DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chug-2015-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266430X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266424X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-3 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266431X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-4 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266432X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-5 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266428X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-6 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266426X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-7 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266427X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-8 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266429X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation chug-2015-9 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. DIP original experiment: DIP-266425X Science (New York, N.Y.) 2015 IMEx Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex. Chug H., Trakhanov S., Hülsmann BB., Pleiner T., Görlich D. Only protein-protein interactions imex curation nup93_xenla Nuclear pore complex protein Nup93 nup93 93 kDa nucleoporin An4a Nucleoporin Nup93 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDGEGFGELLQQAEQLAAETEGVTELPHVERNLQEIQQAGERLRSKTMTRTSQESANVKASVLLGSRGLDISHISQRLESLSAATTFEPLEPVKDTDIQGFLKNEKDNALLSAIEESRKRTFVMAEEYHRESMLVEWEQVKQRVLHTLLASGEDALDFTQESETSYISESGAPGRSSLDNVEMAYARQMYMYNEKVVSGHLQPSLVDLCTEAAERLDDKNVSDLWVMVKQMTDVPLIPASDTLKSRCSGQMQMAFVRQALNYLEQSYKNYTLISVFANLQQAQLGGVPGTYNLVRSFLNIRLPTPIPGLQDGEIEGYPVWALIYYCMRCGDLMAAQQVVNRAQHQLGDFKNCFQEYIHNKDRRLSPTTENKLRLHYRRAVRASTDPYKRAVYCIIGRCDVSDNHSEVADKTEDYLWLKLSQVCFEDEANSSPQDRLTLPQFQKQLFEDYGESHFAVNQQPYLYFQVLFLTAQFEAAIAFLFRLERTRCHAVHVALALFELKLLLKSTGQSAQLLSQEPGEPQGVRRLNFIRLLMLYTRKFEPTDPREALQYFYFLRNEKDNQGESMFLRCVSELVIESREFDMLLGKLEKDGSRKPGAIDKFTRDTKTIINKVASVAENKGLFEEAAKLYDLAKNPDKVLELTNKLLSPVVSQISAPQSNRERLKNMALAIAERYKSQGVSAEKSINSTFYLLLDLITFFDEYHAGHIDLSFDVIERLKLVPLSQDSVEERVAAFRNFSDEIRHNLSEILLATMNILFTQYKRLKGSGPTTLGRPQRVQEDKDSVLRSQARALITFAGMIPYRMSGDTNARLVQMEVLMN q91349_xenla nup62.L IL4I1 nup62 XELAEV_18038559mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGFNFGAASAGGFSFGNPKSTTTTAPTGFSFGAATAAPSGGFSFGTATPTPASTTGQTSGLFSFSNPAPSLAPTSGFSFGAQVTSTPAPSSGGLAFGANTSKLNSGVGNQPAGGTTQTSQPMGGFSFGAATTQTQPSATSVGGFSFAGGVGSTSTNVFAQPAASTGITLQSAVSTAAAPTATTSQPTSTFSFGTQPQAAPALNFGLLSSSSVLSTASTPAAAQPVAPTTGLSLNFGKPADTSAAVTSTGSTTTNTPSLSSLLGTSGPSLFSSVATSTVPSVVSTVASGLSLTSTATSTGFGMKTLASSAVPTGTLATSTASLGVKAPLAGTIVQANAVGSAAATGISTATAMTYAQLENLINKWSLELEDQEKHFLQQATQVNAWDRTLMQNGERITTLHREMEKVKLDQKRLDQELDFILSQQKELEDLLTPLEESVKEQSGTIYLQHADEEREKTYKLAENIDAQLKRMAQDLKEVIEHLNTSAGPGDASNPLQQICKILNAHMDSLQWIDQNSALLQRKVEQVTKECESRRKEQERGFSIAFD q5eax5_xenla nup58.S MGC84997 nup58 XELAEV_18016222mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MASGFSFGTAAASTTTLNPTAAAPFSFGATPAASNTGTTGGLGFGAFNAAATPATTTATTGLGGGLFGAKPAAGFTLGGANTATATTTAASTGFSVGFNKPAGSATPFSLPVTSTSSGGLSLASALTSTPATGPSPFTLNLGSTPATTTAAATGLSLGGTLTGLGGSLFQNTNPSATGLGQSTLGQSTLGQSTLGQSLLGQSLLGQSLLGQSTLGQSTLGQSLLGQSLLGLGLNLGAVAPVSQVTTHEGLGGLDFSSSSDKKSDKAGTRPEDSKALKDENLPQLLCQDVENFQKFVKEQKQVQEEISRMSSKAMLKVQEDIKALKQLLSVASSGLQRNALAIDKLKIETAEELKNAEIALRTQKTPPGLQHENTAPADYFHTLVQQFEVQLQQYRQQIEELENHLATQSNTLHLSPQDLSMAMQKLYQTFVALAAQLQAVNENFKMLKEQYLGYRKAFLGDSTDVFEARRAEAKKWQNAPRVTTGPTPFSNIPNAAAVAMAATLTQQQQPTTGFGSSSAFGGNTSGSSSFGFGTANKPSGSLSAGFGSTSTSGFNFSNPGINASAGLTFGVSNPSSTSFGTGQLLQLKKPPAGNKRGKR k9ztj6_xenla nup54.S nup54 XELAEV_18009519mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAFNFGATTGTPANQGTTGFSLGTFTPKTTTSGFGFGTTTTTAPTGFGGGFGGFGATTTASTGPAFSFTTPANTTSGLFGATQNKGFGFGTGFGSTTTSTGLGTGLGTGLGFTGFNTSQQQQQQSVLGAGLFNQSFQSTPQSNQLINTASALSAPTLLGDERDAILAKWNQLQAFWGTGKGFFMNNTPPVEFTQENPFCRFKAVGFSYIPNNKDEDGLISLIFNKKESDIRGQQQQLVESLHKVLGGHQTLTVNVEGVKTKADNQTEVIIYVVERSPNGTSRRVGASALFSYFEQAHIKANMQQLGVTGAMAQTELSPVQIKQLIQNPLSGVDPIIWEQAKVDNPDPERLIPVPMIGFKELLRRLEVQDQMTKQHQSRLDIISEDIGELQKNQTTTMAKIGQYKRKLMELSHRVLQVLIKQEIQRKSGFAIQAEEEQLRVQLDTIQSELNAPTQFKGRLNELMSQIRMQNHFGAVRSEEKYYVDADLLREIKQHLKQQQEGVSHLISIIKDNHEDIKLIEQGLNDNLHMRTGFLS nup93-k9ztj6_xenla-1 3282452 N/A 3282461 unspecified role unspecified role bait bait Region 1-95 Region 1-95 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3282462 unspecified role unspecified role prey prey Region 342-547 Region 342-547 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role prey prey Region 267-490 Region 267-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role prey prey Region 146-535 Region 146-535 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 474-535 Region 474-535 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false k9ztj6_xenla-nup58-1 3282453 N/A 3282462 unspecified role unspecified role unspecified role unspecified role Region 342-488 Region 342-488 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role unspecified role unspecified role Region 267-407 Region 267-407 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role unspecified role unspecified role Region 146-473 Region 146-473 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nup93-k9ztj6_xenla-2 3282454 N/A 3282461 unspecified role unspecified role bait bait Region 1-95 Region 1-95 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3282462 unspecified role unspecified role prey prey Region 342-547 Region 342-547 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role prey prey Region 267-490 Region 267-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 408-490 Region 408-490 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role prey prey Region 146-535 Region 146-535 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false nup93-k9ztj6_xenla-3 3282455 N/A 3282461 unspecified role unspecified role bait bait Region 1-95 Region 1-95 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3282462 unspecified role unspecified role prey prey Region 342-547 Region 342-547 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 489-547 Region 489-547 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role prey prey Region 267-490 Region 267-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role prey prey Region 146-535 Region 146-535 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false k9ztj6_xenla-nup62-1 3282456 N/A 3282462 unspecified role unspecified role prey prey Region 342-547 Region 342-547 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role bait bait Region 146-535 Region 146-535 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false nup62-k9ztj6_xenla-1 3282457 N/A 3282462 unspecified role unspecified role bait bait Region 342-547 Region 342-547 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q91349:p.[Gln411Arg;Gln425Arg] Q411R/Q425R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role prey prey Region 267-490 Region 267-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role prey prey Region 146-535 Region 146-535 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false k9ztj6_xenla-nup58-2 3282458 N/A 3282462 unspecified role unspecified role prey prey Region 342-547 Region 342-547 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role prey prey Region 267-490 Region 267-490 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role bait bait Region 146-535 Region 146-535 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false k9ztj6_xenla-nup58-3 3282459 N/A 3282462 unspecified role unspecified role unspecified role unspecified role Region 342-488 Region 342-488 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role unspecified role unspecified role Region 267-407 Region 267-407 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role unspecified role unspecified role Region 146-473 Region 146-473 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false k9ztj6_xenla-nup58-4 3282460 N/A 3282462 unspecified role unspecified role unspecified role unspecified role Region 342-488 Region 342-488 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282463 unspecified role unspecified role unspecified role unspecified role Region 267-407 Region 267-407 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282464 unspecified role unspecified role unspecified role unspecified role Region 146-473 Region 146-473 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false