DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yuan-2015-7 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266492X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266487X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-9 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266478X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-10 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266483X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-11 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266479X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-12 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266493X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-13 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266488X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-14 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266495X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-15 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266480X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-16 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266484X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-17 human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266485X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-18 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266489X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-19 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266481X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-20 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266491X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-21 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266482X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-22 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266490X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-23 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266486X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation yuan-2015-24 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. DIP original experiment: DIP-266494X Nature cell biology 2015 IMEx Deubiquitylase OTUD3 regulates PTEN stability and suppresses tumorigenesis. Yuan L., Lv Y., Li H., Gao H., Song S., Zhang Y., Xing G., Kong X., Wang L., Li Y., Zhou T., Gao D., Xiao ZX., Yin Y., Wei W., He F., Zhang L. Only protein-protein interactions imex curation otud3_human OTU domain-containing protein 3 OTUD3 KIAA0459 protein protein human Homo sapiens Human MSRKQAAKSRPGSGSRKAEAERKRDERAARRALAKERRNRPESGGGGGCEEEFVSFANQLQALGLKLREVPGDGNCLFRALGDQLEGHSRNHLKHRQETVDYMIKQREDFEPFVEDDIPFEKHVASLAKPGTFAGNDAIVAFARNHQLNVVIHQLNAPLWQIRGTEKSSVRELHIAYRYGEHYDSVRRINDNSEAPAHLQTDFQMLHQDESNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNAEENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQWMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG p60484-1 Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN PTEN MMAC1 TEP1 55kDa Mutated in multiple advanced cancers 1 Phosphatase and tensin homolog protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV otud3_mouse OTU domain-containing protein 3 Otud3 protein protein mouse Mus musculus Mouse MSRKQAAKSRPGSGGRRAEAERKRDERAARRALAKERRNRPDPGGSGCEEEFVSFANQLQALGLKLREVPGDGNCLFRALGDQLEGHSRNHLKHRQETVDYMIRQREDFEPFVEDDIPFEKHVASLSKPGTFAGNDAIVAFARNHQLNVVIHQLNAPLWQIRGTDKGSTRELHIAYRYGEHYDSVRRINDNSEAPAHLLTDFQMLHQDGANKKEKMKTKGVDVKDGLRDDVEDAVHKVGSATGCTDFNLIVQNLEAENYNIKSAITALLQVNQGTGNDAEENHEPGDRVKQRGPSREEAGSGRRLSGNQGRNEGRMETSEARASPAEESKAHKSQLPKVTNKQRREQQRLEKKKRQEERHRLKALENRNGSRDTGRSEADMNTQVTLVKTFAALNI pten_mouse Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Mutated in multiple advanced cancers 1 Pten Mmac1 Phosphatase and tensin homolog protein protein mouse Mus musculus Mouse MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV ubp13_human Ubiquitin carboxyl-terminal hydrolase 13 Ubiquitin-specific-processing protease 13 Deubiquitinating enzyme 13 Isopeptidase T-3 USP13 ISOT3 Ubiquitin thioesterase 13 protein protein human Homo sapiens Human MQRRGALFGMPGGSGGRKMAAGDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEGGLYVCMNTFLAFGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDLDTDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSKSPYRKQDPDTWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLWLNLTDGSVLCGKWFFDSSGGNGHALEHYRDMGYPLAVKLGTITPDGADVYSFQEEEPVLDPHLAKHLAHFGIDMLHMHGTENGLQDNDIKLRVSEWEVIQESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEFQRAYVGNLPRIFDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQQNGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSDVFRFLVEERIQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG otud3-otud3-1 3282320 N/A 3282338 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3282338 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pten-hub-1 3282321 N/A 3282339 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3282340 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pten-otud3-1 3282322 N/A 3282338 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3282340 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false pten-otud3-2 3282323 N/A 3282340 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 188-403 Region 188-403 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3282338 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false otud3-pten-1 3282324 N/A 3282338 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3282340 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false pten-otud3-3 3282325 N/A 3282340 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3282338 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q5T2D3:p.Arg96Lys R96K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q5T2D3:p.Arg144Ile R144I mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q5T2D3:p.Gly180Val G180V mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false pten-otud3-4 3282326 N/A 3282341 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3282342 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false usp13-pten-1 3282327 N/A 3282343 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3282340 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false pten-otud3-5 3282328 N/A 3282340 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3282338 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-183 Region 1-183 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false pten-hub-2 3282329 N/A 3282339 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli N/A 3282340 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false hub-pten-1 3282330 N/A 3282339 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3282340 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false otud3-pten-2 3282331 N/A 3282341 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3282342 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false otud3-pten-3 3282332 N/A 3282338 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3282340 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false otud3-pten-4 3282333 N/A 3282340 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3282338 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false otud3-pten-5 3282334 N/A 3282338 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-183 Region 1-183 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3282340 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false pten-mub-1 3282335 N/A 3282344 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3282342 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false pten-hub-3 3282336 N/A 3282339 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3282340 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false otud3-pten-6 3282337 N/A 3282340 unspecified role unspecified role prey prey Region 188-403 Region 188-403 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3282338 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false