MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint lin-2012-18 human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lin YS., Chen YJ., Cohen SN., Cheng TH. thcheng@ym.edu.tw PloS one 2012 imex curation Only protein-protein interactions imex curation cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human ts101_human Tumor susceptibility gene 101 protein TSG101 ESCRT-I complex subunit TSG101 protein protein human Homo sapiens Human MAVSESQLKKMVSKYKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVVFGDEPPVFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGGPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY tsg101-cdkn1a_human_gene-1 3450989 N/A 3450990 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level promoter region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false CAGCCTGCTCCCTTGCCTTTTTCA GACCCCACTCTAAGCCCCACTGC N/A 3450991 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level binding region binding region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false f3a f4b Chromatin immunoprecipitation (ChIP) assays showed that sequences of the p21 promoter region interact directly with the TSG101 protein and identified the TSG101-binding sequence in Saos-2 as the region amplified by primer set “d” (Fig 3A). However, failure of a construct containing only the UEV domain to bind indicates that at the presence of either the PR or CC domain enables binding. Collectively these findings indicate that TSG101 contains more than one locus that allows it to interact with the p21 promoter. Q99816 activates ENSG00000124762 down-regulates activity