IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2010-91 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Wang K., Long B., Zhou J., Li PF. peifli@ioz.ac.cn The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation wang-2010-92 human-cardiomyocytes human cardiomyocytes ac16 ac16 human cardiomyocyte cell line AC16 Human Cardiomyocyte Cell Line is a proliferating cell line that was derived from the fusion of primary cells from adult human ventricular heart tissues with SV40 transformed, uridine auxotroph human fibroblasts, devoid of mitochondrial DNA heart Heart [Cardiac; Cardiac tissue] ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Wang K., Long B., Zhou J., Li PF. peifli@ioz.ac.cn The Journal of biological chemistry 2010 Only protein-protein interactions imex curation imex curation hsa-mir-9-5p human micro rna 9 5p mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UCUUUGGUUAUCUAGCUGUAUGA mrna_myocardin myocardin mrna messenger rna mRNA mrna human Homo sapiens Human AATCGCCGGCAGCCTATGACATCAGACAGGAACGCCTGGGATGCCGCGCTGCTCCTGGCCAACCTCCGAGGAGGAGGAGGGTCCCGCCGGCTAAGAGTTAATTAGCCCCGCACGGCGAGGGGGGAGGCGCCAGTTTTCTGGGGACACTGGCTGCCACTGTACTCCTACCCAGGGGAGCTCACGGAGAGTTGGATGAATTCTGGGTTGTTAGCTGCGGTCAGCTGGGCTCCCGGGAGCCTGTTGCTGGTGGAGAACAGGGGGCGCCTGGCCAAGGGACCAGCGGCTTGCTGAGACTCAACATGACACTCCTGGGGTCTGAGCATTCCTTGCTGATTAGGAGCAAGTTCAGATCAGTTTTACAGTTAAGACTTCAACAAAGAAGGACCCAGGAACAACTGGCTAACCAAGGCATAATACCACCACTGAAACGTCCAGCTGAATTCCATGAGCAAAGAAAACATTTGGATAGTGACAAGGCTAAAAATTCCCTGAAGCGCAAAGCCAGAAACAGGTGCAACAGTGCCGACTTGGTTAATATGCACATACTCCAAGCTTCCACTGCAGAGAGGTCCATTCCAACTGCTCAGATGAAGCTGAAAAGAGCCCGACTCGCCGATGATCTCAATGAAAAAATTGCTCTACGACCAGGGCCACTGGAGCTGGTGGAAAAAAACATTCTTCCTGTGGATTCTGCTGTGAAAGAGGCCATAAAAGGTAACCAGGTGAGTTTCTCCAAATCCACGGATGCTTTTGCCTTTGAAGAGGACAGCAGCAGCGATGGGCTTTCTCCGGATCAGACTCGAAGTGAAGACCCCCAAAACTCAGCGGGATCCCCGCCAGACGCTAAAGCCTCAGATACCCCTTCGACAGGTTCTCTGGGGACAAACCAGGATCTTGCTTCTGGCTCAGAAAATGACAGAAATGACTCAGCCTCACAGCCCAGCCACCAGTCAGATGCGGGGAAGCAGGGGCTTGGCCCCCCCAGCACCCCCATAGCCGTGCATGCTGCTGTAAAGTCCAAATCCTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAGGACCCCAAGCCAAAGGTGAAGAAGCTTAAATATCACCAGTACATTCCCCCAGACCAGAAGGCAGAGAAGTCCCCTCCACCTATGGACTCAGCCTACGCTCGGCTGCTCCAGCAACAGCAGCTGTTCCTGCAGCTCCAAATCCTCAGCCAGCAGCAGCAGCAGCAGCAACACCGATTCAGCTACCTAGGGATGCACCAAGCTCAGCTTAAGGAACCAAATGAACAGATGGTCAGAAATCCAAACTCTTCTTCAACGCCACTGAGCAATACCCCCTTGTCTCCTGTCAAAAACAGTTTTTCTGGACAAACTGGTGTCTCTTCTTTCAAACCAGGCCCACTCCCACCTAACCTGGATGATCTGAAGGTCTCTGAATTAAGACAACAGCTTCGAATTCGGGGCTTGCCTGTGTCAGGCACCAAAACGGCTCTCATGGACCGGCTTCGACCCTTCCAGGACTGCTCTGGCAACCCAGTGCCGAACTTTGGGGATATAACGACTGTCACTTTTCCTGTCACACCCAACACGCTGCCCAATTACCAGTCTTCCTCTTCTACCAGTGCCCTGTCCAACGGCTTCTACCACTTTGGCAGCACCAGCTCCAGCCCCCCGATCTCCCCAGCCTCCTCTGACCTGTCAGTCGCTGGGTCCCTGCCGGACACCTTCAATGATGCCTCCCCCTCCTTCGGCCTGCACCCGTCCCCAGTCCACGTGTGCACGGAGGAAAGTCTCATGAGCAGCCTGAATGGGGGCTCTGTTCCTTCTGAGCTGGATGGGCTGGACTCCGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAGGTGATCAATGAACTCACCTGGAAACTCCAGCAAGAGCAGAGGCAGGTGGAGGAGCTGAGGATGCAGCTTCAGAAGCAGAAAAGGAATAACTGTTCAGAGAAGAAGCCGCTGCCTTTCCTGGCTGCCTCCATCAAGCAGGAAGAGGCTGTCTCCAGCTGTCCTTTTGCATCCCAAGTACCTGTGAAAAGACAAAGCAGCAGCTCAGAGTGTCACCCACCGGCTTGTGAAGCTGCTCAACTCCAGCCTCTTGGAAATGCTCATTGTGTGGAGTCCTCAGATCAAACCAATGTACTTTCTTCCACATTTCTCAGCCCCCAGTGTTCCCCTCAGCATTCACCGCTGGGGGCTGTGAAAAGCCCACAGCACATCAGTTTGCCCCCATCACCCAACAACCCTCACTTTCTGCCCTCATCCTCCGGGGCCCAGGGAGAAGGGCACAGGGTCTCCTCGCCCATCAGCAGCCAGGTGTGCACTGCACAGAACTCAGGAGCACACGATGGCCATCCTCCAAGCTTCTCTCCCCATTCTTCCAGCCTCCACCCGCCCTTCTCTGGAGCCCAAGCAGACAGCAGTCATGGTGCCGGGGGAAACCCTTGTCCCAAAAGCCCATGTGTACAGCAAAAGATGGCTGGTTTACACTCTTCTGATAAGGTGGGGCCAAAGTTTTCAATTCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAATTTCAGAGGTAACACAGCCTCCATCCTATGAAGATGCCGTAAAGCAGCAAATGACCCGGAGTCAGCAGATGGATGAACTCCTGGACGTGCTTATTGAAAGCGGAGAAATGCCAGCAGACGCTAGAGAGGATCACTCATGTCTTCAAAAAGTCCCAAAGATACCCAGATCTTCCCGAAGTCCAACTGCTGTCCTCACCAAGCCCTCGGCTTCCTTTGAACAAGCCTCTTCAGGCAGCCAGATCCCCTTTGATCCCTATGCCACCGACAGTGATGAGCATCTTGAAGTCTTATTAAATTCCCAGAGCCCCCTAGGAAAGATGAGTGATGTCACCCTTCTAAAAATTGGGAGCGAAGAGCCTCACTTTGATGGGATAATGGATGGATTCTCTGGGAAGGCTGCAGAAGACCTCTTCAATGCACATGAGATCTTGCCAGGCCCCCTCTCTCCAATGCAGACACAGTTTTCACCCTCTTCTGTGGACAGCAATGGGCTGCAGTTAAGCTTCACTGAATCTCCCTGGGAAACCATGGAGTGGCTGGACCTCACTCCGCCAAATTCCACACCAGGCTTTAGCGCCCTCACCACCAGCAGCCCCAGCATCTTCAACATCGATTTCCTGGATGTCACTGATCTCAATTTGAATTCTTCCATGGACCTTCACTTGCAGCAGTGGTAGAATGCCCAATGCACCAGTGCTATGGAAGACCAATGGAGTTCCATGGGGGAAAGCACACAGCCATACATACTTTACTGTCCAAAAACAGAAGAAGAAGAAGAGAATTAAAAAGAAGCAATGATTTCTGTGCCAATGAACAAGAACAAAAGTCATTTTTAGAAATACATATACTGTAATATTTACCAACAGTCAGTAACTGTTAATGATTTCAACAATGCATTAAAAGAATGTGCTTTCTCAGATTAAGGATGCCAAAAAAGATATTTCACTGCCTTTTCAAAGACCAGTATATTTTCTAGCCCATAATTTTTCTCAGGCATTGTTGGGGCATAAGCTCACACTGTAAGCTTTTCTCATGAATTCACTAGACATAACGTGGAAGGAAAACGTAGTCTTTTGGGAGTACAGGGAAGCCAGCCCCTCAAAGCTTATGGAAGACATACCTGCAATGGAAGCTGTTGCCCAATGTCTCCATTACTATCTTTCAAAAGAGAAGCCAGACCCAGCTTCAGATCAAAAGTTCTTGAGACAGAGGAACAAAACCAATCGATTTCCAGGGAAGCTAATCAACTCTCTTTTCCCTCTACCACAAAACTGCCCTGCTGGAGTGGTTCTGAACCTGTACCCAGGACTCGATGTGGTCACTAATAACAATTAACCTGAACTGAGTCCACAGAACTCCACTCGGAACTTTCTTCTTTTTTAACTAGTGGCCCAATCATTCCCACCATCTCTGTGCTGATAAGTACGTGTCCTAGATGAGAACCCTGAAGAATGCAGACCTTCTTCCCCCGAAGGAGATGCCACAAGCTCTCCAACACAGCCCCCTTTAGTTCCAAAGACTAGAGATGACCACATTGGTAGAAGTATATCTCGAGGCACAGGAAGGGAGCCCCACCAGGGATAATTCAGACAGGACTAGAGAATAACATCATTTCACATACCCTGGGATAAACACCCTGGGTTCCTATAGAAGGACTATTACTTATGGGAGTCCAACTTCTCCTTTTGTTTTGTTATTATCAGTTTATCTTTCTCCCACTCCACTTTTCCTTCAAGGTACCAATCCTTTCCTGTTCCTCGTTTGGCCATCTTTCTTTTTCTGCCTCCACATTGGGAGGGGAGGACTTCTCAGTTCTAACAAGCTGCCATACTCCTAAGAAAGCCATTTTTGAAAAATTTAACAATCCAGGTTCTTCTGGAGAACTCATTCTCCACACGCACAGTTTGCTGCAAAAGGAAGTTGCAAGAATTTCTTGAGGAAGAAACTGGTGACTTGGTCCATCAGTCACGAAGTTCTTTCTATTCTCGTTTAGTTTTCAAGAAATTATTGGTTTGTGTTGCTCTGGGGAAATTGGAAATCATTACATTGTAAAGACAAATATGGATGATATTTACAAGAGAGAATTTCAGATCTGGGTTTTTGAAAGAAAACAGAATTGCGCATTGAAAACGATGGAAGGAAAAAGACAATGGTCTAATGTGCATTCCTCATTACCTCTCGTGGCTTTGGCTGGGAGTTGGAAAAAGCTAAAATTTCAGAACAGTCTCTGTAAGGCTCTCTGTGGCTCCAGTTCACCATTTTATATTGTTGCATGCTGTAGAAAGGAGCTATTGCTGTTGTTTTGTTTTTTTATTTAAATCACTAAGGCACTGTTTTTATCTTTTGTAAAAAAAAAAAAAAAGTTGTTCACTGTGCACTTATAGAAAAAATAATCAAAAATGTTGGGATTTTAGAAGCTCTCTTTTTGATAAACCAAAGATTTAGAAGTCATTCCATTGTTAACTTGTAAAAATGTGTGAACACAGAGAGTTTTTGGTGATTGCTACTCTGAAAGCTGCCAGATCTTATTCTGGGGGTGGGATGTGGAGGAATACACATACACACACAAACATACATGTATGTATAATAGATATATACATATGTGTATATTATATCTGTGTGTGCATGTATCTCCAAAAGCGGCGTTACAGAGTTCTACACCAAAAGCCTTTAACCCTTAATCTGCTGTGAATGATACCTGGCCTTTCTCACTATGAATTTCTGATTAACCAACCAGACTACACGTTGCCTCTCTGTGTATGACTAACGGCTCCAACCCGATGACTCACAGCTACTTGCTTATCGTGAACAAGCTCATCTTGGCAATGAATATGGATGTGAAAAGACAGAACAGCTTCACCATTAGTAGCTGGAAATGGTATCACAGTCTCTTATAGAGGAATATGAAAGGAACAAGAAAATCATTTTACATTCCTTTTATCTGTATTGTGCTTTAAAAGATCCACATGGTAAATTTTTTATTTTGCTTTTATGTCAGTCATCAGAACCAAAAAAATCCAGAAGAAAAAATTGCCAGTGTTTCCTTTGAAGATGAAGCTACTGGGGAAGAAAACCTTATTAATACACTCCACACATTTGTTCATTCCTCAGCTGTTGGTGTTTTCTTGGGGTCTTGACAAAGCTTGCTGGTCAGTGCACTTTTCAGGTGTCACGTTTTGCTGTTTGTATGTTTTTTCTTCCCCTTACTTCCTTTGGAAAACAAACTCACACAGTGCCCCTACTCTGAGACCTGGGACTGAGTGTTAATTATTTTTTCCTTGGGTATTTCTATCTGAGAGACTAGACCTAGTTAGGAGGCCTCTGTACTTCTCCAGATTGTACCTTTTTATGGGGATCTTTGAGGCTATGACCCAGGACTGATAGATATGCCTTACGGAAGACAAAAGATAAAATGGTTCCTATATCCTAATGCAAACCAACACAGTTAAAAGAGCAGATCTCTGGATAACTGCTCTCAACCTGCTTCTACAGTCTCCACAAACCGCATTCACCCTCTCTCTTCATAGCTCAGACATGAAATTTGAGGGAGAAAACTGGAGATAATTGGGAGAAAATTGATGAAGTTGGCTGCTTCCAGTAGATCAGATAATCCATGAATTTGTCTCCCATTGAGAATTTTATTTTAAATTCTTTTAAACTCTTCGTTGTGTCTTTTGTGATGACAAATCAGGCATGACTAAAAGATGTACAGAGACTTACGAAGATGGTCACATTCAAGTTCCCTAATGCTCTTAGAACCTGAAGATGACCATGTGTAGTTTTCTTAAGACCTCTGAACCCCCATGGTGATGAAGACTTGAAGACATTTGCAGCTATCTGCTGCAGTCTGGTAGATTCATACTTATCTAAAGAAGTCAAAAAATTTATTCGTGCAAGTGCTTGCAGGAAGCCAGTGCTTATTAGTAGTGACCCTGCTTCTATCAACGTTATTGAGACAACACATATTCTATTCTAAGGGAGAAAGAGGGAGGAAGAGAGGGAGGGAGGGAGGAAGAAGAGGGAGGGAGCGAGGAAGGAAGATAGGAGATGGGTAGGGGGGTAAAGAGAAAAGGAGGGAGAAGGGAAGGAAGGAAAGAAGAGAGGAAAGAAAGGAGGGAAGGAAAAAAGGGCCAAACTTTCTGATCTATGAACTTCTCAGTTCAGCTGTCACATTATGAGAAGTAAATCAGAATTTTTTTAAGGAGAAGTCATTCTTAGCACTACAATAATTGTACCAGTAATTGAGGAAACCAAGACAATCTTCACCTGAATAATAGAGGGTCTGAGAACTGTCAGCCTTTTGCCATTCAAAAACATTTATGTCCAACCTGAAAAAAAAGCATCAATAAAACCTATCCCAAGCATTCAAAATAGTCCTTTCCAAATGTTATTTATTTTAAAGTCAATCAGCTCTTTTAGAAACAGATTCTGGTCTGGCTGAAAACTCCCACAACAAATTTACTCATCCAGTGGCTAATATTTAATGCCCACCATGGGCAGAGCACACAAATCTTCAGATAAACAATACTGAATTGATTCAGCACAGATTGTTCAGATTTGAATGACCAGGGAGTTGTATTTGCACATGCAAGAACACTAAGAATCTCCCAGTCCTCAAACTAGAAACCTTTCAGCTACGATGAAAAAAAAAAAGGGGTCTTCATTTTTCCAAGAGGGGGTGGAGGTGGGGATCACTTTTTAGCTAAAAGCTATCTCTCACTTCAAAATTCTTGTCTTTTTCTTTGTGGACAAACACCAGTAGTCTATCACTTGGAGATCTTTTAATATCTCCCATCATTTAAAACATCCACGAGAGTTTGAAGATTTGTGTTGATTGCCAGATACAGAAGCCCCTTGAAAATAAGGAAAGGGTGGAGGAAGCATTTTTGTGTCCTATCCCTACTTATCGTAGCAGCTCTATAGACAAAAGGGACACTTACTGGTGAGCCTCTGGCCCTTAAAAGAAAATCATCTAAGAATATGAAGGCAATTTGATTTCCCCCCACAGCCCTCAGCTGCCTTCCTCACAGAAGGAAGTTCCCAAAATTGCTGGTACACAGTTTGCAATCAAATATCAGATATGAGAAAACCTGTAGTGAAGAGTCTGGGTTCTTGGTTTTCTCATAAATCCAATATAAATTTGTAGGTTGGTTCAGGGTCAAAATTGCCAGTGCTTTATTAGACAGATGATACTGATAGACACACAGAGCCCAGGTCCTGGAACAAGACAATCCTGTAGTGCCAAGATCTGGTCAGTTGCGTTAAGGAGCTGGGTTTGATTCTAGAGTCCAGGTTTATAGAGAAACCCTGGCTAGATTGAGCCTACCCATGGGGAGACGATTTCAAGACAGGATGAGATCTGGGAAGAATTTTGTTGTCATCTGCCAGGGAAATTATCACAGGACTCATTGAATGCAATAACATGTGAGTAAGTTCCCTTTTGATTCTGGGAATCAGCGATTTTCCCTGTGGATTAAGACAAACCAACGCCAGAAGGTCTCCTGTGCTTATTTTAACCATCTGCTCCCATCGTGAACCCTGGAGCATGCATTTCCTAGAAGTGGTTTCATAGCTCCTGTGTGTTCATGGAAAAGGGGAGTATAATGATGGGGATGCTGGAAGCTTTTTTAATGTTTTCCAAAGGAAAGGAACCCACACTGCTCCCCAGAGTTCCTTTCCAATGGCCCTGCAGTAAGAACGGAGGACAATGTATTGCTGGGTGCTTAAAATCCTCCCTCAGTGAAGCACAAAGAGACACTTTGTAAAGAAAAAAAGAGCAAGCATAGGTTCTCTGTGGGACCTTGTGGAGTGGTGTTTTCACGTTGGTCTCTTTGGCTCAATTGAGCATAATCAGAAAGAAATGTGGGTTATTGGGAAGAGACAAAAAGCAGTGGCTAAAATACCAAAGTTGGCATGTGTTCTTTTTTAAAAAAAAAAAAAAATGCATATATTTTTAAATAAAATGTTTATTTTAAAAAGAAAAAAAAAAAAAAAAAA myocardin myocardin gene gene human Homo sapiens Human nfac3_human Nuclear factor of activated T-cells, cytoplasmic 3 NFATC3 NFAT4 NFATx T-cell transcription factor NFAT4 protein protein human Homo sapiens Human MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSPCGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSDDQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETEGSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESKIIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPVPHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVVYNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVADQITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPSSLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDVNEIIGRDMSQISVSQGAGVSRQAPLPSPESLDLGRSDGL hsa_mir_9_5p-mrna_myocardin-1 3475330 N/A 3475332 predetermined predetermined participant predetermined regulator regulator modulator regulator neutral component neutral component engineered engineered purified purified N/A 3475333 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false r.4097_4100delinsaggc mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 6A, D down-regulates quantity by destabilization nfatc3-1 3475331 N/A 3475334 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3475335 antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig3D Isoproterenol (CHEBI:64317); aldosterone (CHEBI:27584)