DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ cunningham-2015-1 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. DIP original experiment: DIP-266088X Nature cell biology 2015 IMEx USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria. Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. Only protein-protein interactions imex curation cunningham-2015-2 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. DIP original experiment: DIP-266089X Nature cell biology 2015 IMEx USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria. Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. Only protein-protein interactions imex curation cunningham-2015-3 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. DIP original experiment: DIP-266091X Nature cell biology 2015 IMEx USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria. Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. Only protein-protein interactions imex curation cunningham-2015-4 in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay predetermined predetermined participant predetermined Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. DIP original experiment: DIP-266090X Nature cell biology 2015 IMEx USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria. Cunningham CN., Baughman JM., Phu L., Tea JS., Yu C., Coons M., Kirkpatrick DS., Bingol B., Corn JE. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ubp7_human Ubiquitin carboxyl-terminal hydrolase 7 USP7 HAUSP Ubiquitin thioesterase 7 Ubiquitin-specific-processing protease 7 Deubiquitinating enzyme 7 Herpesvirus-associated ubiquitin-specific protease protein protein human Homo sapiens Human MNHQQQQQQQKAGEQQLSEPEDMEMEAGDTDDPPRITQNPVINGNVALSDGHNTAEEDMEDDTSWRSEATFQFTVERFSRLSESVLSPPCFVRNLPWKIMVMPRFYPDRPHQKSVGFFLQCNAESDSTSWSCHAQAVLKIINYRDDEKSFSRRISHLFFHKENDWGFSNFMAWSEVTDPEKGFIDDDKVTFEVFVQADAPHGVAWDSKKHTGYVGLKNQGATCYMNSLLQTLFFTNQLRKAVYMMPTEGDDSSKSVPLALQRVFYELQHSDKPVGTKKLTKSFGWETLDSFMQHDVQELCRVLLDNVENKMKGTCVEGTIPKLFRGKMVSYIQCKEVDYRSDRREDYYDIQLSIKGKKNIFESFVDYVAVEQLDGDNKYDAGEHGLQEAEKGVKFLTLPPVLHLQLMRFMYDPQTDQNIKINDRFEFPEQLPLDEFLQKTDPKDPANYILHAVLVHSGDNHGGHYVVYLNPKGDGKWCKFDDDVVSRCTKEEAIEHNYGGHDDDLSVRHCTNAYMLVYIRESKLSEVLQAVTDHDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDFENRRSFKCIWLNSQFREEEITLYPDKHGCVRDLLEECKKAVELGEKASGKLRLLEIVSYKIIGVHQEDELLECLSPATSRTFRIEEIPLDQVDIDKENEMLVTVAHFHKEVFGTFGIPFLLRIHQGEHFREVMKRIQSLLDIQEKEFEKFKFAIVMMGRHQYINEDEYEVNLKDFEPQPGNMSHPRPWLGLDHFNKAPKRSRYTYLEKAIKIHN ubp30_human Ubiquitin carboxyl-terminal hydrolase 30 USP30 Ubiquitin-specific-processing protease 30 Deubiquitinating enzyme 30 Ubiquitin thioesterase 30 protein protein human Homo sapiens Human MLSSRAEAAMTAADRAIQRFLRTGAAVRYKVMKNWGVIGGIAAALAAGIYVIWGPITERKKRRKGLVPGLVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHLLKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHPFHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPGPTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPVVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLSTSNQWLWVSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE ub-usp7-1 3274811 N/A 3274815 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3274816 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage false false false ub-usp30-2 3274812 N/A 3274817 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3274815 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli protein cleavage protein cleavage false false false usp30-ub-1 3274813 N/A 3274817 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3274815 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false ub-usp30-3 3274814 N/A 3274817 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3274815 enzyme target enzyme target substrate substrate unspecified role unspecified role K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false K-Ub K-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli deubiquitination deubiquitination reaction deubiquitination false false false