IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ georges-2010-1 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Georges A., Benayoun BA., Marongiu M., Dipietromaria A., L'Hôte D., Todeschini AL., Auer J., Crisponi L., Veitia RA. we have performed a FOXL2 two-hybrid screen using a mouse ovary cDNA library which retrieved a potential interaction between FOXL2 and the E3 SUMO-protein ligase PIAS2 (2 prey clones), the SUMO-conjugating enzyme UBC9 (2 prey clones) and the nuclear autoantigen SP100, which is an organizer of PML-NBs (2 clones). FOXL2 was inferred to be human by blasting peptides provided in Table 1 and 2. veitia.reiner@ijm.univ-paris-diderot.fr mouse ovary cDNA library PloS one 2010 imex curation Only protein-protein interactions imex curation pias2_mouse E3 SUMO-protein ligase PIAS2 Pias2 Miz1 Piasx Androgen receptor-interacting protein 3 DAB2-interacting protein Msx-interacting zinc finger protein Protein inhibitor of activated STAT x Protein inhibitor of activated STAT2 RING-type E3 ubiquitin transferase PIAS2 protein protein mouse Mus musculus Mouse MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLCDLSTIKSSVFSLDGSSSPVEPDLPVAGIHSLPSTSITPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRPKKEAMKVTSQPCTKVESSSVFSKPCSVTVASDASKKKIDVIDLTIESSSDEEEDPPAKRKCIFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPVSSMSSDLPGLDFLSLIPVDPQYCPPMFLDSLTSPLTASSTSVTTTSPHESSTHVSSSSSRSETGVITSSGRNIPDIISLD foxl2_human Forkhead box protein L2 FOXL2 protein protein human Homo sapiens Human MMASYPEPEDAAGALLAPETGRTVKEPEGPPPSPGKGGGGGGGTAPEKPDPAQKPPYSYVALIAMAIRESAEKRLTLSGIYQYIIAKFPFYEKNKKGWQNSIRHNLSLNECFIKVPREGGGERKGNYWTLDPACEDMFEKGNYRRRRRMKRPFRPPPAHFQPGKGLFGAGGAAGGCGVAGAGADGYGYLAPPKYLQSGFLNNSWPLPQPPSPMPYASCQMAAAAAAAAAAAAAAGPGSPGAAAVVKGLAGPAASYGPYTRVQSMALPPGVVNSYNGLGGPPAAPPPPPHPHPHPHAHHLHAAAAPPPAPPHHGAAAPPPGQLSPASPATAAPPAPAPTSAPGLQFACARQPELAMMHCSYWDHDSKTGALHSRLDL ubc9_mouse SUMO-conjugating enzyme UBC9 Ube2i Ubc9 Ubce9 Ubce2i SUMO-protein ligase Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I Ubiquitin carrier protein I Ubiquitin carrier protein 9 RING-type E3 SUMO transferase UBC9 protein protein mouse Mus musculus Mouse MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS sp100_mouse Nuclear autoantigen Sp-100 Nuclear dot-associated Sp100 protein Speckled 100 kDa Sp100 protein protein mouse Mus musculus Mouse MEGSDGSPRMSTEQENTEMHLIECMLKHFKTQKVAISNAIRSTFPFLESLRDHEFITGKMYEDLLDSCRSLVPVDKVIYRALEELEKKFDMTVLCELFNEVNMEKYPDLNLIRRSFGCVFPNELCFQGIDGGNPNSQLSLEQGPGASYSQGSPNGSSLDLSASEGWRSNDRRNSNLMQANQTENHQLAESPGHLDSCELQVQLNNRDATPESCSLLPQNEERAVQLNYELQINPCFVQLVDVKKENSSFSLAGNQQTRARTNQNEDSEIIELSSGDSDNGENFSEATTTVPSQPAPAYSRKPPTLRRDRGGDTSDTESSIIIRRRKRTGRKKRERLGSYLIRNIKIPMKPSWKTAFLARSANPSSQRRRKRGPRIPREENADFGGAELPVVCGNAQGFLDKEKFKQGIYVRSIRGKTGRLFTPMDFEIEGNCEKAKNWRQSIRCKGWTLRELIQKGVLQDPPRKKKETPRNPRQTRRQVNAL foxl2-pias2-1 3474902 N/A 3474903 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3474904 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association false false false na foxl2-ube2i-1 3474902 N/A 3474905 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3474904 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association false false false na foxl2-sp100-1 3474902 N/A 3474906 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3474904 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association false false false na