IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hu-2017-3 in vitro In vitro biosensor biosensor predetermined predetermined participant predetermined Hu T., Wang S., Chen C., Sun J., Yang X. Dual-polarization interferometry ( DPI ) is an analytical technique that probes molecular layers adsorbed to the surface of a waveguide using the evanescent wave of a laser beam. xryang@ciac.ac.cn,jiansun@ciac.ac.cn Analytical chemistry 2017 imex curation Only protein-protein interactions imex curation hu-2017-6 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hu T., Wang S., Chen C., Sun J., Yang X. xryang@ciac.ac.cn,jiansun@ciac.ac.cn Analytical chemistry 2017 imex curation Only protein-protein interactions imex curation hu-2017-4 in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Hu T., Wang S., Chen C., Sun J., Yang X. xryang@ciac.ac.cn,jiansun@ciac.ac.cn Analytical chemistry 2017 imex curation Only protein-protein interactions imex curation hu-2017-5 in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis western blot western blot Immuno blot Immuno blot Hu T., Wang S., Chen C., Sun J., Yang X. xryang@ciac.ac.cn,jiansun@ciac.ac.cn Analytical chemistry 2017 imex curation Only protein-protein interactions imex curation lirb2_human Leukocyte immunoglobulin-like receptor subfamily B member 2 LILRB2 ILT4 LIR2 MIR10 CD85 antigen-like family member D Immunoglobulin-like transcript 4 Monocyte/macrophage immunoglobulin-like receptor 10 protein protein human Homo sapiens Human MTPIVTVLICLGLSLGPRTHVQTGTIPKPTLWAEPDSVITQGSPVTLSCQGSLEAQEYRLYREKKSASWITRIRPELVKNGQFHIPSITWEHTGRYGCQYYSRARWSELSDPLVLVMTGAYPKPTLSAQPSPVVTSGGRVTLQCESQVAFGGFILCKEGEEEHPQCLNSQPHARGSSRAIFSVGPVSPNRRWSHRCYGYDLNSPYVWSSPSDLLELLVPGVSKKPSLSVQPGPVVAPGESLTLQCVSDVGYDRFVLYKEGERDLRQLPGRQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSECSAPSDPLDILITGQIRGTPFISVQPGPTVASGENVTLLCQSWRQFHTFLLTKAGAADAPLRLRSIHEYPKYQAEFPMSPVTSAHAGTYRCYGSLNSDPYLLSHPSEPLELVVSGPSMGSSPPPTGPISTPAGPEDQPLTPTGSDPQSGLGRHLGVVIGILVAVVLLLLLLLLLFLILRHRRQGKHWTSTQRKADFQHPAGAVGPEPTDRGLQWRSSPAADAQEENLYAAVKDTQPEDGVEMDTRAAASEAPQDVTYAQLHSLTLRRKATEPPPSQEREPPAEPSIYATLAIH p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 lilrb2-app-2 3038893 N/A 3038897 unspecified role unspecified role bait bait purified purified ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 3038898 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 direct interaction direct interaction false false false 3038893 Figure 3,4 Table 1, supp fig 5/6 (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) lilrb2-app-3 3038893 N/A 3038897 unspecified role unspecified role bait bait purified purified ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3038898 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 direct interaction direct interaction false false false 3038893 Figure 3,4 Table 1, Supp fig 5/6 (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) lilrb2-app-4 3038893 N/A 3038897 unspecified role unspecified role bait bait purified purified ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 3038898 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 14.0 direct interaction direct interaction false false false 3038893 Figure 3,4 Table 1, Supp Fig 5/6. (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) app-116 3038894 N/A 3038897 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. n/a 3038898 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 physical association physical association false false false Supp. figure 2 (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) All forms of Beta-amyloid protein 42 all bound to GFP-ED1D2L. lilrb2-app-1 3038894 N/A 3038897 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate ecd_D1D2_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3038898 unspecified role unspecified role prey prey purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 physical association physical association false false false Supp. figure 2i (Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.) All forms of Beta-amyloid protein 42 all bound to GFP-ED1D2L. app-114 3038895 n/a 3038898 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 direct interaction direct interaction false false false Figure 1 Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer. app-115 3038896 n/a 3038898 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Figure 1 Low molecular weight Beta-amyloid protein 42 oligomer (LMW) is a 2–4 mer, while the high molecular weight oligomer is a 14-48 mer.