IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ingermann-2010-4 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. ingermann-2010-5 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. ingermann-2010-7 in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. IGFBP-3R Binding Assay:A 96-well plate was coated with anti-FLAG antibody. Cos7 cell lysates were added followed by biotinylated IGFBP-3 in the presence of 0–100 nm IGFBP-3. After incubation HRP-conjugated streptavidin, followed by TMB were added. Absorbance was measured at 450 nm. The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. ingermann-2010-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu Hs578T cDNA library The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. ingermann-2010-2 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. It is not clear if this is an In Vitro or In Vivo experiment; in the absence of any stated cell line used for transfection, the experiment has been assume to have been carried out "In Vitro". The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. ingermann-2010-3 human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. ingermann-2010-6 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. Participant detection was by "I125-IGF-I/-II ligand blotting". Results from the competition by IGF1 (figure 2F) have not been annotated as the origin of IGF1 (R+D) is not stated. The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. ingermann-2010-8 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingermann AR., Yang YF., Han J., Mikami A., Garza AE., Mohanraj L., Fan L., Idowu M., Ware JL., Kim HS., Lee DY., Oh Y. The authors have stated that Caspase-8, in as used in Figure 5E - In vitro TNT experiment, is of human origin. yoh@mcvh-vcu.edu The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation IGFBP-3 has been entered as being of Human origin;the MCF-7:IGFBP-3 stable line (Ref 19) is of Human origin as is recombinant (E.coli) Celtrix IGFBP-3 (supplied by D. Mascarenhas -see PMID:14576163). As far as can be determined all other references refer to the Human protein. tm219_human Insulin-like growth factor-binding protein 3 receptor TMEM219 Transmembrane protein 219 protein protein human Homo sapiens Human MGNCQAGHNLHLCLAHHPPLVCATLILLLLGLSGLGLGSFLLTHRTGLRSPDIPQDWVSFLRSFGQLTLCPRNGTVTGKWRGSHVVGLLTTLNFGDGPDRNKTRTFQATVLGSQMGLKGSSAGQLVLITARVTTERTAGTCLYFSAVPGILPSSQPPISCSEEGAGNATLSPRMGEECVSVWSHEGLVLTKLLTSEELALCGSRLLVLGSFLLLFCGLLCCVTAMCFHPRRESHWSRTRL ibp3_human Insulin-like growth factor-binding protein 3 IGFBP3 IBP3 protein protein human Homo sapiens Human MQRARPTLWAAALTLLVLLRGPPVARAGASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK caspase8_chlae_protein Caspase 8 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey casp8_human Caspase-8 CASP8 MCH5 ICE-like apoptotic protease 5 MORT1-associated ced-3 homolog FADD-homologous ICE/ced-3-like protease FADD-like ICE Apoptotic cysteine protease Apoptotic protease Mch-5 CAP4 protein protein human Homo sapiens Human MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD tm219_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey tmem219-igfbp3-1 3433963 N/A 3433971 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3433972 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 2 C/D tmem219-igfbp3-5 3433963 N/A 3433971 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3433973 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 5D LHS tmem219-caspase8_chlae_protein-1 3433963 N/A 3433971 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3433973 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 5D RHS tmem219-igfbp3-2 3433964 N/A 3433971 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level conditioned medium conditioned medium human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells N/A 3433972 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma physical association physical association false false false Figure 2 E tmem219-igfbp3-6 3433964 N/A 3433971 unspecified role unspecified role bait bait partially purified partially purified in vitro In vitro N/A 3433974 unspecified role unspecified role prey prey partially purified partially purified in vitro In vitro physical association physical association false false false Figure 5E 24qa6twaert-1 3433965 N/A 3433972 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3433971 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 direct interaction direct interaction false false false Figure 2G LHS tmem219-igfbp3-4 3433965 N/A 3433972 competitor competitor competitor prey prey purified purified ecoli Escherichia coli (strain K12) N/A 3433972 unspecified role unspecified role prey prey purified purified region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3433971 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false 3433965 Figure 2G RHS 567r7u6dty-1 3433966 N/A 3433971 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell N/A 3433972 predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure igfbp3-tmem219-1 3433967 N/A 3433971 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false uni unidentified N/A 3433972 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false uniba unidentified baculovirus physical association physical association false false false Figure 2A igfbp3-tmem219-2 3433968 N/A 3433971 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3433972 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 2B tmem219-igfbp3-3 3433969 N/A 3433971 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate conditioned medium conditioned medium region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false uni unidentified N/A 3433972 unspecified role unspecified role prey prey purified purified uni unidentified physical association physical association false false false Figure 2 F tmem219-caspase8_chlae_protein-2 3433970 N/A 3433975 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell N/A 3433973 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Figure 5D RHS