UniProt UniProt http://www.uniprot.org wang-2017-22 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP peptide confirmed by author liuqiong@szu.edu.cn Biochemical and biophysical research communications 2017 Only protein-protein interactions imex curation imex curation wang-2017-21 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. frap fluorescence recovery after photobleaching frap tag fluorescence tag visualisation by fluorescence tag fluorescence Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease A FRET signal was measured following photo-bleaching. The change in donor fluorescence induced by acceptor photo-bleaching was quantified by comparing pre-bleach and post-bleach images giving a measure both of FRET efficiency and the distance between the interactive proteins. Source of APP peptide confirmed by author liuqiong@szu.edu.cn Biochemical and biophysical research communications 2017 Only protein-protein interactions imex curation imex curation wang-2017-23 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Wang C., Chen P., He X., Peng Z., Chen S., Zhang R., Cheng J., Liu Q. Alzheimers - Interactions investigated in the context of Alzheimers disease Source of APP peptide confirmed by author liuqiong@szu.edu.cn Biochemical and biophysical research communications 2017 Only protein-protein interactions imex curation imex curation msrb1_human Methionine-R-sulfoxide reductase B1 MSRB1 SEPX1 Selenoprotein X HSPC270 protein protein human Homo sapiens Human MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRSEALKVSCGKCGNGLGHEFLNDGPKPGQSRFUIFSSSLKFVPKGKETSASQGH p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 app-msrb1-2 3519347 N/A 3519350 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3519351 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 2 Interaction confirmed by anti-bait western msrb1-app-1 3519347 N/A 3519350 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3519351 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 2 Interaction confirmed by anti-bait western app-msrb1-1 3519348 N/A 3519350 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3519351 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 1 msrb1-app-2 3519349 N/A 3519350 unspecified role unspecified role bait bait purified purified region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3519351 unspecified role unspecified role prey prey purified purified region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction false false false Fig 3 Interaction confirmed by anti-bait western