DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ baker-2015-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266296X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-4 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266301X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266303X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266298X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-7 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266300X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-8 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266304X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-9 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266302X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-10 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266297X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation baker-2015-11 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. DIP original experiment: DIP-266299X Science (New York, N.Y.) 2015 IMEx A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Baker RW., Jeffrey PD., Zick M., Phillips BP., Wickner WT., Hughson FM. Only protein-protein interactions imex curation g0s5g3_chatd V-SNARE coiled-coil homology domain-containing protein CTHT_0032860 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MASSSTSTPLLYACIAYETTILTETTLSPSSHASSLSSLALPKIDHSKPQKATYAREPYHIHYLASSASEYPDDEASAGRLTYLVISPDKTLYRVTCFEFLTTLRTRFLARFPPEKHDFSNFLPYGCAEFEGELRKLMAEYSQRALDVENNGGDSINSVQREIEDVRGIMSRNIEGLLERGERIDLLVDKTDRLGGSAREFRLRSRGLKRKMWWKNVKLMGLLGFVVVLILLTIVTSVKGGV g0s236_chatd CTHT_0015850 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MSFDQLSSLEAGRRRGPTTSSRYTDDPEFARLSQDLMNKLFRLNGNNQRLAGEIGHLGTRRDTPRVRERVHELIEESRDMFKQVGEGVKKIQAWEDVTPTQRYMQQKLSREFQTSLAEFQSLQRQALEKQKASVSAARASFESEHHAPSATGAPEQHQLLQQQQELTHLAPQDEVDFQEALILEREEEIRNIEQGVSDLNVLFQQVAQLVAEQGEVLDTIERNVEAVGDDTRGADRELRAAARYQKRARSRMCCLLMILTVILTIILLAIFVG g0scm5_chatd CTHT_0057760 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MAGQFAAKRLGKELQKIRNSLPPGIELVSADNFEEWLLDIRVLDDNPLYANQTYRLRFRFGSTYPIEPPEVVFVKSADRPIPIHPHIYSNGIICLDLLGHQGWSPVQNVESVCMSLQTPRLPTPTWIHNHQTPHGGLVSMAPRAGFDAEQVRDKARKDLLHLLEGVRGKKNLVIEKDLAGPLGVIVKASTLRDYGVDNFFFLENKNTGTSQRNIVFIARGESVRNAHAIAAQIKRIQRESQTSHDFHIFWVPRRTLFSDKVLEEAGVLGDANISELPLYFFPLERDVLSLELNDSFRDLYLAKDPTPVFLLSRALMGIQKKHGLFPRIIGKGENAKRVADLLSRMRQELLAGEEAGESDRAGLSPSTTIESVIIIDREVDFVTPLLTQLTYEGLIDEYFGIQNNQTDVDAVIVGAPAQSAASTSTAVPTNSSQSRKRKIQLDGSDSLYSQLRDANFAIVGSLLNTVARRLKSDYESRHNTKTTAELKEFVKKLPGYQAEQQSLKIHSNIAEEIINYTRTEIFNKLLEVQQNLAAGADPSSQFDSIEELVARDTPLPQVLRLLCLYSCISGGIKTKELDHFRRLVLQGYGHQHLLTLHNLERLQMFLSKSSPLASMITMSGSSGGPDQKTNYTYLRKQLRLIVDEVNEQDPNDIAYVYSGYAPLSIRLVQCVLQKQYLLSITKGSGTVIAAGPVAGGGAQGWKGFEEIVKHARGPTFDEIQKGEDKAVKARALLSGSSGDKKTVFVVFVGGITFTEIAALRFIAKQEEARRNIVICTTSIINGNRMMNAAIETATFEKTTVTTAAAQ g0s6m7_chatd Probable vacuolar protein sorting-associated protein 16 homolog CTHT_0026760 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MDTAHPTASWEQLGERFYRKIQLYTQVFDQDFDLDNYIVTGAPYGGAIALYRDDEKLVAYQPSRSSRPTIDICSLSGKLLRRISWDQGPIKGVGWSEDEKLLIVMVDGTVRCYFDLQSEFTQFSLGHGAEEHGVKSCRFYSHGLVALLGNNALVSVSSYDEPRPKLLASPPEGRVYSWNIIPPAYSLSRSVEVLLSVNQTIYVCDASECEDRFLDIGPFSHIAVSPNGRFCALYTTTGKVHVITSDFQSRLSEHDTKSKIAPNYFEWCGNDAVVIAWDDEVHLVGPSGSLARFFYDSGRIHLIPDFDGVRILANDRCDFLQKVPDVIEEVFGLGADSPASILLDAVEQLEMKSPKADDNIQLIRPHLVEAVDTCVSAAGQEFSIHWQKQLLKAASFGKSVLDIYNSDDFVDMCETLRVLNAVRFYEVGLPLSYEQYQRLSPSGLISRLLNRHEYLLAIRIADHLRLPTDKIHVHWASAKVRLGSEDDDTICRKIVEKLSGKPGISFEVIARTAYEEGRTRLATELLNHEPRAGRQVPLLLSMEEDELALDKAIESGDTDLIYFVIHQLRRKLPLASFFRVVSSRPTASAMVEALARNSDGDGNEDTALLKDLYYQDDRRLDGASVFIREALQQPETRTASDKLDLAANLLQGNQKEHVFELGALKEAKMLLLNQTMFKLIKLGYHGRAKKIQSEFKVPERVAWWIRLQALVAKRDWNEIEEISRQRKSPIGWEPFFNQVLQAGNPRLAATFIPKCTNLEPGQTITMYEKCGMRVKAAEEAVRLKDTEAWNRLLEAAGRNTAEGREIERLGATVFKK g0s236_chatd-g0s5g3_chatd-1 3283381 N/A 3283390 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283391 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false g0s6m7_chatd-g0scm5_chatd-4 3283382 N/A 3283393 unspecified role unspecified role unspecified role unspecified role Region503-816 Region503-816 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false g0s236_chatd-g0scm5_chatd-1 3283383 N/A 3283391 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false Deletion of region 476-498 decreased the interaction rate/strength. g0s5g3_chatd-g0scm5_chatd-1 3283384 N/A 3283390 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false g0s236_chatd-g0s6m7_chatd-1 3283385 N/A 3283391 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283393 unspecified role unspecified role unspecified role unspecified role Region 503-816 Region 503-816 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false g0s5g3_chatd-g0scm5_chatd-2 3283386 N/A 3283390 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G0S5G3:p.Phe201Ala F201A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false g0s5g3_chatd-g0scm5_chatd-3 3283387 N/A 3283390 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false G0SCM5:p.Gly460Asp G460D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SCM5:p.Ala467His A467H mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false G0SCM5:p.Phe489Ala F489A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false G0SCM5:p.Ser507Glu S507E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Region 476-498 Region 476-498 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false g0s236_chatd-g0scm5_chatd-2 3283388 N/A 3283391 unspecified role unspecified role unspecified role unspecified role Region 181-245 Region 181-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false g0s5g3_chatd-g0s6m7_chatd-1 3283389 N/A 3283393 unspecified role unspecified role unspecified role unspecified role Region 503-816 Region 503-816 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283390 unspecified role unspecified role unspecified role unspecified role Region 148-212 Region 148-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283392 unspecified role unspecified role unspecified role unspecified role Region 139-806 Region 139-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false