DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gruszczyk-2018-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Gruszczyk J., Kanjee U., Chan LJ., Menant S., Malleret B., Lim NTY., Schmidt CQ., Mok YF., Lin KM., Pearson RD., Rangel G., Smith BJ., Call MJ., Weekes MP., Griffin MDW., Murphy JM., Abraham J., Sriprawat K., Menezes MJ., Ferreira MU., Russell B., Renia L., Duraisingh MT., Tham WH. tham@wehi.edu.au Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation gruszczyk-2018-5 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Gruszczyk J., Kanjee U., Chan LJ., Menant S., Malleret B., Lim NTY., Schmidt CQ., Mok YF., Lin KM., Pearson RD., Rangel G., Smith BJ., Call MJ., Weekes MP., Griffin MDW., Murphy JM., Abraham J., Sriprawat K., Menezes MJ., Ferreira MU., Russell B., Renia L., Duraisingh MT., Tham WH. tham@wehi.edu.au Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation gruszczyk-2018-1 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Gruszczyk J., Kanjee U., Chan LJ., Menant S., Malleret B., Lim NTY., Schmidt CQ., Mok YF., Lin KM., Pearson RD., Rangel G., Smith BJ., Call MJ., Weekes MP., Griffin MDW., Murphy JM., Abraham J., Sriprawat K., Menezes MJ., Ferreira MU., Russell B., Renia L., Duraisingh MT., Tham WH. tham@wehi.edu.au Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation gruszczyk-2018-2 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Gruszczyk J., Kanjee U., Chan LJ., Menant S., Malleret B., Lim NTY., Schmidt CQ., Mok YF., Lin KM., Pearson RD., Rangel G., Smith BJ., Call MJ., Weekes MP., Griffin MDW., Murphy JM., Abraham J., Sriprawat K., Menezes MJ., Ferreira MU., Russell B., Renia L., Duraisingh MT., Tham WH. tham@wehi.edu.au Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation gruszczyk-2018-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Gruszczyk J., Kanjee U., Chan LJ., Menant S., Malleret B., Lim NTY., Schmidt CQ., Mok YF., Lin KM., Pearson RD., Rangel G., Smith BJ., Call MJ., Weekes MP., Griffin MDW., Murphy JM., Abraham J., Sriprawat K., Menezes MJ., Ferreira MU., Russell B., Renia L., Duraisingh MT., Tham WH. tham@wehi.edu.au Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation trfe_human Serotransferrin Siderophilin Beta-1 metal-binding globulin TF PRO1400 protein protein human Homo sapiens Human MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP tfr1_human Transferrin receptor protein 1 TFRC T9 p90 protein protein human Homo sapiens Human MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF a5k736_plavs PVX_094255 protein protein Plasmodium vivax (strain Salvador I) Plasmodium vivax (strain Salvador I) MHIPIQPSPESTQITNTTDNIDYFDISDESNYYLISQLRPHFSNIYFFDEFKRYASYHTEIKRYEDIHKTKVNSLLNEASRAIGICNRAKNTVKGLINILENPQKFKTQRESYDVKLRQYEEKKEAFRGCLLNKNRKNLDQIKKINNEIRDLLEKLKCSQDCQTNVYFDMIKIYLVDFKKMPYENYDTFIKQYKNSYLSGVDMIRKIEKQIDNPVTINAIKFTQKEMGYIIDRFEYHLQKVKHSIDQVTALSDGVKPKQVTKNRLKEYYFNIGNYYSIFKFGKDSLNMLNKALIHKEKIVHNLLGELFGHLEERISKLIDSEYFITESNNIISQSEETLKLAEDVYDKNTKLIEDLTLYPHLEINEFKKDYDNNVEDLRESIIYIQSYVSSIKSAYRYNVLEKDSVESKQKNIPANSNAQKKVDELLSIIDSISYSNFSVAENFQKMKDYYKEIEKLKIKILQLIEAIKKYQQHVEELINKEKAVAILKEDINKIIEYIKGIIEKLKQLISANKDFDKIFQQVEQLINEALFNKDQFEHNKNDLHTKMKEIMHTFHERDLQQFLDNMSKFLKDQEASYQNADSKEKLDQLLTTVKAKQDELKEMKCDDIPDIIDNLKKESQNVLNLKDEVINKQFENMRTEMSSSLDQMTKEYNALKSSIEEYEAEKKGIENHKQNIIKRKNTFIVAEHENDEDVPEGKNTYNEFISNKDTILQKESAISNQMNTLEEKKRNRKTTLQTYGDAIQKLETYTEKKDEETKVLLDKFNTEVENFKLDEDEKSFNDAKSIVSNTINEVENENKNIDSIKKVNIAMKRSRSNQQSVEQLIRNKDNLLKEKMEKHFQKINTDEIIGQSAKANLLNNLESEKAIINEQLNDAHLNDLKLQIEKTLSYCEKSRENIKGNNGTHPEELNASEIEWEATKGKIETLNSNYQALNKNMDDIINKQHDEMIVLIDRDIKEKGKDIELKVEQRIQALEKMKAKLSSFDTNEDVKKYQSDINKEELIKLKEMSTAVLNKIDQNKNQLINVKTKSSGYVAESNGIKNKQTEPSQEKDKQIVYNTNRGDIEKVYKNIRDMYNELDDVEKEETTSNEVDNIEMQYDRLLINLLVQKINEENTKAKGLMEEIKSYIENIESVQAKMSDETREGATEFEFNQYQQSSLANDKKITEIVQHAINKKGEAERSKKTNEVKSIKNDVNTYMQQVIDEYNAMQKALGDIKNVNDLLISTSGRRIAEEISKSTNEAENFEKQAKGELGKINDIIEKMENQMVQAKEHENKIHINLEDKQIDEEVQKIRQIHEELINTKIAMNEHLSKTKAAKENCLSQVRNAERGKIKIDFLNNNKQSSNLKDVNMDHVTKNIADCVEHSKEVNKTENEAEKEHDLFLEYEKDITSILNRSSILGIETKSKKRKNEATKIMGQIEGEHSIIKTELQQSSENFKQLNEKYNLSEEKSMLANPTSTKANESIQYNIGKVKQNLLYIDNIAKEVDTILTNAQDSIRSISQISKLDGRNTLQMVEKEAADYVKYLDKITKEKELMNDKHKDLNPIKSDIANIETELKKHRKLFEIGLLNKVIEIAKTRKLYMDSVEQLLNSSINNFVTLFNGFNLKKYKIETSLDVYKGKISEFHTEFLQPYESIQNKEKRVLDPSVDFSAAKNMREEAQKEEKNIENVEKKTKKYLSDINKMESFTFIRNMRQTLDELSKTCKQEYAEVEGGHKDIEKIIEDIKSLNDESGSSAKLQQAEDKNSQMKAKVNHYSLKKEAHTILEQIVKSANFVGITIVTALQPSDLAAKASLQKAPELQFESQSKVTLESGQFLEKKNQLDVHENVQAAYNTVLQIYKYSDDIVRKQEKCEQLVKDGKDICLKFKSINEIKVMIQNSKGKESTLSAKVSHSFNKLSELNKIKCNDESYDAILETPSREELNKLRSTFKQEKDTIANQAKLSGYKTDFETHIGKLNDLAKIVDNLKASETLPKNIEEKKTSINLISTKLETIEKEIESINSSFDQLLEKGKKCEMTKYKLVRDSLSTKINDHSAIIKDNQKKATEYLTYIQNNHISIFKDIDMLNENLGEKSVSRYAIAKIEEANDLSAQLTAAVSEYEAIANSIRKEFTNISDHTEMDTLENEAKMLKEHYDNLINKKNIITELHNKINLIKLLEIRATSDKYVDIAELLGEVVKDQKKKLQEAKNKLDTLKDHIAVKEKELINHDSSFTLVSIKAFDEIYDDIKYNVGQLHTLEVTNFDELKKGKTYEENVTHLLNRRETLQNDLHNYEEKDKLKNTNIEMSNEENNQIRQTSEVIKKLESEFQNLLKIIQQSNTLCSNDNIKQFISDILKKVETIRERFVKNFPEREKYHQIEINYNEIKGIVKEVDTNPEISIFTEKINTYIRQKIRSAHHLEDAQKIKDIIEDVTSNYRKIKSKLSQVNNALDRIKIKKSEMDTLFESLSKENANNYNSAKYFLVDSDKIIKHLEDQVSKMSSLISYAEREIKELEEKVYSILNRPVIDNSASEQIKLLSDTQENDTDEAQKREFAEEQILNLPKQQIHSDEGNNNDMSTTDSANEEKQTGEEESQHEDQSQPRGIHSRVRLAEGIILGLSVFSGIIFISFRHKDEEKKDPESVGEQFEGNNNFNEDDKEEVIDVCFDNSDYSE a5k736_plavs-tf-2 3451178 N/A 3451183 unspecified role unspecified role neutral component neutral component purified purified human-serum Homo sapiens serum Serum N/A 3451184 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. N/A 3451185 unspecified role unspecified role neutral component neutral component purified purified necessary for binding necessary for binding required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 3C & Fig 3D a5k736_plavs-tf-9 3451178 N/A 3451183 unspecified role unspecified role neutral component neutral component purified purified human-serum Homo sapiens serum Serum N/A 3451184 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P02786:p.Gly217del p.Gly217del mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. N/A 3451185 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig 3C & Fig 4B tf-tfrc-8 3451178 N/A 3451183 unspecified role unspecified role neutral component neutral component purified purified human-serum Homo sapiens serum Serum N/A 3451184 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. direct interaction direct interaction false false false Fig 3C & Fig 3D tfrc-4 3451179 N/A 3451184 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 3E tf-tfrc-7 3451179 N/A 3451183 unspecified role unspecified role neutral component neutral component purified purified human Homo sapiens Human N/A 3451184 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. direct interaction direct interaction false false false Fig 3F a5k736_plavs-tf-4 3451179 N/A 3451185 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3451183 unspecified role unspecified role neutral component neutral component purified purified human Homo sapiens Human Stoichiometry: 1.0 N/A 3451184 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. Stoichiometry: 1.0 physical association physical association false false false Fig 3E & Fig 3F a5k736_plavs-tf-1 3451180 N/A 3451183 unspecified role unspecified role prey prey purified purified human Homo sapiens Human N/A 3451184 unspecified role unspecified role prey prey purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. N/A 3451185 unspecified role unspecified role bait bait purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) association association false false false Fig 1C a5k736_plavs-tfrc-1 3451180 N/A 3451184 unspecified role unspecified role prey prey purified purified spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. N/A 3451185 unspecified role unspecified role bait bait purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 1C a5k736_plavs-tfrc-2 3451181 N/A 3451184 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false DyLight594 DyLight594 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. N/A 3451185 unspecified role unspecified role fluorescence donor fluorescence donor purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false DyLight488 DyLight488 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig S2-B a5k736_plavs-tfrc-3 3451182 N/A 3451184 unspecified role unspecified role prey prey purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. N/A 3451185 unspecified role unspecified role bait bait purified purified sufficient binding region sufficient binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 3A, Fig 3B