DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kovtun-2018-1 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Kovtun O., Leneva N., Bykov YS., Ariotti N., Teasdale RD., Schaffer M., Engel BD., Owen DJ., Briggs JAG., Collins BM. b.collins@imb.uq.edu.au Nature 2018 imex curation Only protein-protein interactions imex curation kovtun-2018-2 ecoli Escherichia coli (strain K12) sls static light scattering sls weight by comassie molecular weight estimation by coomasie staining weight by comassie Kovtun O., Leneva N., Bykov YS., Ariotti N., Teasdale RD., Schaffer M., Engel BD., Owen DJ., Briggs JAG., Collins BM. b.collins@imb.uq.edu.au Nature 2018 imex curation Only protein-protein interactions imex curation kovtun-2018-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kovtun O., Leneva N., Bykov YS., Ariotti N., Teasdale RD., Schaffer M., Engel BD., Owen DJ., Briggs JAG., Collins BM. b.collins@imb.uq.edu.au Nature 2018 imex curation Only protein-protein interactions imex curation kovtun-2018-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kovtun O., Leneva N., Bykov YS., Ariotti N., Teasdale RD., Schaffer M., Engel BD., Owen DJ., Briggs JAG., Collins BM. b.collins@imb.uq.edu.au Nature 2018 imex curation Only protein-protein interactions imex curation kovtun-2018-5 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kovtun O., Leneva N., Bykov YS., Ariotti N., Teasdale RD., Schaffer M., Engel BD., Owen DJ., Briggs JAG., Collins BM. b.collins@imb.uq.edu.au Nature 2018 imex curation Only protein-protein interactions imex curation g0s0e6_chatd CTHT_0009750 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MSYFFSTPVDIDIVLADADKRAMVDVKLDKNRREKVPLYMDGESVKGCVTVRPKDGKRLEHTGIKVQFIGTIEMFFDRGNHYEFLSLVQELAAPGELQHPQTFDFNFKNVEKQYESYNGINVKLRYFVRVTVSRRMADVIREKDIWVYSYRIPPELNSSIKMDVGIEDCLHIEFEYSKSKYHLKDVIVGRIYFLLVRLKIKHMELSIIRRETTGVAPNQYNESETLVRFEIMDGSPSRGETIPIRLFLGGFDLTPTFRDVNKKFSTRYYLSLVLIDEDARRYFKQSEIILYRQPPEYDNQLLPPPPDQKQVTAPLA g0s709_chatd Vacuolar protein sorting-associated protein 35 CTHT_0035730 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MSTPAPPEEQARLLEDALIAVRQQTAMMRKFLDTPGKLMDALKCCSTLVSELRTSSLSPKQYYELYMAVFDALRYLSAHLRENHPVNHLADLYELVQYAGNIIPRLYLMITVGTAYMSIDGAPVKELMKDMMDMSRGVQHPVRGLFLRYYLSGQARDYLPTGDSDGPEGNLQDSINFILTNFVEMNKLWVRLQHQGHSRERDLRTQERRELQLLVGSNIVRLSQLVDLPTYRDSILGPLLEQIVQCRDILAQEYLLEVITQVFPDEYHLHTLDQFLGAVSRLNPHVNVKAIVIGMMNRLSDYAERESQNEPEEDRAKLEEEALAKLLEKTKLGQNSELEPQNGDHPDTEVSSTTDSAQAPSTADSDTTAVNGEEEPVRKRRGIPVNVPLYDIFFDQVQHLVQAQHLPIQDTIALCCSLANLSLNIYPERLDYVDGILAYALAKVKEHANSADLHSQPAQQSLLSLLQSPLRRYVSIFTALSLPTYVSLFQAQTYPTRRAIAGEIVRTLLKNQTLISTPAHLENVLEILKVLIKEGSQPPAGYPGVVQPRARPLETDETMEEQGWLARLVHLIHSDDNDTQFRLLQMTRKAYAEGNERIRTTTPPLITAGLKLARRFKAREHYDDNWSSQSSSLFKFLHSAISTLYTRVNGPGVADLCLRLFCSCGQVADMTEFEEVAYEFFAQAFTVYEESISDSKAQFQAVCVIASALHRTRNFGRENYDTLITKCAQHASKLLRKPDQCRAVYLASHLWWATPIAARGETEDTELYRDGKRVLECLQRALRVADSCMETATSIELFVEILDRYVYYFDQRNESVTTKYLNGLIELIHSNLAGNQQDSASVEASRKHFIQTLEMIQSKEFEGIVVAPK g0rzb5_chatd Vacuolar protein sorting-associated protein 29 CTHT_0002370 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MAFLILVIGNLHIPDRALDIPPKFKKLLSPGKISQTLCLGNLTDRATYDYLRSISPDLKIVRGRMDVEATSLPLMQVVTHGSLRIGFLEGFTLVSEEPDVLLAEANKLDVDVLCWAGGSHRFECFEYMDKFFVNPGSATGAFTTDWLAEGEEVVPSFCLMDVQGISLTLYVYQLRKDENGTENVAVEKVTYTKPVEPTGAS g0sh11_chatd CTHT_0068290 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MEDPWADTASPNSSAPLSQHVEDSSSAPAAAAETPSNTATTTAPSISTSSRPSRATPRRIVAKPKSLEAVEDDPLGPLGAPTPSSDSDNNGLGGATARGVQPPVPPLKEQLPLRTTLPPSTTNRGGRGRRSGPPDPHHIDDDDDDAALFGSAGRGRPPPPVPASLPSPVKSSTAPSMSVEQAARPTFHITVGDPHKVGDLATSHIVYSVRTKTTSKAYKQPEFEVKRRYRDFLWLYNTLHSNNPGVVVPPPPEKQAVGRFESNFVESRRAALEKMLNKIAAHPTLQLDADLKLFLESESFNIDVKHKERKEPPLGESKGVFGSLGFGGGGNKFVEQDDWFHDRRVYLDALENQLKALLKAMDNMVAQRKAMAEAAADFSASLHALSTVELSPTLSGPLDALSELQLAIRDVYERQAQQDVLTFGIIIEEYIRLIGSVKQAFSQRQKAFHSWHSAESELMKKKAAQDKLLRQGKTQQDRLNQVNAEVIDAERKVHQARLLFEDMGRLLRSELDRFEREKVEDFKSGVETFLESAVEAQKELIEKWETFLMQLDAEDDESVFYRPPPVQTKKPAGDTAVDRARARIDEDSD g0rzb5_chatd-g0s0e6_chatd-1 3487977 N/A 3487982 predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3487983 predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3487984 predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) N/A 3487985 predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 1a, Extended Data Fig 1a-d, Extended Data Table 1 g0sh11_chatd-1 3487978 N/A 3487985 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Extended Data Fig 1c g0rzb5_chatd-1 3487979 N/A 3487984 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3487983 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3487982 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3487985 unspecified role unspecified role bait bait purified purified GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) association association false false false Extended Data Fig 1d g0rzb5_chatd-g0s0e6_chatd-2 3487980 N/A 3487984 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3487983 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 3487982 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) physical association physical association false false false Extended Data Fig 1a g0sh11_chatd-2 3487981 N/A 3487985 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Extended Data Fig 1b