS i7fph7_mycs2 Cytochrome c oxidase polypeptide 4 Cytochrome aa3 subunit 4 Cytochrome c oxidase polypeptide IV MSMEI_4166 ctaF protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MHIEARLFEILTAFFALAAVVYAVLTAMFATGGVEWAGTTALVLTTGLTLITGTFFRFVARRLDTRPEDYEDAEISDGAGELGFFAPHSWWPILISLSFSTAAVGAALWLPWLIAAGVAFVITSVCGLVFEYYWGPEKH i7g4q8_mycs2 Cytochrome aa3 subunit 3 ctaE Probable cytochrome c oxidase subunit 3 MSMEI_4160 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTSAVGTSGTAITSRVHSLNRPNMVSVGTIVWLSSELMFFAGLFAMYFTARAQAGGAWPPEPTELNLALAVPVTLVLIASSFTCQMGVFAAERGDVFGLRRWYVITFLMGLFFVLGQGYEYIHLVEHGTTIPGSAYGSVFYLATGFHGLHVIGGLVAFVLLLARTKMSKFTPAQATAAIVVSYYWHFVDIVWIALFATIYFVR i7gd63_mycs2 Cytochrome aa3 subunit 2 ctaC MSMEI_4167 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MTPRGFRVVALSIVLGGSALLLSGCSWSDALALGWPTGITPEAKLNRELWIGSVIASFAVGAIVWGLIFWTSAFHRKKATDTELPRQFGYNMPLELTLTVIPFLIISVLFYFTVVVQERMMHKDPNPEVVIDVTAFQWNWKFGYQKIAFADGSFDYDGADPERKEAMTSRPEGKDEHGIEKVGPIRGMTPEDRTYLNFDKIETLGTSSEIPVLVLPAGKRIEFVLNSADVIHGFWVPEFLFKRDVLPEPKANNSDNVFQVSEIQQTGAFVGRCTEMCGTFHAMMNFEVRVVEPNDFKAYIDQRNAGKTNAEALAAINQPPLAITTEPFESRRGELVPQASK a0a8b4r3r6_mycsm qcrC NCTC7017_04661 protein protein mycsm Mycobacterium smegmatis MTSKSRRRLRRRLSAGLLLLIGLAVAGGVAATLTPQPQVAVADESQSALLRTGKQLFETSCVSCHGANLQGVPDRGPSLIGTGEAAVYFQVSTGRMPAMRGEAQAPSKPPHFDESQIDALGAYVQANGGGPTVPRDDHGAVAQESLIGGDVARGGDLFRLNCASCHNFTGKGGALSSGKYAPDLGDANPAQIYTAMLTGPQNMPKFSDRQLTPDEKRDIVAYVRESAETPSYGGYGLGGFGPAPEGMAMWIIGMVAAIGVAMWIGSRA i7fgs8_mycs2 Cytochrome bc1 complex cytochrome b subunit qcrB Cytochrome bc1 reductase complex subunit QcrB MSMEI_4163 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSPDFAKLAAAQGDAIDSRYHPSAAVRRQLNKVFPTHWSFLLGEIALYSFIILLLTGVWLTLFFDPSMAHVTYDGVYQPLRGVQMSRAYETALDISFEVRGGLFVRQVHHWAALMFAASIMVHLARIFFTGAFRRPREANWVIGSLLLILAMFEGFFGYSLPDDLLSGTGIRAALSGITMGIPVIGTWMHWALFGGDFPGEILIPRLYALHILLIPGIILALIGAHLALVWFQKHTQFPGPGRTETNVVGVRVMPVFAVKSGAFFAMITGVLGLMGGLLTINPIWNLGPYKPSQVSAGSQPDFYMMWTDGLIRLWPAWEFYPFGHTIPQGVWVAVGMGLVFALLIAYPFIEKKVTGDDAHHNLLQRPRDVPVRTAIGSMAIALYLLLTFACMNDIIALKFHISLNATTWIGRIGMVVLPAIVYFVAYRWAISLQRSDREVLEHGVETGIIKRLPHGAYVELHQPLGPVDEHGHPIPLEYAGAPLPKRMNKLGSGGAPGTGSFLFPDPAVEHEALTEAAHASEHKSLTALKEHQDRIHGNGETNGHH ctad cytochrome c oxidase subunit I protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MVAEAPPIGELEARRPFPERMGPKGNLIYKLITTTDHKLIGIMYCVVCFAFFLVGGLMALFMRTELAMPGLQFLSNEQFNQLFTMHGTVMLLFYATPIVFGFANLVLPLQIGAPDVAFPRLNALSFWLFLFGALIAIAGFITPGGAADFGWTAYSPLTDAIHSPGAGGDLWIMGLAVGGLGTILGGVNMITTVVCMRAPGMTMFRMPIFTWNILVTSILVLIAFPILTAALFGLAADRHLGAHIYDPANGGVLLWQHLFWFFGHPEVYIIALPFFGIVSEIFPVFSRKPIFGYTTLIYATLAIAALSVAVWAHHMYATGAVLLPFFSFMTFLIAVPTGIKFFNWIGTMWKGQLTFETPMLFSVGFLITFLLGGLSGVLLASPPLDFHVTDSYFVIAHFHYVLFGTIVFATYAGIYFWFPKMTGRLLDERLGKLHFWLTFIGFHTTFLVQHWLGDEGMPRRYADYLPTDGFTTLNVISTVGAFILGVSMLPFVWNVFKSWRYGEPVTVDDPWGYGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHMVERMRAEAHVGRAHHPELETADKSS a0r049_mycs2-a0r052_mycs2-1 3494011 N/A 3494013 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494014 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494015 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494016 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494017 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494018 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494019 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494020 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494021 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494022 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 N/A 3494023 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified HIS tag HIS tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Stoichiometry: 2.0 N/A 3494024 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 physical association physical association false false false Fig 1B, Table S2, Fig S3A-G, movie S1 a0r562_mycs2-ctac-1 3494012 N/A 3494025 unspecified role unspecified role neutral component neutral component purified purified N/A 3494026 unspecified role unspecified role neutral component neutral component purified purified N/A 3494027 unspecified role unspecified role neutral component neutral component purified purified N/A 3494017 unspecified role unspecified role neutral component neutral component purified purified N/A 3494015 unspecified role unspecified role neutral component neutral component purified purified N/A 3494014 unspecified role unspecified role neutral component neutral component purified purified N/A 3494016 unspecified role unspecified role neutral component neutral component purified purified N/A 3494028 unspecified role unspecified role neutral component neutral component purified purified N/A 3494029 unspecified role unspecified role neutral component neutral component purified purified N/A 3494030 unspecified role unspecified role neutral component neutral component purified purified N/A 3494031 unspecified role unspecified role neutral component neutral component purified purified N/A 3494032 unspecified role unspecified role neutral component neutral component purified purified N/A 3494024 unspecified role unspecified role neutral component neutral component purified purified N/A 3494013 unspecified role unspecified role neutral component neutral component purified purified N/A 3494033 unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false Fig S1D, Table S1