DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ woodruff-2015-1 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266227X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-2 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266232X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-3 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266230X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-4 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266228X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-5 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266235X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-6 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266233X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-7 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266231X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-8 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266229X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation woodruff-2015-9 in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. DIP original experiment: DIP-266234X Science (New York, N.Y.) 2015 IMEx Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro. Woodruff JB., Wueseke O., Viscardi V., Mahamid J., Ochoa SD., Bunkenborg J., Widlund PO., Pozniakovsky A., Zanin E., Bahmanyar S., Zinke A., Hong SH., Decker M., Baumeister W., Andersen JS., Oegema K., Hyman AA. Only protein-protein interactions imex curation spd5_caeel Spindle-defective protein 5 spd-5 F56A3.4 protein protein caeel Caenorhabditis elegans MEDNSVLNEDSNLEHVEGQPRRSMSQPVLNVEGDKRTSSTSATQQQVLSGAFSSADVRSIPIIQTWEENKALKTKITILRGELQMYQRRYSEAKEASQKRVKEVMDDYVDLKLGQENVQEKMEQYKLMEEDLLAMQSRIETSEDNFARQMKEFEAQKHAMEERIKELELSATDANNTTVGSFRGTLDDILKKNDPDFTLTSGYEERKINDLEAKLLSEIDKVAELEDHIQQLRQELDDQSARLADSENVRAQLEAATGQGILGAAGNAMVPNSTFMIGNGRESQTRDQLNYIDDLETKLADAKKENDKARQALVEYMNKCSKLEHEIRTMVKNSTFDSSSMLLGGQTSDELKIQIGKVNGELNVLRAENRELRIRCDQLTGGDGNLSISLGQSRLMAGIATNDVDSIGQGNETGGTSMRILPRESQLDDLEESKLPLMDTSSAVRNQQQFASMWEDFESVKDSLQNNHNDTLEGSFNSSMPPPGRDATQSFLSQKSFKNSPIVMQKPKSLHLHLKSHQSEGAGEQIQNNSFSTKTASPHVSQSHIPILHDMQQILDSSAMFLEGQHDVAVNVEQMQEKMSQIREALARLFERLKSSAALFEEILERMGSSDPNADKIKKMKLAFETSINDKLNVSAILEAAEKDLHNMSLNFSILEKSIVSQAAEASRRFTIAPDAEDVASSSLLNASYSPLFKFTSNSDIVEKLQNEVSELKNELEMARTRDMRSPLNGSSGRLSDVQINTNRMFEDLEVSEATLQKAKEENSTLKSQFAELEANLHQVNSKLGEVRCELNEALARVDGEQETRVKAENALEEARQLISSLKHEENELKKTITDMGMRLNEAKKSDEFLKSELSTALEEEKKSQNLADELSEELNGWRMRTKEAENKVEHASSEKSEMLERIVHLETEMEKLSTSEIAADYCSTKMTERKKEIELAKYREDFENAAIVGLERISKEISELTKKTLKAKIIPSNISSIQLVCDELCRRLSREREQQHEYAKVMRDVNEKIEKLQLEKDALEHELKMMSSNNENVPPVGTSVSGMPTKTSNQKCAQPHYTSPTRQLLHESTMAVDAIVQKLKKTHNMSGMGPELKETIGNVINESRVLRDFLHQKLILFKGIDMSNWKNETVDQLITDLGQLHQDNLMLEEQIKKYKKELKLTKSAIPTLGVEFQDRIKTEIGKIATDMGGAVKEIRKK plk1_caeel Serine/threonine-protein kinase plk-1 plk-1 plc1 C14B9.4 Polo-like kinase 1 protein protein caeel Caenorhabditis elegans MNRLPNIAKPPQKSNQRKEKAPPEVPALIADKDRGTYYEKGRFLGKGGFAHCYELTNRATREVVAGKVVPKSMLVKQYQRDKVDNERILIHRELGHINIVKLFNFFEDNLNVYITLELCARRSLMELHKRRKAVTEPEARYFTHQIVDGVLYLHDLNIIHRDMKLGNLFLNDDLVVKIGDFGLATTVNGDERKKTLCGTPNYIAPEVLNKAGHSFEVDIWAVGCILYILLFGQPPFESKSLEETYSRIRHNNYTIPSIATQPAASLIRKMLDPEPTRRPTAKQVQRDGFFKSGFMPTRLPVSCLTMVPKFGGHETSMMEENVAPRGVDARSQRPLNGRAGLSALPQHIVSNNADRERAQQQAAEATFREPEDAYLSQLFHQVAVLLEQRIPGLEEEEAALDGYQSPECLPVFWISKWVDYSDKYGIGYQLCDNSVGVLFNDNSRIMLDQAGNELTYIEKSNKEHYFSMHSGEMPGLLNKKVTLLKYFRSYMNDHLVKAGEGSEQRAGDDLARLPTLRVWFRTKSAIVLHLSNGTVQINFFNDHVKMMMCPLMQAVTFIDQNKRMLTYKLNNLQRNGCPEKFLHRLKYAKTMIERLMSDANVVSQNPARQPDMPRSMAAARSASAGSRGPNQAASHLPQSASGSNIHPRR zyg9_caeel Zygote defective protein 9 zyg-9 F22B5.7 protein protein caeel Caenorhabditis elegans MSNWDYLDEVDILPKLPPNFDELRESKKWQERKEALEALLKVLTDNERLSTKASYAELIGHLQMVLAKDANINCQALAAKCIGKFATGLRAKFSSFAGPLLPVIFEKMKEKKPMLREPLVDCSNEVGRTMQSLETGQEDILAALAKPNPQIKQQTALFVARQLDLVVPAKQPKGFIKAVVPVFGKLTGDADQDVREASLQGLGAVQRIIGDKNVKNLLGDASSDEGKMKKIGEYAEKSTASFAEEQAKNAPPVAPTSSTPSASAASGDPSGGTATAVVSSGAPVAEADPWDFLDAFDVLSKMPDGFDTNIESKKWQERKEALEGLLQLITANPKLDPKANYGALVERLQKVLEKDANINVAALAANCITGIANGLRTKFQPFAVSVTPIIFEKFKEKKPTLRDPLVACIDAVVATTNLEAVGEIVLAALGKPNPSIKTQTDLFLQRCFMKLNSQTMPKKTLKTLIPSLIKHSGDSDSEVREASYAAMGAMMRAIGEKPSLQLLADIASDNLKMSKIKEFHQKALDEAGPAEIAEMVKSIHKADAPPAAAPPKKTAPPKKQPEDEEVVEEEDEPLKPPPGDKKKKVPVKENEENEPPVVAPKAELLLSDNEDKKQRIKEEKQLKLVKWNFQAPTDEHISQLQTLLGNQAKVSLMSQLFHKDFKQHLAALDSLVRLADTSPRSLLSNSDLLLKWCTLRFFETNPAALIKVLELCKVIVELIRDTETPMSQEEVSAFVPYLLLKTGEAKDNMRTSVRDIVNVLSDVVGPLKMTPMLLDALKSKNARQRSECLLVIEYYITNAGISPLKSLSVEKTVAPFVGDKDVNVRNAAINVLVACFKFEGDQMWKAAGRMADKDKSLVEERIKRTGVKPGSGVVTSPPTGGPKILVPQQQGSVVRRPASRSRTREPEPEEVQSDTFTIRQDTMPPKTSSRYALRDDVFSSAMGRLDGTQVITPPQPVNGWSNNTFQMKRTNSSSSISSIDTSDQIQRSINNISSSLADVAQDAMFQVTYVLNQPEQRHLVDRRADLVFRASAAQLDLVIEEFNAGRDVSGTMDACTQMLFILMGGVETEHGLEPLNASPDTVKAIISSVLRCIIQIGNTESGYGMARSLNRLAMRLIYRVELSNLLCGLILAMTESLQMNTGITELVSKLSSKWCDELEKRRAQLRASDIVDSFNAFYVCALTELKMDISDSHILIVDNYLERVILQQGDVVLDAARRLSRPHMHLTSMINKILQMMRERKIDPIMPGTLEARMPQEDEAVVVRSGVQVSIDNILRDTSMAVKHIEQLNILIASSDRSWNEYMEYLKNNPMGELIKELVGECSRKKRIDFNLSHVVKSSMAVFKAMAATGPVQEEGRITPTDINRMDTMIVGTPLSRGDATITRARGNMIRPKRTTLSRDQMANIRHTLDRVKNH spd2_caeel Spindle-defective protein 2 F32H2.3 spd-2 protein protein caeel Caenorhabditis elegans MEDDAPMNLCNEQFEEIEDSPIDDNDNESFYNADGDVELEEEEVHETPKNFKNGGRFKTNMTNPKVNDLTTIEEKNEDLRSAASSRSASRPASVMSDKSFSSQFEFQSGGENAIEEYTNQVFADENKADLLFPETSKFMNGASPPKDKHHSWEPSIHHYDKQPPPDIQTNSPVFGNLNHRKNKLIPQARAKPGANDNEIVERDNDENVPTTSDKSAFITSPMNSTNHDEKTSTPKRPTNRKIGQYQGPNFDLSSIYVGSPQHQNTSISTGQQMPTSSYSHAHSETMMTNQTINESMVRRVLNGNNKNQDLFAALEEARKRRAAQPSKPDFRINTTRTRVPIKPTSARHSGNVVSSTSNDNTTAASSKDLTTSRKAMETFRQNASMADATNSNTASMTSILSTISTARTDISRSSRNHGGGFSNTSVSTVIPANNGNVSLSHGRDGRDSVSSVRTMSRASSTSTVYAGSTFSGVSKPLRIHAKRVAFGCVAVGETLRVEVEVENISDRQCLVRASTDSTTPVYQILDNKLTMVDPKKSIKFQVSFSPSSVGRYQVIMSIEVPAQNFIHKIPMWGNGGIAKFVPTSPDLQQTINQSEYAMCTSCAKRISFKISNSAGTRDGFAMIKVFDSAMRQLPDGCVAFFPAPGFIVKKKSDKRVDIRIDSSYIDLHDENNFRTSSSLSTASTTSSFQRRILPGAKFFVHVVWGEETMRTRLRLLEVRTGQHQLIDGHDFTSFQFSDEEVLRAVPVGFPAIKPEDRDLFAASYRSFFINFFTSTTEFRAATSRKKKEICSNDDSTLLETTAFRNQTFVNDVTIVPNTRFSNRK spd_5-1 3278971 N/A 3278980 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false spd_5-2 3278972 N/A 3278980 unspecified role unspecified role unspecified role unspecified role RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false plk_1-spd_5-1 3278973 N/A 3278981 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3278980 unspecified role unspecified role unspecified role unspecified role GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false spd_5-3 3278974 N/A 3278980 unspecified role unspecified role unspecified role unspecified role GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false spd_5-zyg_9-1 3278975 N/A 3278982 unspecified role unspecified role unspecified role unspecified role GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3278980 unspecified role unspecified role unspecified role unspecified role RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false plk_1-spd_5-2 3278976 N/A 3278981 unspecified role unspecified role unspecified role unspecified role GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3278980 unspecified role unspecified role unspecified role unspecified role RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false spd_2-spd_5-2 3278977 N/A 3278983 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3278980 unspecified role unspecified role unspecified role unspecified role GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false plk_1-spd_5-3 3278978 N/A 3278981 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3278980 enzyme target enzyme target substrate substrate unspecified role unspecified role P91349:p.Ser530Ala S530A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false pS530 pS530 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false spd_2-spd_5-3 3278979 N/A 3278983 unspecified role unspecified role unspecified role unspecified role GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3278980 unspecified role unspecified role unspecified role unspecified role RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false