DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mo-2016-1 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. DIP original experiment: DIP-269012X Nature chemical biology 2016 IMEx Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. Only protein-protein interactions imex curation mo-2016-2 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. DIP original experiment: DIP-269018X Nature chemical biology 2016 IMEx Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. Only protein-protein interactions imex curation mo-2016-3 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. DIP original experiment: DIP-269017X Nature chemical biology 2016 IMEx Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. Only protein-protein interactions imex curation mo-2016-4 in vitro In vitro acetylase assay acetylase assay predetermined predetermined participant predetermined Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. DIP original experiment: DIP-269015X Nature chemical biology 2016 IMEx Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. Only protein-protein interactions imex curation mo-2016-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. DIP original experiment: DIP-269013X Nature chemical biology 2016 IMEx Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. Only protein-protein interactions imex curation mo-2016-6 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. DIP original experiment: DIP-269016X Nature chemical biology 2016 IMEx Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. Only protein-protein interactions imex curation mo-2016-7 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. DIP original experiment: DIP-269014X Nature chemical biology 2016 IMEx Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation. Mo F., Zhuang X., Liu X., Yao PY., Qin B., Su Z., Zang J., Wang Z., Zhang J., Dou Z., Tian C., Teng M., Niu L., Hill DL., Fang G., Ding X., Fu C., Yao X. Only protein-protein interactions imex curation kat5_human Histone acetyltransferase KAT5 KAT5 TIP60 HTATIP Lysine acetyltransferase 5 cPLA(2)-interacting protein Histone acetyltransferase HTATIP 60 kDa Tat-interactive protein protein protein human Homo sapiens Human MAEVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA 2aaa_human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform PPP2R1A PP2A subunit A isoform PR65-alpha PP2A subunit A isoform R1-alpha Medium tumor antigen-associated 61 kDa protein protein protein human Homo sapiens Human MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA h3k9-1 H3K9 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKA aurkb-kat5-1 3288712 N/A 3288719 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human N/A 3288720 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human physical association physical association false false false ppp2r1a-aurkb-1 3288713 N/A 3288721 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3288720 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false acK215 acK215 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false PTM disrupting an interaction ecolx Escherichia coli physical association physical association false false false aurkb-ppp2r1a-1 3288714 N/A 3288721 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3288720 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96GD4:p.Lys215Gln K215Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false aurkb-kat5-2 3288715 N/A 3288720 enzyme target enzyme target substrate substrate unspecified role unspecified role acK215 acK215 naclys N-acetylated L-lysine NAcLys western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false Resulting PTM Q96GD4:p.Lys215Arg K215R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3288719 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli acetylation acetylation reaction acetylation false false false kat5-aurkb-1 3288716 N/A 3288720 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3288719 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false aurkb-h3(115)-1 3288717 N/A 3288722 enzyme target enzyme target substrate substrate unspecified role unspecified role pS10 pS10 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false Resulting PTM GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3288720 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false aurkb-kat5-3 3288718 N/A 3288720 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3288719 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false