DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ delto-2015-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. DIP original experiment: DIP-264746X Structure (London, England : 1993) 2015 IMEx The LisH motif of muskelin is crucial for oligomerization and governs intracellular localization. Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. Only protein-protein interactions imex curation delto-2015-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. DIP original experiment: DIP-264742X Structure (London, England : 1993) 2015 IMEx The LisH motif of muskelin is crucial for oligomerization and governs intracellular localization. Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. Only protein-protein interactions imex curation delto-2015-3 ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. DIP original experiment: DIP-264743X Structure (London, England : 1993) 2015 IMEx The LisH motif of muskelin is crucial for oligomerization and governs intracellular localization. Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. Only protein-protein interactions imex curation delto-2015-4 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. DIP original experiment: DIP-264745X Structure (London, England : 1993) 2015 IMEx The LisH motif of muskelin is crucial for oligomerization and governs intracellular localization. Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. Only protein-protein interactions imex curation delto-2015-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. DIP original experiment: DIP-264744X Structure (London, England : 1993) 2015 IMEx The LisH motif of muskelin is crucial for oligomerization and governs intracellular localization. Delto CF., Heisler FF., Kuper J., Sander B., Kneussel M., Schindelin H. Only protein-protein interactions imex curation gbra1_mouse Gamma-aminobutyric acid receptor subunit alpha-1 GABA(A) receptor subunit alpha-1 Gabra1 Gabra-1 protein protein mouse Mus musculus Mouse MKKSRGLSDYLWAWTLILSTLSGRSYGQPSQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ mkln1_mouse Muskelin Mkln1 protein protein mouse Mus musculus Mouse MAAGGAVAVAPECRLLPYALHKWSSFSSTYLPENILVDKPNDQSSRWSSESNYPPQYLILKLERPAIVQNITFGKYEKTHVCNLKKFKVFGGMNEENMTELLSSGLKNDYNKETFTLKHKIDEQMFPCRFIKIVPLLSWGPSFNFSIWYVELSGIDDPDIVQPCLNWYSKYREQEAIRLCLKHFRQHNYTEAFESLQKKTKIALEHPMLTDMHDKLVLKGDFDACEELIEKAVNDGLFNQYISQQEYKPRWSQIIPKSTKGDGEDNRPGMRGGHQMVIDVQTETVYLFGGWDGTQDLADFWAYSVKENQWTCISRDTEKENGPSARSCHKMCIDIQRRQIYTLGRYLDSSVRNSKSLKSDFYRYDIDTNTWMLLSEDTAADGGPKLVFDHQMCMDSEKHMIYTFGGRILTCNGSVDDSRASEPQFSGLFAFNCQCQTWKLLREDSCNAGPEDIQSRIGHCMLFHSKNRCLYVFGGQRSKTYLNDFFSYDVDSDHVDIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKEKREENVRNSFWIYDIVRNSWSCVYKNDQATKDNLSKSLQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSCSPKMRLDDFWSLKLCRPSKDYLLRHCKYLIRKHRFEEKAQMDPLSALKYLQNDLYITVDHSDPEETKEFQLLASALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPDSMTPPKGNLVDLITL gabra1-mkln1-1 3274262 N/A 3274267 unspecified role unspecified role unspecified role unspecified role Region 334-420 Region 334-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3274268 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false mkln1-1 3274263 N/A 3274268 unspecified role unspecified role unspecified role unspecified role Region 12-205 Region 12-205 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mkln1-2 3274264 N/A 3274268 unspecified role unspecified role unspecified role unspecified role Region 1-205 Region 1-205 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 157-735 Region 157-735 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mkln1-3 3274265 N/A 3274268 unspecified role unspecified role unspecified role unspecified role Region 1-205 Region 1-205 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 157-735 Region 157-735 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O89050:p.[Phe184Glu;Leu196Gln] F184E/L196Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O89050:p.[Lys182Ala;His183Ala] K182A/H183A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Region 626-735 Region 626-735 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O89050:p.[Asn144Arg;Phe184Glu;Leu196Gln] F184E/L196Q/N144R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O89050:p.Asn144Arg N144R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mkln1-4 3274266 N/A 3274268 unspecified role unspecified role unspecified role unspecified role Region 1-205 Region 1-205 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 157-735 Region 157-735 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O89050:p.[Phe184Glu;Leu196Gln] F184E/L196Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O89050:p.[Lys182Ala;His183Ala] K182A/H183A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Region 626-735 Region 626-735 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false O89050:p.[Asn144Arg;Phe184Glu;Leu196Gln] F184E/L196Q/N144R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O89050:p.Asn144Arg N144R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false