IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ blokhuis-2017-1 in vitro In vitro affinity techniques affinity technology affinity techniques antibody detection identification by antibody antibody detection test Alleles KIR2DS5*002, KIR2DS5*009, KIR2DS5*010 and KIR2DS5*011 have activating potential but do not recocognize (or with very slight avidity) C2 epitopes of HLA-C alleles. Assays using Luminex® bead-based multiplexing technology:color-coded microbeads coated with purified HLA antigens and tested for binding to KIR-Fc fusion proteins. A secondary PE conjugated antibody is added that binds to the Fc portion of the KIR–Fc. Binding was detected by fluorescence - either flow cytometry or fluorescence microscopy. Results > 0.04 relative to W632 antibody have been entered as positive results. A KIR2DS5*005 allele construct was used as a template for all other alleles. The sequence is stated to be "residues 1–224 of the mature protein";this has been assumed to be aas 22-245 - the ECD. Uniprot entry Q14953 (KIR2DS5) gives the sequence for allele KIR2DS5*002;all other alleles have been entered as variants/mutations of this sequence. lisbeth.guethlein@stanford.edu Immunity, inflammation and disease 2017 Only protein-protein interactions imex curation imex curation test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, ki2s5_human Killer cell immunoglobulin-like receptor 2DS5 KIR2DS5 CD158G NKAT9 CD158 antigen-like family member G MHC class I NK cell receptor Natural killer-associated transcript 9 protein protein human Homo sapiens Human MSLMVISMACVAFFLLQGAWPHEGFRRKPSLLAHPGPLVKSEETVILQCWSDVMFEHFLLHREGTFNHTLRLIGEHIDGVSKGNFSIGRMTQDLAGTYRCYGSVTHSPYQLSAPSDPLDIVITGLYEKPSLSAQPGPTVLAGESVTLSCSSRSSYDMYHLSREGEAHERRLPAGPKVNRTFQADFPLDPATHGGTYRCFGSFRDSPYEWSKSSDPLLVSVTGNSSNSWPSPTEPSSETGNPRHLHVLIGTSVVKLPFTILLFFLLHRWCSNKKNASVMDQGPAGNRTVNREDSDEQDHQEVSYA 1c02_human HLA class I histocompatibility antigen, Cw-2 alpha chain HLA-C HLAC MHC class I antigen Cw*2 protein protein human Homo sapiens Human MRVMEPRTLILLLSGALALTETWACSHSMRYFYTAVSRPSRGEPHFIAVGYVDDTQFVRFDSDAASPRGEPRGRWVEQEGPEYWDRETQKYNRQAQTDRVNLRKLRGYYNQSEAGSHTLQRMYGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEEWRAYLEGECVEWLRRYLENGKEKLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPTEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIASKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). hlac_human HLA class I histocompatibility antigen, C alpha chain HLA-Cw Human leukocyte antigen C HLAC HLA-C protein protein human Homo sapiens Human MRVMAPRALLLLLSGGLALTETWACSHSMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQADRVSLRNLRGYYNQSEDGSHTLQRMSGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKLEAARAAEQLRAYLEGTCVEWLRRYLENGKETLQRAEPPKTHVTHHPLSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHMQHEGLQEPLTLSWEPSSQPTIPIMGIVAGLAVLVVLAVLGAVVTAMMCRRKSSGGKGGSCSQAACSNSAQGSDESLITCKA hla_c-kir2ds5-1 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ser144asn variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. N/A 3314030 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false lys202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false ile10leu variant variant certain certain sequence position certain certain certain sequence position certain false gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain false glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Supplementary figure 1A-1 C*02:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-2 3314028 N/A 3314030 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false lys202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false ile10leu variant variant certain certain sequence position certain certain certain sequence position certain false gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain false glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg203his variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-2 C*02:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-3 3314028 N/A 3314030 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false lys202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false ile10leu variant variant certain certain sequence position certain certain certain sequence position certain false gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain false glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-3 C*02:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-4 3314028 N/A 3314030 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false lys202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false ile10leu variant variant certain certain sequence position certain certain certain sequence position certain false gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain false glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-4 C*02:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-5 3314028 N/A 3314030 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false lys202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false ile10leu variant variant certain certain sequence position certain certain certain sequence position certain false gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain false glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified glu237lys variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-5 C*02:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-6 3314028 N/A 3314030 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain false lys202thr variant variant certain certain sequence position certain certain certain sequence position certain false ser364cys variant variant certain certain sequence position certain certain certain sequence position certain false ile10leu variant variant certain certain sequence position certain certain certain sequence position certain false gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain false glu5ala variant variant certain certain sequence position certain certain certain sequence position certain false asn92lys variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-6 C*02:02 allele, KIR2DS5*008 allele hla_c-kir2ds5-7 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ser144asn variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-1a C*05:01 allele, KIR2DS5*003 allele hla_c-kir2ds5-8 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg203his variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-2a C*05:01 allele, KIR2DS5*004 allele hla_c-kir2ds5-9 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-3a C*05:01 allele, KIR2DS5*005 allele hla_c-kir2ds5-10 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-4a C*05:01 allele, KIR2DS5*006 allele hla_c-kir2ds5-11 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false glu237lys variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-5a C*05:01 allele, KIR2DS5*007 allele hla_c-kir2ds5-12 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-6a C*05:01 allele, KIR2DS5*008 allele hla_c-kir2ds5-13 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ser144asn variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-1b C*06:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-14 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg203his variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-2b C*06:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-15 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-3b C*06:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-16 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-4b C*06:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-17 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false glu237lys variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-5b C*06:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-18 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-6b C*06:02 allele, KIR2DS5*008 allele hla_c-kir2ds5-19 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ser144asn variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-1c C*15:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-20 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg203his variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-2c C*15:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-21 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-3c C*15:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-22 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-4c C*15:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-23 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false glu237lys variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-5c C*15:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-24 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-6c C*15:02 allele, KIR2DS5*008 allele hla_c-kir2ds5-25 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ser144asn variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-1d C*17:01 allele, KIR2DS5*003 allele hla_c-kir2ds5-26 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg203his variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-2d C*17:01 allele, KIR2DS5*004 allele hla_c-kir2ds5-27 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false Supplementary figure 1A-3d C*17:01 allele, KIR2DS5*005 allele hla_c-kir2ds5-28 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-4d C*17:01 allele, KIR2DS5*006 allele hla_c-kir2ds5-29 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false glu237lys variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-5d C*17:01 allele, KIR2DS5*007 allele hla_c-kir2ds5-30 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified N/A 3314029 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-6d C*17:01 allele, KIR2DS5*008 allele hla_c-kir2ds5-31 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ser144asn variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Supplementary figure 1A-1e C*18:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-32 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg203his variant variant certain certain sequence position certain certain certain sequence position certain false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Supplementary figure 1A-2e C*18:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-33 3314028 N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. n/a 3314031 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Supplementary figure 1A-3e C*18:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-34 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-4e C*18:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-35 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false glu237lys variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-5e C*18:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-36 3314028 n/a 3314031 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3314029 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain false ecd sufficient to bind sufficient binding region sufficient to bind range range range range false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arg179gly variant variant certain certain sequence position certain certain certain sequence position certain false trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction false false false Supplementary figure 1A-6e C*18:02 allele, KIR2DS5*008 allele