MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint pitre-2017-2 human-m07e Homo sapiens hematopoietic cells m07e human hematopoietic cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-1 in vitro In vitro competition binding competition binding western blot western blot Immuno blot Immuno blot Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-3 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-5 human-m07e Homo sapiens hematopoietic cells m07e human hematopoietic cells pla proximity ligation assay pELISA pELISA PLA anti tag immunost anti tag immunostaining anti tag immunost Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-6 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-8 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pla proximity ligation assay pELISA pELISA PLA anti tag immunost anti tag immunostaining anti tag immunost Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-9 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation pitre-2017-10 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Pitre A., Ge Y., Lin W., Wang Y., Fukuda Y., Temirov J., Phillips AH., Peters JL., Fan Y., Ma J., Nourse A., Sinha C., Lin H., Kriwacki R., Downing JR., Gruber TA., Centonze VE., Naren AP., Chen T., Schuetz JD. john.schuetz@stjude.org Nature communications 2017 Only protein-protein interactions imex curation imex curation mrp4_human ATP-binding cassette sub-family C member 4 ABCC4 MRP4 MRP/cMOAT-related ABC transporter Multi-specific organic anion transporter B MOATB Multidrug resistance-associated protein 4 protein protein human Homo sapiens Human MLPVYQEVKPNPLQDANLCSRVFFWWLNPLFKIGHKRRLEEDDMYSVLPEDRSQHLGEELQGFWDKEVLRAENDAQKPSLTRAIIKCYWKSYLVLGIFTLIEESAKVIQPIFLGKIINYFENYDPMDSVALNTAYAYATVLTFCTLILAILHHLYFYHVQCAGMRLRVAMCHMIYRKALRLSNMAMGKTTTGQIVNLLSNDVNKFDQVTVFLHFLWAGPLQAIAVTALLWMEIGISCLAGMAVLIILLPLQSCFGKLFSSLRSKTATFTDARIRTMNEVITGIRIIKMYAWEKSFSNLITNLRKKEISKILRSSCLRGMNLASFFSASKIIVFVTFTTYVLLGSVITASRVFVAVTLYGAVRLTVTLFFPSAIERVSEAIVSIRRIQTFLLLDEISQRNRQLPSDGKKMVHVQDFTAFWDKASETPTLQGLSFTVRPGELLAVVGPVGAGKSSLLSAVLGELAPSHGLVSVHGRIAYVSQQPWVFSGTLRSNILFGKKYEKERYEKVIKACALKKDLQLLEDGDLTVIGDRGTTLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVSRHLFELCICQILHEKITILVTHQLQYLKAASQILILKDGKMVQKGTYTEFLKSGIDFGSLLKKDNEESEQPPVPGTPTLRNRTFSESSVWSQQSSRPSLKDGALESQDTENVPVTLSEENRSEGKVGFQAYKNYFRAGAHWIVFIFLILLNTAAQVAYVLQDWWLSYWANKQSMLNVTVNGGGNVTEKLDLNWYLGIYSGLTVATVLFGIARSLLVFYVLVNSSQTLHNKMFESILKAPVLFFDRNPIGRILNRFSKDIGHLDDLLPLTFLDFIQTLLQVVGVVSVAVAVIPWIAIPLVPLGIIFIFLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQGLWTIRAYKAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAMFVIIVAFGSLILAKTLDAGQVGLALSYALTLMGMFQWCVRQSAEVENMMISVERVIEYTDLEKEAPWEYQKRPPPAWPHEGVIIFDNVNFMYSPGGPLVLKHLTALIKSQEKVGIVGRTGAGKSSLISALFRLSEPEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALQEVQLKETIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILRKNQILIIDEATANVDPRTDELIQKKIREKFAHCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYVLLQNKESLFYKMVQQLGKAEAAALTETAKQVYFKRNYPHIGHTDHMVTNTSNGQPSTLTIFETAL em55_human 55 kDa erythrocyte membrane protein Membrane protein, palmitoylated 1 MPP1 EMP55 DXS552E protein protein human Homo sapiens Human MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY mpp1-abcc4-1 3473022 N/A 3473031 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3473032 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f2b abcc4-1 3473022 N/A 3473031 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3473032 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f2b abcc4-mpp1-1 3473023 N/A 3473031 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3473032 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false f1f abcc4-2 3473024 N/A 3473031 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3473032 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false f2c abcc4-mpp1-2 3473025 N/A 3473031 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3473032 unspecified role unspecified role bait bait purified purified gst tag gpires glycosylphosphatidylinositolated residue LIPID GPI-anchor amidated carboxyl end GPIRes GPIanchor glycosylphosphatidylinositol undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding range required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false f2f abcc4-mpp1-3 3473026 N/A 3473031 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell N/A 3473032 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell proximity proximity close-contact colocalization neighbourhood interaction false false false f2g abcc4-mpp1-4 3473027 N/A 3473031 unspecified role unspecified role neutral component neutral component purified purified binding range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false fluorescin tag fluorescein fluorescein label fluorescein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3473032 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3473027 sf1c abcc4-mpp1-5 3473028 N/A 3473031 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell binding range required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3473032 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell proximity proximity close-contact colocalization neighbourhood interaction false false false sf2 abcc4-mpp1-6 3473029 N/A 3473031 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false binding range required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3473032 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell physical association physical association false false false sf3b sf3c abcc4-mpp1-7 3473030 N/A 3473031 unspecified role unspecified role neutral component neutral component purified purified binding range required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false biontin tag biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3473032 unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3473030 f5a f5b