UniProt UniProt http://www.uniprot.org ciccone-2018-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ciccone L., Fruchart-Gaillard C., Mourier G., Savko M., Nencetti S., Orlandini E., Servent D., Stura EA., Shepard W. Alzheimers - Interactions investigated in the context of Alzheimers disease william.shepard@synchrotron-soleil.fr Scientific reports 2018 imex curation Only protein-protein interactions imex curation ciccone-2018-1 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Ciccone L., Fruchart-Gaillard C., Mourier G., Savko M., Nencetti S., Orlandini E., Servent D., Stura EA., Shepard W. Alzheimers - Interactions investigated in the context of Alzheimers disease william.shepard@synchrotron-soleil.fr Scientific reports 2018 imex curation Only protein-protein interactions imex curation copper(2+) Copper (Cu2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) tthy_human Transthyretin Prealbumin TBPA ATTR TTR PALB protein protein human Homo sapiens Human MASHRLLLLCLAGLVFVSEAGPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 manganese(2+) Manganese (2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) iron(3+) iron(3+) FE (III) ION Fe(III) Fe3+ Fe(3+) Ferric ion ferric iron Iron(3+) iron, ion (Fe(3+)) iron(III) cation iron(3+) iron(3+) ion small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) app-ttr-2 3487700 N/A 3487702 unspecified role unspecified role neutral component neutral component N/A 3487703 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 N/A 3487704 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Table 1 2.14A copper(2+)-ttr-1 3487700 N/A 3487702 unspecified role unspecified role neutral component neutral component Stoichiometry: 3.0 N/A 3487703 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 direct interaction direct interaction false false false Figs 1B, 1C manganese(2+)-ttr-1 3487700 N/A 3487705 unspecified role unspecified role neutral component neutral component Stoichiometry: 2.0 N/A 3487703 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 direct interaction direct interaction false false false Figs 1B, 1C iron2plus-ttr-1 3487700 N/A 3487706 unspecified role unspecified role neutral component neutral component Stoichiometry: 5.0 N/A 3487703 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 4.0 direct interaction direct interaction false false false Crystals were soaked with Cu2+ (5N7C), Mn2+ (5N62) or Fe2+ (5N5Q) Figs 1B, 1C app-ttr-1 3487701 N/A 3487703 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3487704 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Binding is copper-dependent Figs 1B, 1C Copper 2+ (CHEBI:29036)