DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ poepsel-2015-1 in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268148X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-2 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268154X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-3 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268156X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-4 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight by comassie molecular weight estimation by coomasie staining weight by comassie Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268152X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-5 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268157X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-6 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268149X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268155X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-8 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268159X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-9 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight silver stain molecular weight estimation by silver staining weight silver stain Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268158X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-10 in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268150X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-11 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268153X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation poepsel-2015-12 in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268151X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Only protein-protein interactions imex curation p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL htra1_human Serine protease HTRA1 HTRA1 HTRA PRSS11 High-temperature requirement A serine peptidase 1 L56 Serine protease 11 protein protein human Homo sapiens Human MQIPRAALLPLLLLLLAAPASAQLSRAGRSAPLAAGCPDRCEPARCPPQPEHCEGGRARDACGCCEVCGAPEGAACGLQEGPCGEGLQCVVPFGVPASATVRRRAQAGLCVCASSEPVCGSDANTYANLCQLRAASRRSERLHRPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMITVIPEEIDP p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 mapt-1 3284504 N/A 3284516 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false htra1-mapt-1 3284505 N/A 3284517 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3284516 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage false false false htra1-mapt-2 3284506 N/A 3284517 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role DyLight488-label DyLight488-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false htra1-mapt-3 3284507 N/A 3284517 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role Region 258-360 Region 258-360 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false abeta(142)-18 3284508 N/A 3284518 unspecified role unspecified role unspecified role unspecified role in vitro In vitro N/A 3284518 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction false false false mapt-2 3284509 N/A 3284516 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false htra1-mapt-4 3284510 N/A 3284517 unspecified role unspecified role unspecified role unspecified role Region 380-480 Region 380-480 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role Region 385-393 Region 385-393 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 308-319 Region 308-319 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false mapt-3 3284511 N/A 3284516 unspecified role unspecified role unspecified role unspecified role DyLight488-label DyLight488-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role DyLight488-label DyLight488-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false abeta(142)-htra1-1 3284512 N/A 3284517 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284518 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction false false false htra1-mapt-5 3284513 N/A 3284517 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false mapt-4 3284514 N/A 3284516 unspecified role unspecified role unspecified role unspecified role Region 258-360 Region 258-360 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role Region 258-360 Region 258-360 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false htra1-mapt-6 3284515 N/A 3284517 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3284516 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false