DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ggl-2015-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. DIP original experiment: DIP-265410X PLoS biology 2015 IMEx The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism. Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. Only protein-protein interactions imex curation ggl-2015-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. DIP original experiment: DIP-265411X PLoS biology 2015 IMEx The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism. Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. Only protein-protein interactions imex curation ggl-2015-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. DIP original experiment: DIP-265413X PLoS biology 2015 IMEx The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism. Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. Only protein-protein interactions imex curation ggl-2015-4 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. DIP original experiment: DIP-265412X PLoS biology 2015 IMEx The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism. Gógl G., Schneider KD., Yeh BJ., Alam N., Nguyen Ba AN., Moses AM., Hetényi C., Reményi A., Weiss EL. Only protein-protein interactions imex curation mob2_yeast CBK1 kinase activator protein MOB2 YFL035C YFL035C-A YFL034C-B MOB2 MPS1 binder 2 Maintenance of ploidy protein MOB2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFFNFKAFGRNSKKNKNQPLNVAQPPAMNTIYSSPHSSNSRLSLRNKHHSPKRHSQTSFPAQKSTPQSQQLTSTTPQSQQQEASERSESQQIMFLSEPFVRTALVKGSFKTIVQLPKYVDLGEWIALNVFEFFTNLNQFYGVVAEYVTPDAYPTMNAGPHTDYLWLDANNRQVSLPASQYIDLALTWINNKVNDKNLFPTKNGLPFPQQFSRDVQRIMVQMFRIFAHIYHHHFDKIVHLSLEAHWNSFFSHFISFAKEFKIIDRKEMAPLLPLIESFEKQGKIIYN CFBA15FF83BFAA33 cbk1_yeast Serine/threonine-protein kinase CBK1 CBK1 YNL161W N1727 Cell wall biosynthesis kinase protein protein yeast Saccharomyces cerevisiae Baker's yeast MYNSSTNHHEGAPTSGHGYYMSQQQDQQHQQQQQYANEMNPYQQIPRPPAAGFSSNYMKEQGSHQSLQEHLQRETGNLGSGFTDVPALNYPATPPPHNNYAASNQMINTPPPSMGGLYRHNNNSQSMVQNGNGSGNAQLPQLSPGQYSIESEYNQNLNGSSSSSPFHQPQTLRSNGSYSSGLRSVKSFQRLQQEQENVQVQQQLSQAQQQNSRQQQQQLQYQQQQQQQQQQQHMQIQQQQQQQQQQQQSQSPVQSGFNNGTISNYMYFERRPDLLTKGTQDKAAAVKLKIENFYQSSVKYAIERNERRVELETELTSHNWSEERKSRQLSSLGKKESQFLRLRRTRLSLEDFHTVKVIGKGAFGEVRLVQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAERDVLAGSDSPWVVSLYYSFQDAQYLYLIMEFLPGGDLMTMLIRWQLFTEDVTRFYMAECILAIETIHKLGFIHRDIKPDNILIDIRGHIKLSDFGLSTGFHKTHDSNYYKKLLQQDEATNGISKPGTYNANTTDTANKRQTMVVDSISLTMSNRQQIQTWRKSRRLMAYSTVGTPDYIAPEIFLYQGYGQECDWWSLGAIMYECLIGWPPFCSETPQETYRKIMNFEQTLQFPDDIHISYEAEDLIRRLLTHADQRLGRHGGADEIKSHPFFRGVDWNTIRQVEAPYIPKLSSITDTRFFPTDELENVPDSPAMAQAAKQREQMTKQGGSAPVKEDLPFIGYTYSRFDYLTRKNAL 87EBCD2C3C96EE11 ssd1_yeast Protein SSD1 SSD1 CLA1 RLD1 SRK1 YDR293C D9819.4 Protein SRK1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKNSNVNNNRSQEPNNMFVQTTGGGKNAPKQIHVAHRRSQSELTNLMIEQFTLQKQLEQVQAQQQQLMAQQQQLAQQTGQYLSGNSGSNNHFTPQPPHPHYNSNGNSPGMSAGGSRSRTHSRNNSGYYHNSYDNNNNSNNPGSNSHRKTSSQSSIYGHSRRHSLGLNEAKKAAAEEQAKRISGGEAGVTVKIDSVQADSGSNSTTEQSDFKFPPPPNAHQGHRRATSNLSPPSFKFPPNSHGDNDDEFIATSSTHRRSKTRNNEYSPGINSNWRNQSQQPQQQLSPFRHRGSNSRDYNSFNTLEPPAIFQQGHKHRASNSSVHSFSSQGNNNGGGRKSLFAPYLPQANIPELIQEGRLVAGILRVNKKNRSDAWVSTDGALDADIYICGSKDRNRALEGDLVAVELLVVDDVWESKKEKEEKKRRKDASMQHDLIPLNSSDDYHNDASVTAATSNNFLSSPSSSDSLSKDDLSVRRKRSSTINNDSDSLSSPTKSGVRRRSSLKQRPTQKKNDDVEVEGQSLLLVEEEEINDKYKPLYAGHVVAVLDRIPGQLFSGTLGLLRPSQQANSDNNKPPQSPKIAWFKPTDKKVPLIAIPTELAPKDFVENADKYSEKLFVASIKRWPITSLHPFGILVSELGDIHDPDTEIDSILRDNNFLSNEYLDQKNPQKEKPSFQPLPLTAESLEYRRNFTDTNEYNIFAISELGWVSEFALHVRNNGNGTLELGCHVVDVTSHIEEGSSVDRRARKRSSAVFMPQKLVNLLPQSFNDELSLAPGKESATLSVVYTLDSSTLRIKSTWVGESTISPSNILSLEQLDEKLSTGSPTSYLSTVQEIARSFYARRINDPEATLLPTLSLLESLDDEKVKVDLNILDRTLGFVVINEIKRKVNSTVAEKIYTKLGDLALLRRQMQPIATKMASFRKKIQNFGYNFDTNTADELIKGVLKIKDDDVRVGIEILLFKTMPRARYFIAGKVDPDQYGHYALNLPIYTHFTAPMRRYADHVVHRQLKAVIHDTPYTEDMEALKITSEYCNFKKDCAYQAQEQAIHLLLCKTINDMGNTTGQLLTMATVLQVYESSFDVFIPEFGIEKRVHGDQLPLIKAEFDGTNRVLELHWQPGVDSATFIPADEKNPKSYRNSIKNKFRSTAAEIANIELDKEAESEPLISDPLSKELSDLHLTVPNLRLPSASDNKQNALEKFISTTETRIENDNYIQEIHELQKIPILLRAEVGMALPCLTVRALNPFMKRV 3D50FD6838ECA514 ace2_yeast Metallothionein expression activator ACE2 L3123 L9606.10 YLR131C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNVVDPWYINPSGFAKDTQDEEYVQHHDNVNPTIPPPDNYILNNENDDGLDNLLGMDYYNIDDLLTQELRDLDIPLVPSPKTGDGSSDKKNIDRTWNLGDENNKVSHYSKKSMSSHKRGLSGTAIFGFLGHNKTLSISSLQQSILNMSKDPQPMELINELGNHNTVKNNNDDFDHIRENDGENSYLSQVLLKQQEELRIALEKQKEVNEKLEKQLRDNQIQQEKLRKVLEEQEEVAQKLVSGATNSNSKPGSPVILKTPAMQNGRMKDNAIIVTTNSANGGYQFPPPTLISPRMSNTSINGSPSRKYHRQRYPNKSPESNGLNLFSSNSGYLRDSELLSFSPQNYNLNLDGLTYNDHNNTSDKNNNDKKNSTGDNIFRLFEKTSPGGLSISPRINGNSLRSPFLVGTDKSRDDRYAAGTFTPRTQLSPIHKKRESVVSTVSTISQLQDDTEPIHMRNTQNPTLRNANALASSSVLPPIPGSSNNTPIKNSLPQKHVFQHTPVKAPPKNGSNLAPLLNAPDLTDHQLEIKTPIRNNSHCEVESYPQVPPVTHDIHKSPTLHSTSPLPDEIIPRTTPMKITKKPTTLPPGTIDQYVKELPDKLFECLYPNCNKVFKRRYNIRSHIQTHLQDRPYSCDFPGCTKAFVRNHDLIRHKISHNAKKYICPCGKRFNREDALMVHRSRMICTGGKKLEHSINKKLTSPKKSLLDSPHDTSPVKETIARDKDGSVLMKMEEQLRDDMRKHGLLDPPPSTAAHEQNSNRTLSNETDAL A7FCA8C506A2FD75 cbk1-mob2-1 3278920 N/A 3278924 unspecified role unspecified role unspecified role unspecified role Region45-287 Region45-287 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278925 unspecified role unspecified role unspecified role unspecified role Region 251-756 Region 251-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ssd1-cbk1-1 3278921 N/A 3278925 unspecified role unspecified role prey prey Region 352-756 Region 352-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278926 unspecified role unspecified role bait bait Region 211-214 Region 211-214 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 234-238 Region 234-238 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P24276:p.Phe211Ala F211A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P24276:p.Phe211Trp F211W mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P24276:p.Lys212Ala K212A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P24276:p.Phe213Ala F213A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P24276:p.Phe213Trp F213W/Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P24276:p.Pro214Ala P214A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P24276:p.Lys212Gln K212Q/S/T/D/M/F mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P24276:p.Lys212Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P24276:p.Lys212Thr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P24276:p.Lys212Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P24276:p.Lys212Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P24276:p.Lys212Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P24276:p.Phe213Tyr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ssd1-cbk1-2 3278922 N/A 3278925 unspecified role unspecified role prey prey Region 352-756 Region 352-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P53894:p.Tyr687Ala Y687A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278926 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 208-214 Region 208-214 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ace2-cbk1-2 3278923 N/A 3278927 unspecified role unspecified role bait bait Region 272-286 Region 272-286 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P21192:p.Ile272Ala I272A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21192:p.Asn277Ala N277A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21192:p.Tyr283Ala Y283A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21192:p.Phe285Ala F285A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21192:p.Pro286Ala P286A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278925 unspecified role unspecified role prey prey Region 251-756 Region 251-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false