DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ketel-2016-1 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. DIP original experiment: DIP-268606X Nature 2016 IMEx A phosphoinositide conversion mechanism for exit from endosomes. Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. Only protein-protein interactions imex curation ketel-2016-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. DIP original experiment: DIP-268610X Nature 2016 IMEx A phosphoinositide conversion mechanism for exit from endosomes. Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. Only protein-protein interactions imex curation ketel-2016-3 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. DIP original experiment: DIP-268608X Nature 2016 IMEx A phosphoinositide conversion mechanism for exit from endosomes. Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. Only protein-protein interactions imex curation ketel-2016-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. DIP original experiment: DIP-268611X Nature 2016 IMEx A phosphoinositide conversion mechanism for exit from endosomes. Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. Only protein-protein interactions imex curation ketel-2016-5 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. DIP original experiment: DIP-268607X Nature 2016 IMEx A phosphoinositide conversion mechanism for exit from endosomes. Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. Only protein-protein interactions imex curation ketel-2016-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. DIP original experiment: DIP-268609X Nature 2016 IMEx A phosphoinositide conversion mechanism for exit from endosomes. Ketel K., Krauss M., Nicot AS., Puchkov D., Wieffer M., Müller R., Subramanian D., Schultz C., Laporte J., Haucke V. Only protein-protein interactions imex curation mtm1_human Myotubularin MTM1 CG2 Phosphatidylinositol-3,5-bisphosphate 3-phosphatase Phosphatidylinositol-3-phosphate phosphatase protein protein human Homo sapiens Human MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLITDKEVIYICPFNGPIKGRVYITNYRLYLRSLETDSSLILDVPLGVISRIEKMGGATSRGENSYGLDITCKDMRNLRFALKQEGHSRRDMFEILTRYAFPLAHSLPLFAFLNEEKFNVDGWTVYNPVEEYRRQGLPNHHWRITFINKCYELCDTYPALLVVPYRASDDDLRRVATFRSRNRIPVLSWIHPENKTVIVRCSQPLVGMSGKRNKDDEKYLDVIRETNKQISKLTIYDARPSVNAVANKATGGGYESDDAYHNAELFFLDIHNIHVMRESLKKVKDIVYPNVEESHWLSSLESTHWLEHIKLVLTGAIQVADKVSSGKSSVLVHCSDGWDRTAQLTSLAMLMLDSFYRSIEGFEILVQKEWISFGHKFASRIGHGDKNHTDADRSPIFLQFIDCVWQMSKQFPTAFEFNEQFLIIILDHLYSCRFGTFLFNCESARERQKVTERTVSLWSLINSNKEKFKNPFYTKEINRVLYPVASMRHLELWVNYYIRWNPRIKQQQPNPVEQRYMELLALRDEYIKRLEELQLANSAKLSDPPTSPSSPSQMMPHVQTHF exoc4_human Exocyst complex component 4 EXOC4 KIAA1699 SEC8 SEC8L1 Exocyst complex component Sec8 protein protein human Homo sapiens Human MAAEAAGGKYRSTVSKSKDPSGLLISVIRTLSTSDDVEDRENEKGRLEEAYEKCDRDLDELIVQHYTELTTAIRTYQSITERITNSRNKIKQVKENLLSCKMLLHCKRDELRKLWIEGIEHKHVLNLLDEIENIKQVPQKLEQCMASKHYLSATDMLVSAVESLEGPLLQVEGLSDLRLELHSKKMNLHLVLIDELHRHLYIKSTSRVVQRNKEKGKISSLVKDASVPLIDVTNLPTPRKFLDTSHYSTAGSSSVREINLQDIKEDLELDPEENSTLFMGILIKGLAKLKKIPETVKAIIERLEQELKQIVKRSTTQVADSGYQRGENVTVENQPRLLLELLELLFDKFNAVAAAHSVVLGYLQDTVVTPLTQQEDIKLYDMADVWVKIQDVLQMLLTEYLDMKNTRTASEPSAQLSYASTGREFAAFFAKKKPQRPKNSLFKFESSSHAISMSAYLREQRRELYSRSGELQGGPDDNLIEGGGTKFVCKPGARNITVIFHPLLRFIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVLAEINKEIEGVTKTSDPLKILANADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSAYSDQFLNMVCVKLQEYKDTCTAAYRGIVQSEEKLVISASWAKDDDISRLLKSLPNWMNMAQPKQLRPKREEEEDFIRAAFGKESEVLIGNLGDKLIPPQDILRDVSDLKALANMHESLEWLASRTKSAFSNLSTSQMLSPAQDSHTNTDLPPVSEQIMQTLSELAKSFQDMADRCLLVLHLEVRVHCFHYLIPLAKEGNYAIVANVESMDYDPLVVKLNKDISAIEEAMSASLQQHKFQYIFEGLGHLISCILINGAQYFRRISESGIKKMCRNIFVLQQNLTNITMSREADLDFARQYYEMLYNTADELLNLVVDQGVKYTELEYIHALTLLHRSQTGVGELTTQNTRLQRLKEIICEQAAIKQATKDKKITTV pi42a_mouse Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha PtdIns(5)P-4-kinase isoform 2-alpha Diphosphoinositide kinase 2-alpha Pip4k2a Pip5k2a 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha Phosphatidylinositol 5-phosphate 4-kinase type II alpha Phosphatidylinositol 5-Phosphate 4-Kinase Pi5p4ka protein protein mouse Mus musculus Mouse MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYVIKTITSEDVAEMHNILKKYHQYIVECHGVTLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKIFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDSTHPIGTPPDSPGNTLNSSPPLAPGEFDPNIDVYAIKCHENAPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHIL exoc7_human Exocyst complex component 7 EXOC7 EXO70 KIAA1067 Exocyst complex component Exo70 protein protein human Homo sapiens Human MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENLQRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVKLLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYGRNQDFMNVYYQIRSSQLDRSIKGLKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGTIRKAQNLLKQYSQHGLDGKKGGSNLIPLEGLLPCTPRGGLPGPWINAACVCAADISPGHEHDFRVKHLSEALNDKHGPLAGRDDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDGLMLEGENIVSAARKAIVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGTAASTKNKLPGLITSMETIGAKALEDFADNIKNDPDKEYNMPKDGTVHELTSNAILFLQQLLDFQETAGAMLASQETSSSATSYSSEFSKRLLSTYICKVLGNLQLNLLSKSKVYEDPALSAIFLHNNYNYILKSLEKSELIQLVAVTQKTAERSYREHIEQQIQTYQRSWLKVTDYIAEKNLPVFQPGVKLRDKERQIIKERFKGFNDGLEELCKIQKAWAIPDTEQRDRIRQAQKTIVKETYGAFLQKFGSVPFTKNPEKYIKYGVEQVGDMIDRLFDTSA pip4k2a-exoc4-1 3287872 N/A 3287878 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3287879 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3287880 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false mtm1-exoc4-1 3287873 N/A 3287879 unspecified role unspecified role prey prey B10-tag B10-tag fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3287878 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false exoc4-pip4k2a-1 3287874 N/A 3287879 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3287880 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false exoc4-mtm1-1 3287875 N/A 3287878 unspecified role unspecified role prey prey B10-tag B10-tag fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey N/A 3287879 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false mtm1-pip4k2a-1 3287876 N/A 3287878 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3287880 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association false false false pip4k2a-exoc4-2 3287877 N/A 3287881 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3287879 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3287880 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false