DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ bononi-2017-4 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. MCarbone@cc.hawaii.edu Nature 2017 imex curation Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M., Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. Only protein-protein interactions imex curation bononi-2017-7 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. MCarbone@cc.hawaii.edu Nature 2017 imex curation Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M., Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. Only protein-protein interactions imex curation bononi-2017-1 human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. MCarbone@cc.hawaii.edu Nature 2017 imex curation Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M., Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. Only protein-protein interactions imex curation bononi-2017-2 human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. MCarbone@cc.hawaii.edu Nature 2017 imex curation Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M., Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. Only protein-protein interactions imex curation bononi-2017-3 human-fibroblast Homo sapiens normal diploid fibroblasts (HDF0 fibroblast Fibroblast Resident cell of connective tissue, mesodermally derived confocal microscopy confocal microscopy immunostaining immunostaining Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. MCarbone@cc.hawaii.edu Nature 2017 imex curation Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M., Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. Only protein-protein interactions imex curation bononi-2017-5 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. MCarbone@cc.hawaii.edu Nature 2017 imex curation Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M., Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. Only protein-protein interactions imex curation bononi-2017-6 in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay predetermined predetermined participant predetermined Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. MCarbone@cc.hawaii.edu Nature 2017 imex curation Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M., Bononi A., Giorgi C., Patergnani S., Larson D., Verbruggen K., Tanji M., Pellegrini L., Signorato V., Olivetto F., Pastorino S., Nasu M., Napolitano A., Gaudino G., Morris P., Sakamoto G., Ferris LK., Danese A., Raimondi A., Tacchetti C., Kuchay S., Pass HI., Affar EB., Yang H., Pinton P., Carbone M. Only protein-protein interactions imex curation bap1_human Ubiquitin carboxyl-terminal hydrolase BAP1 BAP1 KIAA0272 hucep-6 BRCA1-associated protein 1 Cerebral protein 6 protein protein human Homo sapiens Human MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGSCAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYKGKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ itpr3_human Inositol 1,4,5-trisphosphate receptor type 3 ITPR3 Type 3 inositol 1,4,5-trisphosphate receptor IP3 receptor isoform 3 protein protein human Homo sapiens Human MSEMSSFLHIGDIVSLYAEGSVNGFISTLGLVDDRCVVEPAAGDLDNPPKKFRDCLFKVCPMNRYSAQKQYWKAKQTKQDKEKIADVVLLQKLQHAAQMEQKQNDTENKKVHGDVVKYGSVIQLLHMKSNKYLTVNKRLPALLEKNAMRVTLDATGNEGSWLFIQPFWKLRSNGDNVVVGDKVILNPVNAGQPLHASNYELSDNAGCKEVNSVNCNTSWKINLFMQFRDHLEEVLKGGDVVRLFHAEQEKFLTCDEYKGKLQVFLRTTLRQSATSATSSNALWEVEVVHHDPCRGGAGHWNGLYRFKHLATGNYLAAEENPSYKGDASDPKAAGMGAQGRTGRRNAGEKIKYCLVAVPHGNDIASLFELDPTTLQKTDSFVPRNSYVRLRHLCTNTWIQSTNVPIDIEEERPIRLMLGTCPTKEDKEAFAIVSVPVSEIRDLDFANDASSMLASAVEKLNEGFISQNDRRFVIQLLEDLVFFVSDVPNNGQNVLDIMVTKPNRERQKLMREQNILKQVFGILKAPFREKGGEGPLVRLEELSDQKNAPYQHMFRLCYRVLRHSQEDYRKNQEHIAKQFGMMQSQIGYDILAEDTITALLHNNRKLLEKHITKTEVETFVSLVRKNREPRFLDYLSDLCVSNHIAIPVTQELICKCVLDPKNSDILIRTELRPVKEMAQSHEYLSIEYSEEEVWLTWTDKNNEHHEKSVRQLAQEARAGNAHDENVLSYYRYQLKLFARMCLDRQYLAIDEISQQLGVDLIFLCMADEMLPFDLRASFCHLMLHVHVDRDPQELVTPVKFARLWTEIPTAITIKDYDSNLNASRDDKKNKFANTMEFVEDYLNNVVSEAVPFANEEKNKLTFEVVSLAHNLIYFGFYSFSELLRLTRTLLGIIDCVQGPPAMLQAYEDPGGKNVRRSIQGVGHMMSTMVLSRKQSVFSAPSLSAGASAAEPLDRSKFEENEDIVVMETKLKILEILQFILNVRLDYRISYLLSVFKKEFVEVFPMQDSGADGTAPAFDSTTANMNLDRIGEQAEAMFGVGKTSSMLEVDDEGGRMFLRVLIHLTMHDYAPLVSGALQLLFKHFSQRQEAMHTFKQVQLLISAQDVENYKVIKSELDRLRTMVEKSELWVDKKGSGKGEEVEAGAAKDKKERPTDEEGFLHPPGEKSSENYQIVKGILERLNKMCGVGEQMRKKQQRLLKNMDAHKVMLDLLQIPYDKGDAKMMEILRYTHQFLQKFCAGNPGNQALLHKHLHLFLTPGLLEAETMQHIFLNNYQLCSEISEPVLQHFVHLLATHGRHVQYLDFLHTVIKAEGKYVKKCQDMIMTELTNAGDDVVVFYNDKASLAHLLDMMKAARDGVEDHSPLMYHISLVDLLAACAEGKNVYTEIKCTSLLPLEDVVSVVTHEDCITEVKMAYVNFVNHCYVDTEVEMKEIYTSNHIWTLFENFTLDMARVCSKREKRVADPTLEKYVLSVVLDTINAFFSSPFSENSTSLQTHQTIVVQLLQSTTRLLECPWLQQQHKGSVEACIRTLAMVAKGRAILLPMDLDAHISSMLSSGASCAAAAQRNASSYKATTRAFPRVTPTANQWDYKNIIEKLQDIITALEERLKPLVQAELSVLVDVLHWPELLFLEGSEAYQRCESGGFLSKLIQHTKDLMESEEKLCIKVLRTLQQMLLKKTKYGDRGNQLRKMLLQNYLQNRKSTSRGDLPDPIGTGLDPDWSAIAATQCRLDKEGATKLVCDLITSTKNEKIFQESIGLAIHLLDGGNTEIQKSFHNLMMSDKKSERFFKVLHDRMKRAQQETKSTVAVNMNDLGSQPHEDREPVDPTTKGRVASFSIPGSSSRYSLGPSLRRGHEVSERVQSSEMGTSVLIMQPILRFLQLLCENHNRDLQNFLRCQNNKTNYNLVCETLQFLDIMCGSTTGGLGLLGLYINEDNVGLVIQTLETLTEYCQGPCHENQTCIVTHESNGIDIITALILNDISPLCKYRMDLVLQLKDNASKLLLALMESRHDSENAERILISLRPQELVDVIKKAYLQEEERENSEVSPREVGHNIYILALQLSRHNKQLQHLLKPVKRIQEEEAEGISSMLSLNNKQLSQMLKSSAPAQEEEEDPLAYYENHTSQIEIVRQDRSMEQIVFPVPGICQFLTEETKHRLFTTTEQDEQGSKVSDFFDQSSFLHNEMEWQRKLRSMPLIYWFSRRMTLWGSISFNLAVFINIIIAFFYPYMEGASTGVLDSPLISLLFWILICFSIAALFTKRYSIRPLIVALILRSIYYLGIGPTLNILGALNLTNKIVFVVSFVGNRGTFIRGYKAMVMDMEFLYHVGYILTSVLGLFAHELFYSILLFDLIYREETLFNVIKSVTRNGRSILLTALLALILVYLFSIVGFLFLKDDFILEVDRLPNNHSTASPLGMPHGAAAFVDTCSGDKMDCVSGLSVPEVLEEDRELDSTERACDTLLMCIVTVMNHGLRNGGGVGDILRKPSKDESLFPARVVYDLLFFFIVIIIVLNLIFGVIIDTFADLRSEKQKKEEILKTTCFICGLERDKFDNKTVSFEEHIKLEHNMWNYLYFIVLVRVKNKTDYTGPESYVAQMIKNKNLDWFPRMRAMSLVSNEGEGEQNEIRILQDKLNSTMKLVSHLTAQLNELKEQMTEQRKRRQRLGFVDVQNCISR itpr3-bap1-4 3472386 N/A 3472393 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3472394 unspecified role unspecified role bait bait native native physical association physical association false false false Extended Data Fig 6b itpr3-bap1-5 3472386 N/A 3472393 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false required to bind required to bind required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 3472394 unspecified role unspecified role bait bait native native physical association physical association false false false Fig 2F itpr3-bap1-6 3472386 N/A 3472393 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q92560:p.Cys91Ser C91S mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 3472394 unspecified role unspecified role bait bait native native physical association physical association false false false Extended Data Fig 6c itpr3-bap1-7 3472387 N/A 3472393 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3472394 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait native native sufficient to bind HA-IP3R3(NT) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 2G itpr3-bap1-8 3472387 N/A 3472393 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed sufficient to bind BAP1(NORS) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3472394 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey native native sufficient to bind HA-IP3R3(NT) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Extended Data Fig 6e,f itpr3-bap1-9 3472387 N/A 3472393 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3472394 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3d itpr3-bap1-1 3472388 N/A 3472393 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level native native N/A 3472394 unspecified role unspecified role bait bait native native physical association physical association false false false Fig 2C itpr3-bap1-3 3472388 N/A 3472393 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 3472394 unspecified role unspecified role bait bait native native physical association physical association false false false Fig 2E itpr3-bap1-2 3472389 N/A 3472393 unspecified role unspecified role neutral component neutral component native native N/A 3472394 unspecified role unspecified role neutral component neutral component native native proximity proximity close-contact colocalization neighbourhood interaction false false false Fig 2D bap1-itpr3-1 3472390 N/A 3472393 unspecified role unspecified role neutral component neutral component native native N/A 3472394 unspecified role unspecified role neutral component neutral component native native colocalization colocalization false false false Extended Data Fig 6a bap1-itpr3-2 3472391 N/A 3472393 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3472394 unspecified role unspecified role prey prey native native physical association physical association false false false Extended Data Fig 6b bap1-itpr3-3 3472392 N/A 3472393 anti tag western anti tag western blot anti tag western enzyme enzyme unspecified role unspecified role over-expressed over expressed level over-expressed Myc tag Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q92560:p.Cys91Ser C91S mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 3472394 anti tag western anti tag western blot anti tag western enzyme target enzyme target substrate substrate unspecified role unspecified role over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Ubquitin Ubquitin ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. deubiquitination deubiquitination reaction deubiquitination false false false Fig 3e