DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ davies-2015-6 ecolx Escherichia coli atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. DIP original experiment: DIP-264942X PLoS biology 2015 IMEx CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation. Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. Only protein-protein interactions imex curation davies-2015-7 ecolx Escherichia coli atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. DIP original experiment: DIP-264940X PLoS biology 2015 IMEx CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation. Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. Only protein-protein interactions imex curation davies-2015-8 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. DIP original experiment: DIP-264944X PLoS biology 2015 IMEx CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation. Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. Only protein-protein interactions imex curation davies-2015-9 ecolx Escherichia coli fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. DIP original experiment: DIP-264946X PLoS biology 2015 IMEx CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation. Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. Only protein-protein interactions imex curation davies-2015-10 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. DIP original experiment: DIP-264943X PLoS biology 2015 IMEx CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation. Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. Only protein-protein interactions imex curation davies-2015-11 ecolx Escherichia coli fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. DIP original experiment: DIP-264945X PLoS biology 2015 IMEx CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation. Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. Only protein-protein interactions imex curation davies-2015-12 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. DIP original experiment: DIP-264941X PLoS biology 2015 IMEx CYK4 promotes antiparallel microtubule bundling by optimizing MKLP1 neck conformation. Davies T., Kodera N., Kaminski Schierle GS., Rees E., Erdelyi M., Kaminski CF., Ando T., Mishima M. Only protein-protein interactions imex curation g5eg83_caeel Kinesin-like protein zen-4 CELE_M03D4.1 M03D4.1 protein protein caeel Caenorhabditis elegans MSSRKRGITPSRDQVRRKKLSIEETDSIEVVCRLCPYTGSTPSLIAIDEGSIQTVLPPAQFRRENAPQVEKVFRFGRVFSENDGQATVFERTSVDLILNLLKGQNSLLFTYGVTGSGKTYTMTGKPTETGTGLLPRTLDVIFNSINNRVEKCIFYPSALNTFEIRATLDAHLKRHQMAADRLSTSREITDRYCEAIKLSGYNDDMVCSVFVTYVEIYNNYCYDLLEDARNGVLTKREIRHDRQQQMYVDGAKDVEVSSSEEALEVFCLGEERRRVSSTLLNKDSSRSHSVFTIKLVMAPRAYETKSVYPTMDSSQIIVSQLCLVDLAGSERAKRTQNVGERLAEANSINQSLMTLRQCIEVLRRNQKSSSQNLEQVPYRQSKLTHLFKNYLEGNGKIRMVICVNPKPDDYDENMSALAFAEESQTIEVKKQVERMPSERIPHSFFTQWNSELDGSVRMEDDGSREIPCPPTFCLTDCNDKDTVDSMYKYARKLSSLQNSSEEGPSSTLLTMIRQYMMEADYQRVEIARLKDSLNDKDEEIKKLRGFCSRYKRENASMKERIASCEQGEQENALVMEKLMEQKMEDRKIIQSQKKAMRNVRGIIDNPSPSVASLRSRFDQENVAHPTAPIQTPPPPYQTPGRAPVFKKRLEATTSTTVMSGSSSGGSGQQGYVNPKYQRRSKSASRLLDHQPLHRVPTGTVLQSRTPANAIRTTKPEMHQLNKSGEYRLTHQEVDDEGNISTNIVKGNVIPTVSGGTAVFFNDIERLTHESPSTRK q9xus9_caeel cyk-4 CELE_K08E3.6 K08E3.6 protein protein caeel Caenorhabditis elegans MKSSTSKEKVCGENSRHIFNMILNSQRPQFDIKDIGMFHLIDEIERLRKLWKDSEESKKRLNADMREAEEALAKARKKLAMFDIDVKDTQKHLRALMEENKALKLDLNVYETREKQLKDAMKNGIFNSLTKEDRDQFKFLHEPLVRTYSKRVQQRHPHLMEDTQDDEDDSEVDYDETGDSFEEVIHLRNGREVRRSSAAGNAVGGKRRSASAHAITAAANSKRSRSRVMTATIDEEPNEGGTPPKRCRDDGSTPHQEMTTTTTTTTTTTIHNSRAQNQDPPRVSLHRQLTRRSLSCGSIPSCDQTPGQTTNNIGLGMSSAILTKSTLDIRTLKRGTPAWTNGTTRDIAMRPHTFIEAGIKAMRKCDKCATALKLATSMKCRDCHQVVHRSCCNKLHLPCIPRPKTMMTPKSALRGAKPGAGEFRLQDFCTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQELIVAANLYSTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHPVKQSQSQAIAGRDATDCHRAMTALFEFDDVYWQRFLGTSAVSMASNQIETARHQDNFALCDRSILGPVTTSPATPLLARSANATRARGAHLLGSMFHD zen_4-1 3278209 N/A 3278216 unspecified role unspecified role unspecified role unspecified role Region 1-601 Region 1-601 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cyk_4-zen_4-1 3278210 N/A 3278217 unspecified role unspecified role unspecified role unspecified role Region 1-120 Region 1-120 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278216 unspecified role unspecified role unspecified role unspecified role Region 1-601 Region 1-601 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cyk_4-zen_4-3 3278211 N/A 3278217 unspecified role unspecified role bait bait Region 1-120 Region 1-120 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3278216 unspecified role unspecified role prey prey Region 435-555 Region 435-555 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false CBD-tag CBD-tag fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false zen_4-cyk_4-2 3278212 N/A 3278216 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 435-555 Region 435-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Venus-tag Venus-tag venus venus fluorescent protein tag venus n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3278217 unspecified role unspecified role unspecified role unspecified role Region 1-120 Region 1-120 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278216 unspecified role unspecified role fluorescence donor fluorescence donor Region 435-555 Region 435-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Venus-tag Venus-tag venus venus fluorescent protein tag venus n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli proximity proximity close-contact colocalization neighbourhood interaction false false false zen_4-cyk_4-4 3278213 N/A 3278217 unspecified role unspecified role prey prey Region 1-120 Region 1-120 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3278216 unspecified role unspecified role bait bait Region 435-555 Region 435-555 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false CBD-tag CBD-tag fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false zen_4-2 3278214 N/A 3278216 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 435-555 Region 435-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Venus-tag Venus-tag venus venus fluorescent protein tag venus n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3278216 unspecified role unspecified role fluorescence donor fluorescence donor Region 435-555 Region 435-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Venus-tag Venus-tag venus venus fluorescent protein tag venus n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false cyk_4-zen_4-5 3278215 N/A 3278217 unspecified role unspecified role unspecified role unspecified role Region 1-120 Region 1-120 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278216 unspecified role unspecified role unspecified role unspecified role Region 435-555 Region 435-555 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false