IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ qiao-2011-2 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Qiao Y., Ma N., Wang X., Hui Y., Li F., Xiang Y., Zhou J., Zou C., Jin J., Lv G., Jin H., Gao X., Qiao Y., Ma N., Wang X., Hui Y., Li F., Xiang Y., Zhou J., Zou C., Jin J., Lv G., Jin H., Gao X. kinga@ems.hrbmu.edu.cn FEBS letters 2011 imex curation Only protein-protein interactions imex curation mrna_srf mRNA serum response factor mrna messenger rna mRNA mrna human Homo sapiens Human GAAGGGTCGGGGGATCCCTCCGCCGCCAGCGCGTGGTCCCGGCCCCCTCCACCCGCCGTCTCGGCCGCGGCCAGCAGCCCCTGCCCCCCGGGGGACGCTGACGGCCGCCCGGCGCGCCGCCCTAGCAGACGGACAGGGGGCGCTGCGCGCGGCCTGGGGCAACCCGGGCCACAGGGGCAGGAAAGTGAGGGCCCAGGTCGGCCCGGGCGTGCAGGGGCCCCGGGTTCGCAGCGGCGGCCGCGGCAGCGATAGCGGCACTAGCAGCAGCGGGAGTGCCGGGTTGAGCCGGGAAGCCGATGGCGGCGGCTGCGGCGGCTCCGATTCCTCGCTGACTGCCCGTCCGCCCTCCTGCATCGAGCGCCATGTTACCGACCCAAGCTGGGGCCGCGGCGGCTCTGGGCCGGGGCTCGGCCCTGGGGGGCAGCCTGAACCGGACCCCGACGGGGCGGCCGGGCGGCGGCGGCGGGACACGCGGGGCTAACGGGGGCCGGGTCCCCGGGAATGGCGCGGGGCTCGGGCCCGGCCGCCTGGAGCGGGAGGCTGCGGCAGCGGCGGCAACCACCCCGGCGCCCACCGCGGGGGCCCTCTACAGCGGCAGCGAGGGCGACTCGGAGTCGGGCGAGGAGGAGGAGCTGGGCGCCGAGCGGCGCGGCCTGAAGCGGAGCCTGAGCGAGATGGAGATCGGTATGGTGGTCGGTGGGCCCGAGGCGTCGGCAGCGGCCACCGGGGGCTACGGGCCGGTGAGCGGCGCGGTGAGCGGGGCCAAGCCGGGTAAGAAGACCCGGGGCCGCGTGAAGATCAAGATGGAGTTCATCGACAACAAGCTGCGGCGCTACACGACCTTCAGCAAGAGGAAGACGGGCATCATGAAGAAGGCCTATGAGCTGTCCACGCTGACAGGGACACAGGTGCTGTTGCTGGTGGCCAGTGAGACAGGCCATGTGTATACCTTTGCCACCCGAAAACTGCAGCCCATGATCACCAGTGAGACCGGCAAGGCACTGATTCAGACCTGCCTCAACTCGCCAGACTCTCCACCCCGTTCAGACCCCACAACAGACCAGAGAATGAGTGCCACTGGCTTTGAAGAGACAGATCTCACCTACCAGGTGTCGGAGTCTGACAGCAGTGGGGAGACCAAGGACACACTGAAGCCGGCGTTCACAGTCACCAACCTGCCGGGTACAACCTCCACCATCCAAACAGCACCTAGCACCTCTACCACCATGCAAGTCAGCAGCGGCCCCTCCTTTCCCATCACCAACTACCTGGCACCAGTGTCTGCTAGTGTCAGCCCCAGTGCTGTCAGCAGTGCCAATGGGACTGTGCTGAAGAGTACAGGCAGCGGCCCTGTCTCCTCTGGGGGCCTTATGCAGCTGCCTACCAGCTTCACCCTCATGCCTGGTGGGGCAGTGGCCCAGCAGGTCCCAGTGCAGGCCATTCAAGTGCACCAGGCCCCACAGCAAGCGTCTCCCTCCCGTGACAGCAGCACAGACCTCACGCAGACCTCCTCCAGCGGGACAGTGACGCTGCCCGCCACCATCATGACGTCATCCGTGCCCACAACTGTGGGTGGCCACATGATGTACCCTAGCCCGCATGCGGTGATGTATGCCCCCACCTCGGGCCTGGGTGATGGCAGCCTCACCGTGCTGAATGCCTTCTCCCAGGCACCATCCACCATGCAGGTGTCACACAGCCAGGTCCAGGAGCCAGGTGGCGTCCCCCAGGTGTTCCTGACAGCATCATCTGGGACAGTGCAGATCCCTGTTTCAGCAGTTCAGCTCCACCAGATGGCTGTGATAGGGCAGCAGGCCGGGAGCAGCAGCAACCTCACCGAGCTACAGGTGGTGAACCTGGACACCGCCCACAGCACCAAGAGTGAATGATCCGCCCGCCGCCCTGGACAGATGGCCCAAGGGATGGCACCACTTATTTATTGTTGCCTTTTCACGTTTTCTTTACACACACGTTGACGGGCCGCAGGAGGGAGGCGGGGAGGAGGAACGGGCAGCCACAGGACTGAGCCCTCTCACTCCAGCCAAAGAAATGGGCCTGCCTGCCTCCACCCGTCCTCCCTCAGCCTCCCCTTCTTCCCGCCCCACCTCCCATTTCTGTTGCTGGAGGGGCTGTCCTCCTTCCTGGGACCCCCTCGCCAGCTTGGCTCGATGTTTGCCATGAGTATTAGCTTACCCAATGGGACCGTGCCCCACCTCCCCACACACAGGCCTTCTGTGGGGCTGGGCACCGTGTCCTCCTCTGAGGAAGCAGTTGGGGCCCTCTTGCCAGCCTCCTTGCTGACCCCAGGTCAGCCCTGTGTCTGTCACAGGCTGGGTCAAAAGAGCCCTGGCTCTGCCCCTCAGGGGGCCAGCTGGGGAGATGGGGGCTTCTTCCTCACACTGCTGTCCTCTCCCCCTTCAGCTCCTGAGTAGCTGGGCCTGTGCACTGGGCAGGTTCCTGGGGCCGCCTGCCCTGCCTTGCCGCTCCCCTTGGACCTCCAGGGGCTCCTGGGTTGGAGGGAACCACCAGCGTTCCCTTCTCCCCCTTGTCTTCCCCCCTCTCCTCCCAGCTGCTTTACTTAAAGTTGATTTTGAACTTTTTATTTGAGGAGACGAAGTGAAAACAAATCTATAAATATATATTTTTAAAATATTTAACTTTTTTTTATGGCGTTTTTCTCGTCCCCCTCCCTGCCCAAACTCCCCTTCCCTGGGGAGCCCTCAGGCTCCCCAGAACTGGCTGGGCCCCTGGGGACAGAGCCACCCCATGAGCTCGGGGTCCACCAGTGTGTGGGGGAGATTCTGGGTTTGCCCAGTCCTGGGTTGTTTCCAGGAGAAAGCCGGGGGAGGGGCCCTCAGGCCATTCCCCAACGGGGTGGGGAGGGTGACCCACAGCTCTGGGCCTCTTTTTGCCCTTTAGGGCTGTTGCTAGGGAGAGGGAAGAGGGAGACCAAATGTCGGGGTTGGGGTGGGAGGGCGTCAGGCAGAGGCAACTGACTTCATTTGTGCCACACGCATGGGCATTGCAGCCTTGCGCTGTCCCAGGCATGCAGCTGCCTGGGGCCCAAGTTGCAGTGAGCAGGGTGGGGTCTGGGAGGGGGTGAGAGGCAGGAATGGGGGTCAGAAGAAGTGGGAGCAGCTTCTTGGGCTGAGTGCAGCCAAAGGGGAGCCAGAAATGGGCAGTTCTCCCAGGGAGTGAGCAGCTACTGTAACTTTTTTAAATTAAGACAAAAAGCCTTGAAGAAAATGACTTTATTTTTCTAAGTGTAACCTCAGTATTTATGTAATTTGTACAGGGGCCATGCCCCACCCCCCTCCTCCCCCTTTGGGGTAGACCTTGAGGGTGGGCCAGCATAGGGGGGAGGGTCTTTTACCCTGTGTCAGAGCCTACCTTCACCACCTATATCCAGAAGGGGAGCTTTTTCAGAAACAGGGCAGCAGTGGGGTGAAATTTTCTTAACCCCTAAGACTGCCTTCAGTAGGAACAAGCTGGCTTCTGTGATTAGGTGAAGGGATGGGGGAAGATTTTATGCACAGCCTAGTTATCAAGGGGATGATTTGCCGACATGTTTGAGAACCCCCTAACCTCTAACCCTCATTGCTGTCTTGCCCCAGTTTGGGGTGCCAAGATGGAAGTCACCTTTCTGGGCTTTCTCCTGGAGATAGCTGGGGCTTATGGGTGGCTTTCAAGGCTGGGGCATGGCAAATCAGGGGCCAGAGAGCAGGGGAGCTTGGGACTCAGGTCTGTAACTGCCCAGCCCCTTTTCTCTGCTCTTGTTTCACTCCACCATCACTCACTCACTCCCCACTCCCCCACCCATGGGGAGGAGACCTTTGATGAATTCTTCCTCTCCTTCCCACAAAAGACAGACCCAGTGAGTGAATCAGGCAAAGTGCTTATAATGTGTGTTGTGTGAGCGTGGCCTTGGGAGGACATGCGTGTGTCAGGGATGAGTTGAGGTGATATTTTTATGTGCAGCGACCCTTGGTGTTTCCCTTCCTCGGTGGCTCTGGGGTATGTGTGTGTGGGTGTGTGCGCCTGAGTGAGTGTGTGTGCTTGAATGTGAGTGTGTATGTCAGTGGTTTCTACTTCCCCTGGGATGCTGACCCAGGAATAGTGGACATGGTCACAGTCCTATGTACAGAGCTTTCTTTTGTATTAAAAAAAAATACTCTTTCAATAAATGTATCATTTTTGTGCACAGAAAAAAAAAAAAAAAAAAAAAAAAAA hsa-mir-483-5p human miR-483-5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human AAGACGGGAGGAAAGAAGGGAG mir_483-mrna_srf-2 3340569 N/A 3340570 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered Tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false r.2555_2561delinsnnnnnnn mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false This construct contained only a fragment of 395bp coding for of the 3' end of the mRNA including the region 2542-2562 complementary to the miR. N/A 3340571 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified synthetic construct synthetic construct physical association physical association false false false Fig4B