DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ park-2015-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265387X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions imex curation park-2015-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265389X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions imex curation park-2015-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265388X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions imex curation park-2015-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265390X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions imex curation marh6_human E3 ubiquitin-protein ligase MARCHF6 MARCHF6 Doa10 homolog KIAA0597 MARCH6 Membrane-associated RING finger protein 6 Membrane-associated RING-CH protein VI Protein TEB-4 RING finger protein 176 RING-type E3 ubiquitin transferase MARCHF6 RNF176 TEB4 protein protein human Homo sapiens Human MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAADQPANPPAENAVVGENPDAQDDQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSVLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVMVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIVRKLAAPVISVLLLSLCVPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVVVLMAILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGSSPPPPQSSQE p41220-1 Regulator of G-protein signaling 2 RGS2 G0S8 GIG31 Cell growth-inhibiting gene 31 protein G0/G1 switch regulatory protein 8 protein protein human Homo sapiens Human MQSAMFLAVQHDCRPMDKSAGSGHKSEEKREKMKRTLLKDWKTRLSYFLQNSSTPGKPKTGKKSKQQAFIKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG rgs2-march6-1 3277076 N/A 3277080 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3277081 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false rgs2-march6-2 3277077 N/A 3277080 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3277081 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 1-10 Region 1-10 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false AcM1 AcM1 acmet N-acetyl-L-methionine (2S)-2-acetamido-4-(methylsulfanyl)butanoic acid 2-acetylamino-4-(methylsulfanyl)butanoic acid 2-acetylamino-4-(methylthio)butanoic acid 2-acetylazanyl-4-(methylsulfanyl)butanoic acid MOD_RES N-acetylmethionine N-acetyl-L-methionine N-acetylated L-methionine acetylmethionine methionamine AcMet certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false march6-rgs2-1 3277078 N/A 3277080 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3277081 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P41220-1:p.Met1Pro M1P mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false rgs2-ub-1 3277079 N/A 3277082 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3277081 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false