DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hayes-2016-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hayes RP., Xiao Y., Ding F., van Erp PB., Rajashankar K., Bailey S., Wiedenheft B., Ke A. DIP original experiment: DIP-268649X Nature 2016 IMEx Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Hayes RP., Xiao Y., Ding F., van Erp PB., Rajashankar K., Bailey S., Wiedenheft B., Ke A. Only protein-protein interactions imex curation hayes-2016-2 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Hayes RP., Xiao Y., Ding F., van Erp PB., Rajashankar K., Bailey S., Wiedenheft B., Ke A. DIP original experiment: DIP-268651X Nature 2016 IMEx Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Hayes RP., Xiao Y., Ding F., van Erp PB., Rajashankar K., Bailey S., Wiedenheft B., Ke A. Only protein-protein interactions imex curation hayes-2016-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hayes RP., Xiao Y., Ding F., van Erp PB., Rajashankar K., Bailey S., Wiedenheft B., Ke A. DIP original experiment: DIP-268650X Nature 2016 IMEx Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Hayes RP., Xiao Y., Ding F., van Erp PB., Rajashankar K., Bailey S., Wiedenheft B., Ke A. Only protein-protein interactions imex curation cas6_ecoli CRISPR system Cascade subunit CasE casE CasE endoRNase cas6e crRNA endonuclease ygcH JW2726 b2756 protein protein ecoli Escherichia coli (strain K12) MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL cas5_ecoli CRISPR system Cascade subunit CasD casD cas5 ygcI b2757 JW5844 protein protein ecoli Escherichia coli (strain K12) MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACLGIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHETIQTWREYLCDASFTVALWLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLGTCQASDPQKALLNYEPVGGDIYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKGGMDVSQ casc_ecoli CRISPR system Cascade subunit CasC casC cas4 cse4 ygcJ JW2728 b2758 protein protein ecoli Escherichia coli (strain K12) MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSLKRAMRKSGYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKSVDEAEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDIALSGRMATSGMMTELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQEFSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPADMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSLSDVDPITAQVKQMPTLEQLKSWVRNNGEA cse2_ecoli CRISPR system Cascade subunit CasB casB cse2 ygcK JW2729 b2759 protein protein ecoli Escherichia coli (strain K12) MADEIDAMALYRAWQQLDNGSCAQIRRVSEPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSEQTTGISLGRALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA cse1_ecoli CRISPR system Cascade subunit CasA casA cse1 ygcL JW2730 b2760 CRISPR type I-E/Ecoli-associated protein CasA/Cse1 CRISPR-associated protein CasA/Cse1 protein protein ecoli Escherichia coli (strain K12) MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGGGFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPNESHTENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTALRKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG casa-casb-14 3289232 N/A 3289235 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289236 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289237 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289239 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-casa-6 3289233 N/A 3289235 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3289236 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3289237 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3289238 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3289239 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false casa-casb-15 3289234 N/A 3289235 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289236 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289237 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289238 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289239 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false