DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ panvert-2015-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267208X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267217X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267213X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267209X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-5 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267214X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267219X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-7 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267218X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-8 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267210X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-9 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267220X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-10 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267215X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-11 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267216X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-12 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267211X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-13 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267221X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation panvert-2015-14 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. DIP original experiment: DIP-267212X Structure (London, England : 1993) 2015 IMEx Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features. Panvert M., Dubiez E., Arnold L., Perez J., Mechulam Y., Seufert W., Schmitt E. Only protein-protein interactions imex curation if2g_yeast Eukaryotic translation initiation factor 2 subunit gamma YER025W GCD11 TIF213 SUI4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLQDQEPSIIINGNLEPVGEPDIVEETEVVAQETQETQDADKPKKKVAFTGLEEDGETEEEKRKREFEEGGGLPEQPLNPDFSKLNPLSAEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQEPTCPEPDCYRSFKSDKEISPKCQRPGCPGRYKLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMREESALEHQKSILKFIRGTIADGAPIVPISAQLKYNIDAVNEFIVKTIPVPPRDFMISPRLIVIRSFDVNKPGAEIEDLKGGVAGGSILNGVFKLGDEIEIRPGIVTKDDKGKIQCKPIFSNIVSLFAEQNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPNIYTDIEINYFLLRRLLGVKTDGQKQAKVRKLEPNEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEINEKIALSRRIEKHWRLIGWATIKKGTTLEPIA D498AE62BC3E81CD cd123_schpo Cell division cycle protein 123 cdc123 SPAP27G11.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTLILTKNQVLHCQFSSWYSLFRKLTPKAKVIKPIPATVLKYLHEDSIYVEQPMNTVEEVDSEEDEESAPAYYPEREAIQLIEKAIKELGGAVVPKLNWSTPKDALWITTTGSLKCTTAEEVLLLLKSSDFVAHDLNHAFDDCKDFDNADGSVPKDFSFELVLKEWFPMHASTEFRCFVKSKRLIAFCQRDDNYYEFLKENIDCYEKLISDLLKKLDTFPDPDFVFDVYIHKDRAWLIDINPFYPRTDGLLFSWSELESMNSENMKPEIRLIPKGSMPSTGSAKYYTNRVPFDMIAASEGENLLEFAQKWQDLTNKSNE 352B195577263030 cd123_yeast Cell division cycle protein 123 CDC123 YLR215C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSQEYTTFIDIPVTRAQVEHCSYSFWSSLYPKYVPKSIVLKSLPKKFIQYLEQDGIKLPQEENSRSVYTEEIIRNEDNDYSDWEDDEDTATEFVQEVEPLIDFPELHQKLKDALNELGAVAPKLNWSAPRDATWILPNNTMKCNEVNELYLLLNASNYIMHDLQRAFKGCVDGDDIKGLKFDLVLRQWCDMNPALEFRVFVKNAHIVGATQRDLNYYDYLDELSDTFKDLIDEIVHDVVLPKFPDKSFVLDVYIPRPFNKIFIVDINPFARKTDSLLFSWNEIAAIAPPKNDVEDYELRLVTRHNTGRFASKEHSENHVPQDLVEASLNPEAIRELTQKWKELLSQQAKEESSDSENET 61CCC9DCEAFA1197 if2b_yeast Eukaryotic translation initiation factor 2 subunit beta YPL237W SUI3 TIF212 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSDLAAELGFDPALKKKKKTKKVIPDDFDAAVNGKENGSGDDLFAGLKKKKKKSKSVSADAEAEKEPTDDIAEALGELSLKKKKKKTKDSSVDAFEKELAKAGLDNVDAESKEGTPSANSSIQQEVGLPYSELLSRFFNILRTNNPELAGDRSGPKFRIPPPVCLRDGKKTIFSNIQDIAEKLHRSPEHLIQYLFAELGTSGSVDGQKRLVIKGKFQSKQMENVLRRYILEYVTCKTCKSINTELKREQSNRLFFMVCKSCGSTRSVSSIKTGFQATVGKRRRM 34DE9323876CEE95 if2a_yeast Eukaryotic translation initiation factor 2 subunit alpha SUI2 TIF211 YJR007W J1429 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTSHCRFYENKYPEIDDIVMVNVQQIAEMGAYVKLLEYDNIEGMILLSELSRRRIRSIQKLIRVGKNDVAVVLRVDKEKGYIDLSKRRVSSEDIIKCEEKYQKSKTVHSILRYCAEKFQIPLEELYKTIAWPLSRKFGHAYEAFKLSIIDETVWEGIEPPSKDVLDELKNYISKRLTPQAVKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQVKVKLVAAPLYVLTTQALDKQKGIEQLESAIEKITEVITKYGGVCNITMPPKAVTATEDAELQALLESKELDNRSDSEDDEDESDDE AF4F1C80303A4E98 sceif2gamma-spcdc123-1 3281094 N/A 3281108 unspecified role unspecified role unspecified role unspecified role Region 410-527 Region 410-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3281109 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sccdc123-sceif2gamma-1 3281095 N/A 3281110 unspecified role unspecified role bait bait Region 1-291 Region 1-291 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281108 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false sccdc123-1 3281096 N/A 3281110 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sceif2gamma-spcdc123-2 3281097 N/A 3281108 unspecified role unspecified role unspecified role unspecified role Region 410-527 Region 410-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3281109 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sceif2gamma-spcdc123-3 3281098 N/A 3281108 unspecified role unspecified role unspecified role unspecified role Region 410-527 Region 410-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3281109 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false sceif2_beta-sceif2gamma-1 3281099 N/A 3281111 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281108 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false sceif2_alpha-sceif2gamma-1 3281100 N/A 3281112 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281108 unspecified role unspecified role prey prey Region 325-331 Region 325-331 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false spcdc123-sceif2gamma-1 3281101 N/A 3281108 unspecified role unspecified role prey prey Region 410-527 Region 410-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3281109 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false sccdc123-sceif2gamma-2 3281102 N/A 3281110 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-291 Region 1-291 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q05791:p.[Asp266Ala;Ile267Ala;Asn268Ala] D266A/I267A/N268A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281108 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false sceif2gamma-sceif2_alpha-1 3281103 N/A 3281112 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281108 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false sccdc123-sceif2_alpha-1 3281104 N/A 3281110 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281112 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281108 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast association association false false false sccdc123-sceif2gamma-3 3281105 N/A 3281110 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3281108 unspecified role unspecified role unspecified role unspecified role Region 410-527 Region 410-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sccdc123-sceif2gamma-4 3281106 N/A 3281110 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3281108 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Deletion of region 325-331 decreased the interaction rate/strength. sccdc123-sceif2gamma-5 3281107 N/A 3281110 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3281108 unspecified role unspecified role unspecified role unspecified role Region 410-527 Region 410-527 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false