DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ xiao-2015-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267282X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions imex curation xiao-2015-5 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267285X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions imex curation xiao-2015-6 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267287X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions imex curation xiao-2015-7 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267283X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions imex curation xiao-2015-8 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267284X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions imex curation xiao-2015-9 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267286X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions imex curation q9h0e2-1 Toll-interacting protein TOLLIP protein protein human Homo sapiens Human MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP tom1_human Target of Myb protein 1 TOM1 protein protein human Homo sapiens Human MDFLLGNPFSSPVGQRIEKATDGSLQSEDWALNMEICDIINETEEGPKDALRAVKKRIVGNKNFHEVMLALTVLETCVKNCGHRFHVLVASQDFVESVLVRTILPKNNPPTIVHDKVLNLIQSWADAFRSSPDLTGVVTIYEDLRRKGLEFPMTDLDMLSPIHTPQRTVFNSETQSGQDSVGTDSSQQEDSGQHAAPLPAPPILSGDTPIAPTPEQIGKLRSELEMVSGNVRVMSEMLTELVPTQAEPADLELLQELNRTCRAMQQRVLELIPQIANEQLTEELLIVNDNLNNVFLRHERFERFRTGQTTKAPSEAEPAADLIDMGPDPAATGNLSSQLAGMNLGSSSVRAGLQSLEASGRLEDEFDMFALTRGSSLADQRKEVKYEAPQATDGLAGALDARQQSTGAIPVTQACLMEDIEQWLSTDVGNDAEEPKGVTSEEFDKFLEERAKAADRLPNLSSPSAEGPPGPPSGPAPRKKTQEKDDDMLFAL ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tollip-tom1-4 3283153 N/A 3283159 unspecified role unspecified role unspecified role unspecified role Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 54-182 Region 54-182 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283160 unspecified role unspecified role unspecified role unspecified role Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tom1-ub-1 3283154 N/A 3283161 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3283160 unspecified role unspecified role unspecified role unspecified role Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tollip-tom1-5 3283155 N/A 3283159 unspecified role unspecified role unspecified role unspecified role Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283160 unspecified role unspecified role unspecified role unspecified role Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tom1-tollip-1 3283156 N/A 3283159 unspecified role unspecified role prey prey Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283160 unspecified role unspecified role bait bait Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O60784:p.Glu223Ala E223A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O60784:p.Met226Ala M226A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O60784:p.Val227Ala V227A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O60784:p.Asn230Ala N230A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O60784:p.Leu257Ala L257A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O60784:p.Cys261Ser C261S mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false O60784:p.Met264Ala M264A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O60784:p.Arg267Ala R267A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tom1-tollip-4 3283157 N/A 3283159 unspecified role unspecified role prey prey Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9H0E2-1:p.[Arg9Ala;Val12Ala] R9A/V12A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9H0E2-1:p.[Asp20Ala;Arg23Ala] D20A/R23A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9H0E2-1:p.Phe21Ala F21A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 54-182 Region 54-182 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3283160 unspecified role unspecified role bait bait Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ub-tom1-1 3283158 N/A 3283161 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3283160 unspecified role unspecified role prey prey Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false