IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ takyar-2013-1 mouse-mle12 Mus musculus mle12 MLE12 lung epithelial cell mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Takyar S., Vasavada H., Zhang JG., Ahangari F., Niu N., Liu Q., Lee CG., Cohn L., Elias JA. seyedtaghi.takyar@yale.edu The Journal of experimental medicine 2013 Only protein-protein interactions imex curation imex curation takyar-2013-2 mouse-mlecs Mus musculus mlecs mlecs rna-ip rna immunoprecipitation rna-ip qrt-pcr quantitative reverse transcription pcr QRT-PCR Takyar S., Vasavada H., Zhang JG., Ahangari F., Niu N., Liu Q., Lee CG., Cohn L., Elias JA. seyedtaghi.takyar@yale.edu The Journal of experimental medicine 2013 Only protein-protein interactions imex curation imex curation mrna_mpl1 myeloproliferative leukemia virus oncogene mrna messenger rna mRNA mrna mouse Mus musculus Mouse GAAGGGAGGATGGGCTAAGGCAGGCACACAGTGCCGGAGAAGATGCCCTCTTGGGCCCTCTTCATGGTCACCTCCTGCCTCCTCTTGGCCCTTCCAAACCAGGCACAAGTCACCAGCCAAGATGTCTTCTTGCTGGCCTTGGGCACAGAGCCCCTGAACTGCTTCTCCCAAACATTTGAGGACCTCACCTGCTTCTGGGATGAGGAAGAGGCAGCACCCAGTGGGACATACCAGCTGCTGTATGCCTACCGAGGAGAGAAGCCCCGTGCATGCCCCCTGTATTCCCAGAGTGTGCCCACCTTTGGAACCCGGTATGTGTGCCAGTTTCCAGCCCAGGATGAAGTGCGCCTCTTCTTTCCGCTGCACCTCTGGGTGAAGAATGTGTCCCTCAACCAGACTTTGATCCAGCGGGTGCTGTTTGTGGATAGTGTGGGCCTGCCAGCTCCCCCCAGGGTCATCAAGGCCAGGGGTGGGAGCCAACCAGGGGAACTTCAGATCCACTGGGAGGCCCCTGCTCCTGAAATCAGTGACTTCCTGAGGCATGAACTCCGCTATGGCCCCACGGACTCCAGCAACGCCACTGCCCCCTCCGTCATTCAGCTGCTCTCCACAGAAACCTGCTGCCCCACTTTGTGGATGCCGAACCCAGTCCCTGTTCTTGACCAGCCTCCGTGTGTTCATCCGACAGCATCCCAACCGCATGGACCAGCTCCATTTCTGACAGTGAAGGGTGGAAGCTGTCTCGTCTCAGGCCTCCAGGCTGGCAAATCCTACTGGCTCCAGCTACGCAGCCAACCCGACGGGGTCTCCCTTCGTGGCTCCTGGGGACCCTGGTCCTTCCCTGTGACTGTGGATCTTCCAGGAGATGCAGTGACAATTGGACTTCAGTGCTTTACCTTGGATCTGAAGATGGTCACCTGCCAGTGGCAGCAACAAGACCGCACTAGCTCCCAAGGCTTCTTCCGCCACAGCAGGACGAGGTGCTGCCCCACAGACAGGGACCCCACCTGGGAGAAATGTGAAGAGGAGGAACCGCGTCCAGGATCACAGCCCGCTCTCGTCTCCCGCTGCCACTTCAAGTCACGAAATGACAGTGTTATTCACATCCTTGTAGAGGTGACCACAGCGCAAGGTGCCGTTCACAGCTACCTGGGCTCCCCTTTTTGGATCCACCAGGCTGTGCTCCTTCCCACCCCGAGCCTGCACTGGAGGGAGGTCTCAAGTGGAAGGCTGGAGTTGGAGTGGCAGCACCAGTCATCTTGGGCAGCTCAAGAGACCTGCTACCAGCTCCGGTACACGGGAGAAGGCCGTGAGGACTGGAAGGTGCTGGAGCCATCTCTCGGTGCCCGGGGAGGGACCCTAGAGCTGCGCCCCCGAGCTCGCTACAGCTTGCAGCTGCGTGCCAGGCTCAACGGCCCCACCTACCAAGGTCCCTGGAGCGCCTGGTCTCCCCCAGCTAGGGTGTCCACGGGCTCCGAGACTGCTTGGATCACCTTGGTGACTGCTCTGCTCCTGGTGCTGAGCCTCAGTGCCCTTCTGGGCCTACTGCTGCTAAAGTGGCAATTTCCTGCGCACTACAGGAGACTGAGGCATGCTTTGTGGCCCTCGCTTCCAGACCTACACCGGGTCCTAGGCCAGTACCTCAGAGACACTGCAGCCCTAAGTCCTTCTAAGGCCACGGTTACCGATAGCTGTGAAGAAGTGGAACCCAGCCTCCTGGAAATCCTCCCTAAGTCCTCAGAGAGCACTCCTTTACCTCTGTGTCCCTCCCAACCTCAGATGGACTACAGAGGACTGCAACCTTGCCTGCGGACCATGCCCCTGTCTGTGTGTCCACCCATGGCTGAGACGGGGTCCTGCTGCACCACACACATTGCCAACCACTCCTACCTACCACTAAGCTATTGGCAGCAGCCCTGAAGGCAGTCCCCATGCTACTGCAGACCTATACATTCCTACACACTACCTTATCCATCCTCAACACCATCCATTCTGTTGCCACCCCACTCCCCCTCTGGCTTTATAACACTGATCACTCCAGATGGCTGCTCACAAATCCAGAGCTCTGTCTCTGCAGCTGCTTGGCTCCTCTGGCTGTTTATTTTTATTTTTTTAATTTTTTAGACACTGTTTATTATATAACTCTGATGACCTATAACTTGCTATGTAGAAACGGCTGGCCTCAAACTCACAGAAATCTGCCTGCTGTGACTAAAGTTGCCATGCCCTGTAGCTCCAAACCCTTGGGGGTGGGGAAAGTCTCTGTTTCCCTATCTCCTACAGGCTTTAAAGAGTTCTTCAGTGAGTCCCCTAGCTTGCTGTTAAGGGAACTGTGCAAGCCTTCTAATTATGAGACCCAGTGGGGTCTTGCTGTCATCTATTTATTTGATCTCTCTTGGCATCCTCCTCCACCCTGGGTGCCACAGTATCACCTTCCTTCCTGGTTCTGTTCCTGCCTCTCTGAGCATACTAGCAGCAAAAGAGCCAACTCTGGAGCTAGAACCAGGTTCTGGTCCTGTCGTTCACACCTGCTAGCTGTGCAAACTTAGGCAAATAGCCTTTGAGCCTTTTCCCTCTCATCTACGAAGTGTGGATAATAACAGAACCCCATAGAGCAGTGTGAACAATTGAGAACACACTGGCTGGGACAGTCAATGCTTAAAATGCCCCAATTAATGTCCTAAGTATTTCTTCTCTTCCTATTCTGGCCTTTGTTACATGATGACCATCAGAAAGTTCTTATCTCCAGCCCAGACTTCTATAGATAGATCCCACAGTGTTTTCTGTGGATTTTAACATTTGTTTCTTGCTCCTGGATAGTGAGTTGAAAGGCAAGGGTTAGGATCTGTCCTTACCCCCCACCCCCACCCCAGCATCTTCAATACTATTTCACGTATGATAGGTGGCAGACCTCCAATAAATATCGTCTGAATG mmu-mir-1a 3p miR-1a 3p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA mouse Mus musculus Mouse UGGAAUGUAAAGAAGUAUGUAU ago2_mouse Protein argonaute-2 Ago2 Argonaute RISC catalytic component 2 Eif2c2 Eukaryotic translation initiation factor 2C 2 Kiaa4215 Piwi/argonaute family protein meIF2C2 Protein slicer protein protein mouse Mus musculus Mouse MYSGAGPVLASPAPTTSPIPGYAFKPPPRPDFGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA mmu_mir_1a 3p-1 3465506 N/A 3465508 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false r.1942_1956delinstgacatacactctca mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3465509 predetermined predetermined participant predetermined unspecified role unspecified role unspecified role unspecified role engineered engineered purified purified physical association physical association false false false Fg 5H down-regulates quantity by destabilization mmu_mir_1a 3p-mrna_mpl1-1 3465507 N/A 3465510 antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3465508 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3465509 predetermined predetermined participant predetermined unspecified role unspecified role prey prey engineered engineered purified purified association association false false false Fg 5B,C,D