DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ walsh-2018-1 human-293s Human 293S cell line Human 293s 293S cell line 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Walsh RM., Roh SH., Gharpure A., Morales-Perez CL., Teng J., Hibbs RE., Walsh RM., Roh SH., Gharpure A., Morales-Perez CL., Teng J., Hibbs RE. ryan.hibbs@utsouthwestern.edu Nature 2018 imex curation Only protein-protein interactions imex curation walsh-2018-2 human-293s Human 293S cell line Human 293s 293S cell line molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Walsh RM., Roh SH., Gharpure A., Morales-Perez CL., Teng J., Hibbs RE., Walsh RM., Roh SH., Gharpure A., Morales-Perez CL., Teng J., Hibbs RE. ryan.hibbs@utsouthwestern.edu Nature 2018 imex curation Only protein-protein interactions imex curation p43681-1 Neuronal acetylcholine receptor subunit alpha-4 CHRNA4 NACRA4 protein protein human Homo sapiens Human MELGGPGAPRLLPPLLLLLGTGLLRASSHVETRAHAEERLLKKLFSGYNKWSRPVANISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPADYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFHDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVNMHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPTWVRRVFLDIVPRLLLMKRPSVVKDNCRRLIESMHKMASAPRFWPEPEGEPPATSGTQSLHPPSPSFCVPLDVPAEPGPSCKSPSDQLPPQQPLEAEKASPHPSPGPCRPPHGTQAPGLAKARSLSVQHMSSPGEAVEGGVRCRSRSIQYCVPRDDAAPEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI achb2_human Neuronal acetylcholine receptor subunit beta-2 CHRNB2 protein protein human Homo sapiens Human MARRCGPVALLLGFGLLRLCSGVWGTDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEVASLDDFTPSGEWDIVALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPALLFMQQPRHHCARQRLRLRRRQREREGAGALFFREAPGADSCTCFVNRASVQGLAGAFGAEPAPVAGPGRSGEPCGCGLREAVDGVRFIADHMRSEDDDQSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLFQNYTTTTFLHSDHSAPSSK chrna4-chrnb2-3 3468594 N/A 3468596 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P43681-1:p.Phe585_Ser586insGluArg Glu-Arg Insertion mutation mutation certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human Stoichiometry: 2.0 N/A 3468597 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P17787:p.Gln445_Ser446insGluArg Glu-Arg Insertion mutation mutation certain certain sequence position certain certain certain sequence position certain false strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human Stoichiometry: 3.0 physical association physical association false false false Figure 1 chrna4-chrnb2-4 3468594 N/A 3468596 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false p.Phe585_Ser586insGluArg Glu-Arg Linker cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human Stoichiometry: 3.0 N/A 3468597 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P17787:p.Gln445_Ser446insGluArg Glu-Arg Insertion mutation mutation certain certain sequence position certain certain certain sequence position certain false strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human Stoichiometry: 2.0 physical association physical association false false false Figure 1 chrna4-chrnb2-5 3468595 N/A 3468596 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P43681-1:p.Phe585_Ser586insGluArg Glu-Arg Insertion mutation mutation certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3468597 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P17787:p.Gln445_Ser446insGluArg Glu-Arg Insertion mutation mutation certain certain sequence position certain certain certain sequence position certain false strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false Figure 1 chrna4-chrnb2-6 3468595 N/A 3468596 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P43681-1:p.Phe585_Ser586insGluArg Glu-Arg Insertion mutation mutation certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3468597 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P17787:p.Gln445_Ser446insGluArg Glu-Arg Insertion mutation mutation certain certain sequence position certain certain certain sequence position certain false strep tag strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false Figure 1